P2XC_DICDI
ID P2XC_DICDI Reviewed; 368 AA.
AC Q553Y0;
DT 15-DEC-2009, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 25-MAY-2022, entry version 91.
DE RecName: Full=P2X receptor C;
DE Short=P2XC;
GN Name=p2xC; ORFNames=DDB_G0275191;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=12097910; DOI=10.1038/nature00847;
RA Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA Noegel A.A.;
RT "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL Nature 418:79-85(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3]
RP FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=19833731; DOI=10.1074/jbc.m109.045674;
RA Ludlow M.J., Durai L., Ennion S.J.;
RT "Functional characterization of intracellular Dictyostelium discoideum P2X
RT receptors.";
RL J. Biol. Chem. 284:35227-35239(2009).
CC -!- FUNCTION: P2X receptors are ligand-gated ion channels that play a role
CC in intracellular calcium signaling. ATP does not evoke inward currents
CC in p2xC. Not essential for osmoregulation.
CC {ECO:0000269|PubMed:19833731}.
CC -!- SUBCELLULAR LOCATION: Contractile vacuole membrane
CC {ECO:0000269|PubMed:19833731}. Note=Ligand binding domain within the
CC lumen of the vacuole.
CC -!- DISRUPTION PHENOTYPE: Null cells are still capable of osmoregulation
CC and do not show any noticeable differences in their sensitivity to
CC hypotonic conditions. Quintuple p2xA/p2xB/p2xC/p2xD/p2xE null cells
CC displayed slight delay in their osmoregulatory response, but are still
CC capable of regulating their cell volume in water. Extracellular
CC purinergic response to ATP persists in the quintuple null cells and
CC p2xC single null strains with no alteration in the kinetics of the
CC response, but the magnitude of the response is lower. Responses to the
CC calmodulin antagonist calmidazolium are reduced and intracellular
CC calcium signaling is disrupted in quintuple null cells. The presence of
CC copper prevented both wild type and quintuple null cells from
CC undergoing an osmoregulatory decrease in cell volume. No obvious
CC morphological phenotype was apparent in the p2xC or quintuple p2x null
CC strains. The quintuple null strain however did grow slightly slower
CC than wild type in shaking axenic cultures.
CC {ECO:0000269|PubMed:19833731}.
CC -!- SIMILARITY: Belongs to the P2X receptor family. {ECO:0000305}.
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DR EMBL; AAFI02000013; EAL69876.1; -; Genomic_DNA.
DR RefSeq; XP_643831.1; XM_638739.1.
DR AlphaFoldDB; Q553Y0; -.
DR PaxDb; Q553Y0; -.
DR EnsemblProtists; EAL69876; EAL69876; DDB_G0275191.
DR GeneID; 8619878; -.
DR KEGG; ddi:DDB_G0275191; -.
DR dictyBase; DDB_G0275191; p2xC.
DR eggNOG; ENOG502RE1D; Eukaryota.
DR HOGENOM; CLU_060033_0_0_1; -.
DR InParanoid; Q553Y0; -.
DR OMA; HASICAQ; -.
DR PhylomeDB; Q553Y0; -.
DR Reactome; R-DDI-139853; Elevation of cytosolic Ca2+ levels.
DR Reactome; R-DDI-418346; Platelet homeostasis.
DR Reactome; R-DDI-6798695; Neutrophil degranulation.
DR Reactome; R-DDI-844456; The NLRP3 inflammasome.
DR PRO; PR:Q553Y0; -.
DR Proteomes; UP000002195; Chromosome 2.
DR GO; GO:0031164; C:contractile vacuolar membrane; IDA:dictyBase.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0035381; F:ATP-gated ion channel activity; IBA:GO_Central.
DR GO; GO:0140417; F:ATP-sensitive calcium-release channel activity; IGI:dictyBase.
DR GO; GO:0070588; P:calcium ion transmembrane transport; IGI:dictyBase.
DR GO; GO:0071476; P:cellular hypotonic response; IGI:dictyBase.
DR GO; GO:0050848; P:regulation of calcium-mediated signaling; IGI:dictyBase.
PE 3: Inferred from homology;
KW Ion channel; Ion transport; Ligand-gated ion channel; Membrane; Receptor;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport; Vacuole.
FT CHAIN 1..368
FT /note="P2X receptor C"
FT /id="PRO_0000390413"
FT TOPO_DOM 1..24
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 25..45
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 46..368
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT REGION 282..295
FT /note="Pore-forming motif"
FT /evidence="ECO:0000255"
SQ SEQUENCE 368 AA; 41756 MW; 2B3ECD56CB108700 CRC64;
MLDWDSILAY NTIKVVRIRD RRLGILHLIF MIAIISYVVI YSAIIKKGYL SIEEPVGSIR
TSLWSPNQFN GNESYCKNNA KPYPYEKLDC VYYDDALALY PIGDDVGFTA STRIEISDQK
ANCSLMNPSC KFKTFNYSNV YLADIESFTV LIDHTMYAPS SQIQFNGGDL SGYVLDQNGN
EIQLNESVNV IGVEGKPDVL EISKLLEFAG VNLDDPSLTN SSNPIRYDGC VLFVFIEYSN
TFSYDLNKIK YVYSIKLVDD TIYNIPEVVI LDDVNSRLYY KRHAIRLIFI QTGVIGSFNF
QSLLLTLVSG LGLLTVSTLI VDQLAIRFLP QRKSYSSLKF QTTESFKMKK KIVNDDGEDK
LYHNIEAL
