P2SAF_ARATH
ID P2SAF_ARATH Reviewed; 403 AA.
AC O82660; Q56Z72;
DT 15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 149.
DE RecName: Full=Photosystem II stability/assembly factor HCF136, chloroplastic;
DE Flags: Precursor;
GN Name=HCF136; OrderedLocusNames=At5g23120; ORFNames=MYJ24.11;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION
RP PHENOTYPE.
RC STRAIN=cv. Wassilewskija; TISSUE=Leaf;
RX PubMed=9736608; DOI=10.1093/emboj/17.18.5286;
RA Meurer J., Pluecken H., Kowallik K.V., Westhoff P.;
RT "A nuclear-encoded protein of prokaryotic origin is essential for the
RT stability of photosystem II in Arabidopsis thaliana.";
RL EMBO J. 17:5286-5297(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9405937; DOI=10.1093/dnares/4.4.291;
RA Kotani H., Nakamura Y., Sato S., Kaneko T., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. II. Sequence
RT features of the regions of 1,044,062 bp covered by thirteen physically
RT assigned P1 clones.";
RL DNA Res. 4:291-300(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 333-403.
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP PROTEIN SEQUENCE OF 79-93, AND SUBCELLULAR LOCATION.
RX PubMed=11719511; DOI=10.1074/jbc.m108575200;
RA Schubert M., Petersson U.A., Haas B.J., Funk C., Schroeder W.P.,
RA Kieselbach T.;
RT "Proteome map of the chloroplast lumen of Arabidopsis thaliana.";
RL J. Biol. Chem. 277:8354-8365(2002).
RN [7]
RP PROTEIN SEQUENCE OF N-TERMINUS, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=11826309; DOI=10.1105/tpc.010304;
RA Peltier J.-B., Emanuelsson O., Kalume D.E., Ytterberg J., Friso G.,
RA Rudella A., Liberles D.A., Soederberg L., Roepstorff P., von Heijne G.,
RA van Wijk K.J.;
RT "Central functions of the lumenal and peripheral thylakoid proteome of
RT Arabidopsis determined by experimentation and genome-wide prediction.";
RL Plant Cell 14:211-236(2002).
RN [8]
RP IN VITRO IMPORT INTO PEA CHLOROPLASTS, AND MUTAGENESIS OF 55-ARG-ARG-56.
RX PubMed=10664464; DOI=10.1016/s0014-5793(00)01129-7;
RA Hynds P.J., Pluecken H., Westhoff P., Robinson C.;
RT "Different lumen-targeting pathways for nuclear-encoded versus
RT cyanobacterial/plastid-encoded Hcf136 proteins.";
RL FEBS Lett. 467:97-100(2000).
RN [9]
RP FUNCTION IN EARLY ASSEMBLY OF PHOTOSYSTEM II.
RX PubMed=12459468; DOI=10.1016/s0014-5793(02)03634-7;
RA Pluecken H., Mueller B., Grohmann D., Westhoff P., Eichacker L.A.;
RT "The HCF136 protein is essential for assembly of the photosystem II
RT reaction center in Arabidopsis thaliana.";
RL FEBS Lett. 532:85-90(2002).
RN [10]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION [LARGE SCALE
RP ANALYSIS].
RX PubMed=18431481; DOI=10.1371/journal.pone.0001994;
RA Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q.,
RA van Wijk K.J.;
RT "Sorting signals, N-terminal modifications and abundance of the chloroplast
RT proteome.";
RL PLoS ONE 3:E1994-E1994(2008).
RN [11]
RP INTERACTION WITH PAM68.
RX PubMed=20923938; DOI=10.1105/tpc.110.077453;
RA Armbruster U., Zuhlke J., Rengstl B., Kreller R., Makarenko E., Ruhle T.,
RA Schunemann D., Jahns P., Weisshaar B., Nickelsen J., Leister D.;
RT "The Arabidopsis thylakoid protein PAM68 is required for efficient D1
RT biogenesis and photosystem II assembly.";
RL Plant Cell 22:3439-3460(2010).
CC -!- FUNCTION: Essential for photosystem II (PSII) biogenesis; required for
CC assembly of an early intermediate in PSII assembly that includes D2
CC (psbD) and cytochrome b559. Has been suggested (PubMed:11826309) to be
CC required for chlorophyll a binding. {ECO:0000269|PubMed:11826309,
CC ECO:0000269|PubMed:12459468, ECO:0000269|PubMed:9736608}.
CC -!- SUBUNIT: Interacts with PAM68. {ECO:0000269|PubMed:20923938}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid lumen
CC {ECO:0000269|PubMed:11719511, ECO:0000269|PubMed:18431481,
CC ECO:0000269|PubMed:9736608}. Note=Restricted to the stromal lamellae
CC (PubMed:9736608). Translocation into the thylakoid lumen occurs via the
CC Tat pathway (Probable) (PubMed:10664464). {ECO:0000269|PubMed:10664464,
CC ECO:0000269|PubMed:9736608, ECO:0000305|PubMed:11719511,
CC ECO:0000305|PubMed:11826309}.
CC -!- TISSUE SPECIFICITY: Expression in green tissue, not roots.
CC -!- DEVELOPMENTAL STAGE: Accumulates also in dark-grown seedlings.
CC {ECO:0000269|PubMed:9736608}.
CC -!- DOMAIN: A 7-bladed beta-propeller torus, about 54 by 55 Angstroms, with
CC a depth of about 25 Angstroms and a central pore.
CC {ECO:0000250|UniProtKB:M1VJU3}.
CC -!- DISRUPTION PHENOTYPE: Seedling lethal when homozygous, has pale
CC cotyledons but never develops true leaves. On sucrose-supplemented
CC medium PSII is completely inactive; D1, D2 CP43 and CP47 are present in
CC very low amounts. {ECO:0000269|PubMed:9736608}.
CC -!- SIMILARITY: Belongs to the Ycf48 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; Y15628; CAA75723.1; -; mRNA.
DR EMBL; AB006708; BAB09829.1; -; Genomic_DNA.
DR EMBL; CP002688; AED93124.1; -; Genomic_DNA.
DR EMBL; AY045691; AAK74049.1; -; mRNA.
DR EMBL; BT004549; AAO42795.1; -; mRNA.
DR EMBL; AK221097; BAD94976.1; -; mRNA.
DR PIR; T51828; T51828.
DR RefSeq; NP_197703.1; NM_122218.3.
DR AlphaFoldDB; O82660; -.
DR SMR; O82660; -.
DR BioGRID; 17651; 4.
DR IntAct; O82660; 4.
DR STRING; 3702.AT5G23120.1; -.
DR World-2DPAGE; 0003:O82660; -.
DR PaxDb; O82660; -.
DR PRIDE; O82660; -.
DR ProteomicsDB; 248814; -.
DR EnsemblPlants; AT5G23120.1; AT5G23120.1; AT5G23120.
DR GeneID; 832376; -.
DR Gramene; AT5G23120.1; AT5G23120.1; AT5G23120.
DR KEGG; ath:AT5G23120; -.
DR Araport; AT5G23120; -.
DR TAIR; locus:2178262; AT5G23120.
DR eggNOG; KOG3511; Eukaryota.
DR HOGENOM; CLU_057027_0_0_1; -.
DR InParanoid; O82660; -.
DR PhylomeDB; O82660; -.
DR PRO; PR:O82660; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; O82660; baseline and differential.
DR Genevisible; O82660; AT.
DR GO; GO:0009507; C:chloroplast; HDA:TAIR.
DR GO; GO:0009941; C:chloroplast envelope; HDA:TAIR.
DR GO; GO:0009570; C:chloroplast stroma; HDA:TAIR.
DR GO; GO:0009533; C:chloroplast stromal thylakoid; IDA:TAIR.
DR GO; GO:0009534; C:chloroplast thylakoid; HDA:TAIR.
DR GO; GO:0009543; C:chloroplast thylakoid lumen; IDA:TAIR.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; HDA:TAIR.
DR GO; GO:0009523; C:photosystem II; IEA:UniProtKB-KW.
DR GO; GO:0009536; C:plastid; HDA:TAIR.
DR GO; GO:0009579; C:thylakoid; HDA:TAIR.
DR GO; GO:0031977; C:thylakoid lumen; HDA:TAIR.
DR GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-KW.
DR GO; GO:0009657; P:plastid organization; IMP:TAIR.
DR GO; GO:0065003; P:protein-containing complex assembly; IMP:TAIR.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR028203; PSII_CF48-like_dom.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR016705; Ycf48/Hcf136.
DR Pfam; PF14870; PSII_BNR; 1.
DR PIRSF; PIRSF017875; PSII_HCF136; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Direct protein sequencing; Photosynthesis; Photosystem II;
KW Plastid; Reference proteome; Thylakoid; Transit peptide.
FT TRANSIT 1..53
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT TRANSIT 54..78
FT /note="Thylakoid"
FT /evidence="ECO:0000269|PubMed:11719511,
FT ECO:0000269|PubMed:11826309"
FT CHAIN 79..403
FT /note="Photosystem II stability/assembly factor HCF136,
FT chloroplastic"
FT /id="PRO_0000005341"
FT REGION 31..50
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 55..56
FT /note="RR->KK: Protein is imported into chloroplasts but is
FT unable to translocate into the thylakoid lumen."
FT /evidence="ECO:0000269|PubMed:10664464"
SQ SEQUENCE 403 AA; 44104 MW; 11079552F817FF9D CRC64;
MASLQLCDGY LLFKPSVSPR FLSQRISHRL IPKASSSPPP SPSPSSSSSS LSFSRRELLY
QSAAVSLSLS SIVGPARADE QLSEWERVFL PIDPGVVLLD IAFVPDEPSR GFLLGTRQTL
LETKDGGSTW NPRSIPSAEE EDFNYRFNSI SFKGKEGWII GKPAILLYTA DAGENWDRIP
LSSQLPGDMV FIKATEDKSA EMVTDEGAIY VTSNRGYNWK AAIQETVSAT LNRTVSSGIS
GASYYTGTFS AVNRSPDGRY VAVSSRGNFF LTWEPGQPYW QPHNRAVARR IQNMGWRADG
GLWLLVRGGG LYLSKGTGIT EEFEEVPVQS RGFGILDVGY RSEEEAWAAG GSGILLRTRN
GGKSWNRDKA ADNIAANLYA VKFVDDKKGF VLGNDGVLLR YVG
