ASG1_YEAST
ID ASG1_YEAST Reviewed; 964 AA.
AC P40467; D6VVF7; Q45U13;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 171.
DE RecName: Full=Activator of stress genes 1;
GN Name=ASG1; OrderedLocusNames=YIL130W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANTS ASN-77; SER-149; PHE-353;
RP SER-682; PHE-707; THR-770; SER-811; ASN-ASP-ASN-ASN-ASN-852 INS; ASN-887
RP INS AND 926-ASN--GLN-933 DEL.
RC STRAIN=SK1;
RX PubMed=16273108; DOI=10.1038/ng1674;
RA Deutschbauer A.M., Davis R.W.;
RT "Quantitative trait loci mapped to single-nucleotide resolution in yeast.";
RL Nat. Genet. 37:1333-1340(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169870;
RA Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D.,
RA Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E.,
RA Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C.,
RA Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V.,
RA Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IX.";
RL Nature 387:84-87(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP DISRUPTION PHENOTYPE, AND FUNCTION.
RX PubMed=11353088; DOI=10.1093/nar/29.10.2181;
RA Akache B., Wu K., Turcotte B.;
RT "Phenotypic analysis of genes encoding yeast zinc cluster proteins.";
RL Nucleic Acids Res. 29:2181-2190(2001).
RN [5]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-963, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ADR376;
RX PubMed=17330950; DOI=10.1021/pr060559j;
RA Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,
RA Elias J.E., Gygi S.P.;
RT "Large-scale phosphorylation analysis of alpha-factor-arrested
RT Saccharomyces cerevisiae.";
RL J. Proteome Res. 6:1190-1197(2007).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-166 AND SER-963, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [9]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-186 AND SER-963, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
RN [10]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- FUNCTION: Probable transcription factor involved in the stress
CC response. {ECO:0000269|PubMed:11353088}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00227,
CC ECO:0000269|PubMed:14562095}.
CC -!- DISRUPTION PHENOTYPE: No growth on non-fermentable carbon sources like
CC glycerol and lactate and sensitivity to calcofluor white.
CC {ECO:0000269|PubMed:11353088}.
CC -!- MISCELLANEOUS: Present with 396 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the ASG1 family. {ECO:0000305}.
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DR EMBL; DQ115392; AAZ22497.1; -; Genomic_DNA.
DR EMBL; Z38059; CAA86148.1; -; Genomic_DNA.
DR EMBL; BK006942; DAA08423.1; -; Genomic_DNA.
DR PIR; S48404; S48404.
DR RefSeq; NP_012136.1; NM_001179478.1.
DR AlphaFoldDB; P40467; -.
DR SMR; P40467; -.
DR BioGRID; 34861; 66.
DR DIP; DIP-820N; -.
DR IntAct; P40467; 4.
DR MINT; P40467; -.
DR STRING; 4932.YIL130W; -.
DR iPTMnet; P40467; -.
DR MaxQB; P40467; -.
DR PaxDb; P40467; -.
DR PRIDE; P40467; -.
DR TopDownProteomics; P40467; -.
DR EnsemblFungi; YIL130W_mRNA; YIL130W; YIL130W.
DR GeneID; 854676; -.
DR KEGG; sce:YIL130W; -.
DR SGD; S000001392; ASG1.
DR VEuPathDB; FungiDB:YIL130W; -.
DR eggNOG; ENOG502QSY2; Eukaryota.
DR GeneTree; ENSGT00940000176737; -.
DR HOGENOM; CLU_010084_1_0_1; -.
DR InParanoid; P40467; -.
DR OMA; MAADRCA; -.
DR BioCyc; YEAST:G3O-31381-MON; -.
DR PRO; PR:P40467; -.
DR Proteomes; UP000002311; Chromosome IX.
DR RNAct; P40467; protein.
DR GO; GO:0005634; C:nucleus; HDA:SGD.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR GO; GO:0043565; F:sequence-specific DNA binding; HDA:SGD.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR CDD; cd00067; GAL4; 1.
DR Gene3D; 4.10.240.10; -; 1.
DR InterPro; IPR007219; Transcription_factor_dom_fun.
DR InterPro; IPR001138; Zn2-C6_fun-type_DNA-bd.
DR InterPro; IPR036864; Zn2-C6_fun-type_DNA-bd_sf.
DR Pfam; PF04082; Fungal_trans; 1.
DR Pfam; PF00172; Zn_clus; 1.
DR SMART; SM00906; Fungal_trans; 1.
DR SMART; SM00066; GAL4; 1.
DR SUPFAM; SSF57701; SSF57701; 1.
DR PROSITE; PS00463; ZN2_CY6_FUNGAL_1; 1.
DR PROSITE; PS50048; ZN2_CY6_FUNGAL_2; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Metal-binding; Nucleus; Phosphoprotein; Reference proteome;
KW Stress response; Transcription; Transcription regulation; Zinc.
FT CHAIN 1..964
FT /note="Activator of stress genes 1"
FT /id="PRO_0000114997"
FT DNA_BIND 21..47
FT /note="Zn(2)-C6 fungal-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00227"
FT REGION 104..125
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 190..225
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 733..764
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 800..900
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 166
FT /note="Phosphoserine; by ATM or ATR"
FT /evidence="ECO:0007744|PubMed:18407956"
FT MOD_RES 186
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT MOD_RES 963
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17330950,
FT ECO:0007744|PubMed:18407956, ECO:0007744|PubMed:19779198"
FT VARIANT 77
FT /note="S -> N (in strain: SK1)"
FT /evidence="ECO:0000269|PubMed:16273108"
FT VARIANT 149
FT /note="N -> S (in strain: SK1)"
FT /evidence="ECO:0000269|PubMed:16273108"
FT VARIANT 353
FT /note="I -> F (in strain: SK1)"
FT /evidence="ECO:0000269|PubMed:16273108"
FT VARIANT 682
FT /note="F -> S (in strain: SK1)"
FT /evidence="ECO:0000269|PubMed:16273108"
FT VARIANT 707
FT /note="S -> F (in strain: SK1)"
FT /evidence="ECO:0000269|PubMed:16273108"
FT VARIANT 770
FT /note="I -> T (in strain: SK1)"
FT /evidence="ECO:0000269|PubMed:16273108"
FT VARIANT 811
FT /note="N -> S (in strain: SK1)"
FT /evidence="ECO:0000269|PubMed:16273108"
FT VARIANT 852
FT /note="D -> NDNNN (in strain: SK1)"
FT VARIANT 887
FT /note="N -> NN (in strain: SK1)"
FT /evidence="ECO:0000269|PubMed:16273108"
FT VARIANT 926..933
FT /note="Missing (in strain: SK1)"
FT /evidence="ECO:0000269|PubMed:16273108"
SQ SEQUENCE 964 AA; 108781 MW; AD5ABE59E4B022CC CRC64;
MPEQAQQGEQ SVKRRRVTRA CDECRKKKVK CDGQQPCIHC TVYSYECTYK KPTKRTQNSG
NSGVLTLGNV TTGPSSSTVV AAAASNPNKL LSNIKTERAI LPGASTIPAS NNPSKPRKYK
TKSTRLQSKI DRYKQIFDEV FPQLPDIDNL DIPVFLQIFH NFKRDSQSFL DDTVKEYTLI
VNDSSSPIQP VLSSNSKNST PDEFLPNMKS DSNSASSNRE QDSVDTYSNI PVGREIKIIL
PPKAIALQFV KSTWEHCCVL LRFYHRPSFI RQLDELYETD PNNYTSKQMQ FLPLCYAAIA
VGALFSKSIV SNDSSREKFL QDEGYKYFIA ARKLIDITNA RDLNSIQAIL MLIIFLQCSA
RLSTCYTYIG VAMRSALRAG FHRKLSPNSG FSPIEIEMRK RLFYTIYKLD VYINAMLGLP
RSISPDDFDQ TLPLDLSDEN ITEVAYLPEN QHSVLSSTGI SNEHTKLFLI LNEIISELYP
IKKTSNIISH ETVTSLELKL RNWLDSLPKE LIPNAENIDP EYERANRLLH LSFLHVQIIL
YRPFIHYLSR NMNAENVDPL CYRRARNSIA VARTVIKLAK EMVSNNLLTG SYWYACYTIF
YSVAGLLFYI HEAQLPDKDS AREYYDILKD AETGRSVLIQ LKDSSMAASR TYNLLNQIFE
KLNSKTIQLT ALHSSPSNES AFLVTNNSSA LKPHLGDSLQ PPVFFSSQDT KNSFSLAKSE
ESTNDYAMAN YLNNTPISEN PLNEAQQQDQ VSQGTTNMSN ERDPNNFLSI DIRLDNNGQS
NILDATDDVF IRNDGDIPTN SAFDFSSSKS NASNNSNPDT INNNYNNVSG KNNNNNNITN
NSNNNHNNNN NDNNNNNNNN NNNNNNNNNS GNSSNNNNNN NNNKNNNDFG IKIDNNSPSY
EGFPQLQIPL SQDNLNIEDK EEMSPNIEIK NEQNMTDSND ILGVFDQLDA QLFGKYLPLN
YPSE
