P2RY4_RAT
ID P2RY4_RAT Reviewed; 361 AA.
AC O35811;
DT 27-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=P2Y purinoceptor 4;
DE Short=P2Y4;
GN Name=P2ry4; Synonyms=P2y4;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Sprague-Dawley; TISSUE=Liver;
RA Bogdanov Y.D., Wildman S., King B.F., Burntock G.;
RL Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Sprague-Dawley; TISSUE=Brain;
RX PubMed=9751165; DOI=10.1046/j.1471-4159.1998.71041348.x;
RA Webb T.E., Henderson D., Roberts J.A., Barnard E.A.;
RT "Molecular cloning and characterization of the rat P2Y4 receptor.";
RL J. Neurochem. 71:1348-1357(1998).
CC -!- FUNCTION: Receptor for ATP and UTP coupled to G-proteins that activate
CC a phosphatidylinositol-calcium second messenger system. Not activated
CC by ADP or UDP.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC -!- TISSUE SPECIFICITY: Widely expressed at low levels. In brain, higher
CC expression in the pineal gland and ventricular system.
CC -!- PTM: Phosphorylation of Ser-329 and Ser-330 is a key step in agonist-
CC dependent desensitization and loss of surface P2RY4. This
CC phosphorylation does not involve PKC, nor other calcium-activated
CC kinases (By similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC {ECO:0000255|PROSITE-ProRule:PRU00521}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; Y14705; CAA75007.1; -; Genomic_DNA.
DR EMBL; Y11433; CAA72241.1; -; mRNA.
DR RefSeq; NP_113868.1; NM_031680.1.
DR RefSeq; XP_006257155.1; XM_006257093.3.
DR RefSeq; XP_006257156.1; XM_006257094.3.
DR RefSeq; XP_006257157.1; XM_006257095.3.
DR RefSeq; XP_017457635.1; XM_017602146.1.
DR RefSeq; XP_017457636.1; XM_017602147.1.
DR AlphaFoldDB; O35811; -.
DR SMR; O35811; -.
DR STRING; 10116.ENSRNOP00000039027; -.
DR BindingDB; O35811; -.
DR ChEMBL; CHEMBL2124; -.
DR DrugCentral; O35811; -.
DR GuidetoPHARMACOLOGY; 325; -.
DR GlyGen; O35811; 1 site.
DR PhosphoSitePlus; O35811; -.
DR PaxDb; O35811; -.
DR PRIDE; O35811; -.
DR Ensembl; ENSRNOT00000045907; ENSRNOP00000039027; ENSRNOG00000070777.
DR Ensembl; ENSRNOT00000096316; ENSRNOP00000094459; ENSRNOG00000070777.
DR Ensembl; ENSRNOT00000099755; ENSRNOP00000085052; ENSRNOG00000070777.
DR Ensembl; ENSRNOT00000112073; ENSRNOP00000082634; ENSRNOG00000070777.
DR GeneID; 63843; -.
DR KEGG; rno:63843; -.
DR UCSC; RGD:61798; rat.
DR CTD; 5030; -.
DR RGD; 61798; P2ry4.
DR eggNOG; ENOG502QSTF; Eukaryota.
DR GeneTree; ENSGT01030000234621; -.
DR HOGENOM; CLU_009579_8_2_1; -.
DR InParanoid; O35811; -.
DR OMA; HWPFGTG; -.
DR OrthoDB; 1077455at2759; -.
DR PhylomeDB; O35811; -.
DR TreeFam; TF350009; -.
DR Reactome; R-RNO-417957; P2Y receptors.
DR Reactome; R-RNO-418594; G alpha (i) signalling events.
DR PRO; PR:O35811; -.
DR Proteomes; UP000002494; Chromosome X.
DR Bgee; ENSRNOG00000002953; Expressed in esophagus and 17 other tissues.
DR Genevisible; O35811; RN.
DR GO; GO:0016324; C:apical plasma membrane; IDA:RGD.
DR GO; GO:0016323; C:basolateral plasma membrane; IDA:RGD.
DR GO; GO:0098978; C:glutamatergic synapse; IDA:SynGO.
DR GO; GO:0099059; C:integral component of presynaptic active zone membrane; IDA:SynGO.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IDA:RGD.
DR GO; GO:0045028; F:G protein-coupled purinergic nucleotide receptor activity; IEA:InterPro.
DR GO; GO:0045030; F:G protein-coupled UTP receptor activity; ISO:RGD.
DR GO; GO:0038023; F:signaling receptor activity; IBA:GO_Central.
DR GO; GO:0071380; P:cellular response to prostaglandin E stimulus; ISO:RGD.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0099509; P:regulation of presynaptic cytosolic calcium ion concentration; IDA:SynGO.
DR GO; GO:2000300; P:regulation of synaptic vesicle exocytosis; IDA:SynGO.
DR GO; GO:0030321; P:transepithelial chloride transport; ISO:RGD.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR000018; P2Y4.
DR PANTHER; PTHR24231:SF21; PTHR24231:SF21; 1.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PRINTS; PR01066; P2Y4PRNOCPTR.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Disulfide bond; G-protein coupled receptor; Glycoprotein;
KW Membrane; Phosphoprotein; Receptor; Reference proteome; Transducer;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..361
FT /note="P2Y purinoceptor 4"
FT /id="PRO_0000070023"
FT TOPO_DOM 1..30
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 31..58
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 59..68
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 69..91
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 92..108
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 109..127
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 128..149
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 150..170
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 171..192
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 193..218
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 219..242
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 243..265
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 266..283
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 284..305
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 306..361
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..17
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 175
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 104..181
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
SQ SEQUENCE 361 AA; 40894 MW; 0377F96E54B449A3 CRC64;
MTSAESLLFT SLGPSPSSGD GDCRFNEEFK FILLPMSYAV VFVLGLALNA PTLWLFLFRL
RPWDATATYM FHLALSDTLY VLSLPTLVYY YAARNHWPFG TGLCKFVRFL FYWNLYCSVL
FLTCISVHRY LGICHPLRAI RWGRPRFASL LCLGVWLVVA GCLVPNLFFV TTNANGTTIL
CHDTTLPEEF DHYVYFSSAV MVLLFGLPFL ITLVCYGLMA RRLYRPLPGA GQSSSRLRSL
RTIAVVLTVF AVCFVPFHIT RTIYYQARLL QADCHVLNIV NVVYKVTRPL ASANSCLDPV
LYLFTGDKYR NQLQQLCRGS KPKPRTAASS LALVTLHEES ISRWADTHQD STFSAYEGDR
L
