ID   P2XD_DICDI              Reviewed;         407 AA.
AC   Q54J33;
DT   15-DEC-2009, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 93.
DE   RecName: Full=P2X receptor D;
DE            Short=P2XD;
GN   Name=p2xD; ORFNames=DDB_G0288335;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [2]
RP   FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX   PubMed=19833731; DOI=10.1074/jbc.m109.045674;
RA   Ludlow M.J., Durai L., Ennion S.J.;
RT   "Functional characterization of intracellular Dictyostelium discoideum P2X
RT   receptors.";
RL   J. Biol. Chem. 284:35227-35239(2009).
CC   -!- FUNCTION: P2X receptors are ligand-gated ion channels that play a role
CC       in intracellular calcium signaling. ATP does not evoke inward currents
CC       in p2xD. Not essential for osmoregulation.
CC       {ECO:0000269|PubMed:19833731}.
CC   -!- SUBCELLULAR LOCATION: Contractile vacuole membrane
CC       {ECO:0000269|PubMed:19833731}. Note=Ligand binding domain within the
CC       lumen of the vacuole.
CC   -!- DISRUPTION PHENOTYPE: Null cells are still capable of osmoregulation
CC       and do not show any noticeable differences in their sensitivity to
CC       hypotonic conditions. Quintuple p2xA/p2xB/p2xC/p2xD/p2xE null cells
CC       display slight delay in their osmoregulatory response, but are still
CC       capable of regulating their cell volume in water. Extracellular
CC       purinergic response to ATP persists in the quintuple null cells and
CC       p2xD single null strains with no alteration in the kinetics of the
CC       response, but the magnitude of the response is lower. Responses to the
CC       calmodulin antagonist calmidazolium are reduced and intracellular
CC       calcium signaling is disrupted in quintuple null cells. The presence of
CC       copper prevented both wild type and quintuple null cells from
CC       undergoing an osmoregulatory decrease in cell volume. No obvious
CC       morphological phenotype was apparent in the p2xD or quintuple p2x null
CC       strains. Null cells grow and are also able to develop normally upon
CC       starvation to form fruiting bodies. The quintuple null strain however
CC       did grow slightly slower than wild type in shaking axenic cultures.
CC       {ECO:0000269|PubMed:19833731}.
CC   -!- SIMILARITY: Belongs to the P2X receptor family. {ECO:0000305}.
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DR   EMBL; AAFI02000111; EAL63264.1; -; Genomic_DNA.
DR   RefSeq; XP_636768.1; XM_631676.1.
DR   AlphaFoldDB; Q54J33; -.
DR   PaxDb; Q54J33; -.
DR   EnsemblProtists; EAL63264; EAL63264; DDB_G0288335.
DR   GeneID; 8626571; -.
DR   KEGG; ddi:DDB_G0288335; -.
DR   dictyBase; DDB_G0288335; p2xD.
DR   HOGENOM; CLU_060033_0_0_1; -.
DR   InParanoid; Q54J33; -.
DR   OMA; FTVENTH; -.
DR   PhylomeDB; Q54J33; -.
DR   Reactome; R-DDI-139853; Elevation of cytosolic Ca2+ levels.
DR   Reactome; R-DDI-418346; Platelet homeostasis.
DR   Reactome; R-DDI-6798695; Neutrophil degranulation.
DR   Reactome; R-DDI-844456; The NLRP3 inflammasome.
DR   PRO; PR:Q54J33; -.
DR   Proteomes; UP000002195; Chromosome 5.
DR   GO; GO:0031164; C:contractile vacuolar membrane; IDA:dictyBase.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0035381; F:ATP-gated ion channel activity; IBA:GO_Central.
DR   GO; GO:0140417; F:ATP-sensitive calcium-release channel activity; IGI:dictyBase.
DR   GO; GO:0070588; P:calcium ion transmembrane transport; IGI:dictyBase.
DR   GO; GO:0071476; P:cellular hypotonic response; IGI:dictyBase.
DR   GO; GO:0050848; P:regulation of calcium-mediated signaling; IGI:dictyBase.
PE   3: Inferred from homology;
KW   Ion channel; Ion transport; Ligand-gated ion channel; Membrane; Receptor;
KW   Reference proteome; Transmembrane; Transmembrane helix; Transport; Vacuole.
FT   CHAIN           1..407
FT                   /note="P2X receptor D"
FT                   /id="PRO_0000390414"
FT   TOPO_DOM        1..22
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        23..43
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        44..300
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        301..321
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        322..407
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   REGION          283..296
FT                   /note="Pore-forming motif"
FT                   /evidence="ECO:0000255"
FT   REGION          371..394
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   407 AA;  46362 MW;  650A51857F6E0D2E CRC64;
     MDWDNIFSYN TAKIVTIKDR RLGGLHIIFM VLIIVYIVIY STIYKKGYLL TETPVGSIRA
     SLLAPNEFKD DSNFKYCDDN LIEYNFTKLE CDYYDEAFVS FPVGDDVSFA VTTRVKTLDQ
     VLNCSSKNPK CKYTTVSTRN VYVSDIEDFT ILIDHTMFAP SSLIQYNSKQ LKGYILDNDN
     NEIQINETIN TVGIPGKPDI LTIGKLLQLA NIDLDGASSV NSTNSVRYDG VVALVFITYS
     NTFSYNTNNF KYVYSIQKVE DTEYGVPEAV ILDNVSSRMY YNRHGIRLIF IQNGEIGSFN
     FQALLLTFVS GLGLLAISTV LVDQLAIRFL PERKTYSSHK FQITHGFSES RNKLRISQNE
     KDPLLLVETT KNNENNNNND DYNDDDNEIF DDNNNGYQNI QNNNIIL