P2Y13_HUMAN
ID P2Y13_HUMAN Reviewed; 354 AA.
AC Q9BPV8; B2R827; Q05C50; Q6DKN4; Q8IUT5; Q8TDU7; Q9BY61;
DT 10-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT 08-FEB-2011, sequence version 3.
DT 03-AUG-2022, entry version 175.
DE RecName: Full=P2Y purinoceptor 13;
DE Short=P2Y13;
DE AltName: Full=G-protein coupled receptor 86;
DE AltName: Full=G-protein coupled receptor 94;
GN Name=P2RY13; Synonyms=GPR86, GPR94; ORFNames=FKSG77;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING (ISOFORM 2), AND
RP VARIANT MET-179.
RX PubMed=11574155; DOI=10.1016/s0378-1119(01)00651-5;
RA Lee D.K., Nguyen T., Lynch K.R., Cheng R., Vanti W.B., Arkhitko O.,
RA Lewis T., Evans J.F., George S.R., O'Dowd B.F.;
RT "Discovery and mapping of ten novel G protein-coupled receptor genes.";
RL Gene 275:83-91(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, AND VARIANT MET-179.
RC TISSUE=Spleen;
RX PubMed=11546776; DOI=10.1074/jbc.m105912200;
RA Communi D., Gonzalez N.S., Detheux M., Brezillon S., Lannoy V.,
RA Parmentier M., Boeynaems J.-M.;
RT "Identification of a novel human ADP receptor coupled to Gi.";
RL J. Biol. Chem. 276:41479-41485(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), AND VARIANT MET-179.
RC TISSUE=Amygdala, Caudate nucleus, Fetal brain, and Placenta;
RX PubMed=11273702; DOI=10.1006/jmbi.2001.4520;
RA Wittenberger T., Schaller H.C., Hellebrand S.;
RT "An expressed sequence tag (EST) data mining strategy succeeding in the
RT discovery of new G-protein coupled receptors.";
RL J. Mol. Biol. 307:799-813(2001).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), AND VARIANT MET-179.
RC TISSUE=Heart;
RA Wang Y.-G., Gong L.;
RT "Molecular cloning of FKSG77, a novel gene encoding a putative G-protein-
RT coupled receptor.";
RL Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12044878; DOI=10.1016/s0014-5793(02)02775-8;
RA Takeda S., Kadowaki S., Haga T., Takaesu H., Mitaku S.;
RT "Identification of G protein-coupled receptor genes from the human genome
RT sequence.";
RL FEBS Lett. 520:97-101(2002).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANT MET-179.
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16641997; DOI=10.1038/nature04728;
RA Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J.,
RA Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P.,
RA Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A.,
RA Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L.,
RA Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G.,
RA Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W.,
RA Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M.,
RA Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P.,
RA Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H.,
RA Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J.,
RA Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W.,
RA Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B.,
RA Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O.,
RA Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B.,
RA Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H.,
RA Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J.,
RA Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X.,
RA Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R.,
RA Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.;
RT "The DNA sequence, annotation and analysis of human chromosome 3.";
RL Nature 440:1194-1198(2006).
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND VARIANT MET-179.
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [9]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANT MET-179.
RC TISSUE=Brain, and Placenta;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [10]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 172-354, AND VARIANT MET-179.
RA Zhang W., Wan T., Cao X.;
RT "Molecular cloning of a probable G protein-coupled receptor with three
RT transmembrane domains.";
RL Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Receptor for ADP. Coupled to G(i)-proteins. May play a role
CC in hematopoiesis and the immune system. {ECO:0000269|PubMed:11546776}.
CC -!- INTERACTION:
CC Q9BPV8; P48165: GJA8; NbExp=3; IntAct=EBI-17459480, EBI-17458373;
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9BPV8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9BPV8-2; Sequence=VSP_036226;
CC -!- TISSUE SPECIFICITY: Strong expression in spleen and adult brain. Lower
CC expression in placenta, lung, liver, spinal cord, thymus, small
CC intestine, uterus, stomach, testis, fetal brain, and adrenal gland. Not
CC detected in pancreas, heart, kidney, skeletal muscle, ovary or fetal
CC aorta. Clearly detected in lymph node and bone marrow, weakly detected
CC in peripheral blood mononuclear cells (PBMC) and in peripheral blood
CC leukocytes (PBL), but not detected in polymorphonuclear cells (PMN). In
CC the brain, detected in all brain regions examined.
CC -!- MISCELLANEOUS: Stimulation by ADP in stably transfected CHO cells
CC resulted in inhibition of adenylyl cyclase and the phosphorylation of
CC the MAP kinases MAPK3 and MAPK1 in a pertussis toxin-sensitive way.
CC Inhibition of adenylyl cyclase and phosphorylation of the MAP kinases
CC are transduction mechanisms that involve G(i) proteins.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC {ECO:0000255|PROSITE-ProRule:PRU00521}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH29363.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Potential poly-A sequence.; Evidence={ECO:0000305};
CC Sequence=AAH41116.2; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAG36024.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AF411113; AAL26484.1; -; Genomic_DNA.
DR EMBL; AF406692; AAL01038.1; -; mRNA.
DR EMBL; AF295368; AAK01864.1; -; mRNA.
DR EMBL; AF345565; AAK29068.1; -; mRNA.
DR EMBL; AB083597; BAB89310.1; -; Genomic_DNA.
DR EMBL; AK313208; BAG36024.1; ALT_INIT; mRNA.
DR EMBL; AC024886; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471052; EAW78805.1; -; Genomic_DNA.
DR EMBL; BC029363; AAH29363.1; ALT_SEQ; mRNA.
DR EMBL; BC041116; AAH41116.2; ALT_INIT; mRNA.
DR EMBL; BC066887; AAH66887.1; -; mRNA.
DR EMBL; AF178982; AAK18752.1; -; mRNA.
DR CCDS; CCDS3158.2; -. [Q9BPV8-1]
DR RefSeq; NP_795713.2; NM_176894.2. [Q9BPV8-1]
DR AlphaFoldDB; Q9BPV8; -.
DR SMR; Q9BPV8; -.
DR BioGRID; 119802; 11.
DR IntAct; Q9BPV8; 1.
DR STRING; 9606.ENSP00000320376; -.
DR BindingDB; Q9BPV8; -.
DR ChEMBL; CHEMBL3321651; -.
DR DrugBank; DB01069; Promethazine.
DR DrugCentral; Q9BPV8; -.
DR GuidetoPHARMACOLOGY; 329; -.
DR GlyGen; Q9BPV8; 3 sites.
DR iPTMnet; Q9BPV8; -.
DR PhosphoSitePlus; Q9BPV8; -.
DR BioMuta; P2RY13; -.
DR DMDM; 322510112; -.
DR EPD; Q9BPV8; -.
DR MassIVE; Q9BPV8; -.
DR PaxDb; Q9BPV8; -.
DR PeptideAtlas; Q9BPV8; -.
DR PRIDE; Q9BPV8; -.
DR ProteomicsDB; 78575; -. [Q9BPV8-1]
DR ProteomicsDB; 78576; -. [Q9BPV8-2]
DR Antibodypedia; 33610; 373 antibodies from 30 providers.
DR DNASU; 53829; -.
DR Ensembl; ENST00000325602.6; ENSP00000320376.5; ENSG00000181631.7. [Q9BPV8-1]
DR GeneID; 53829; -.
DR KEGG; hsa:53829; -.
DR MANE-Select; ENST00000325602.6; ENSP00000320376.5; NM_176894.3; NP_795713.2.
DR UCSC; uc003eyv.3; human. [Q9BPV8-1]
DR CTD; 53829; -.
DR DisGeNET; 53829; -.
DR GeneCards; P2RY13; -.
DR HGNC; HGNC:4537; P2RY13.
DR HPA; ENSG00000181631; Tissue enhanced (bone marrow, lymphoid tissue).
DR MIM; 606380; gene.
DR neXtProt; NX_Q9BPV8; -.
DR OpenTargets; ENSG00000181631; -.
DR VEuPathDB; HostDB:ENSG00000181631; -.
DR eggNOG; ENOG502QUS2; Eukaryota.
DR GeneTree; ENSGT01050000244845; -.
DR HOGENOM; CLU_009579_8_2_1; -.
DR InParanoid; Q9BPV8; -.
DR OMA; KIIRPFG; -.
DR OrthoDB; 971778at2759; -.
DR PhylomeDB; Q9BPV8; -.
DR TreeFam; TF330969; -.
DR PathwayCommons; Q9BPV8; -.
DR Reactome; R-HSA-417957; P2Y receptors.
DR Reactome; R-HSA-418594; G alpha (i) signalling events.
DR SignaLink; Q9BPV8; -.
DR SIGNOR; Q9BPV8; -.
DR BioGRID-ORCS; 53829; 11 hits in 1070 CRISPR screens.
DR GeneWiki; P2RY13; -.
DR GenomeRNAi; 53829; -.
DR Pharos; Q9BPV8; Tchem.
DR PRO; PR:Q9BPV8; -.
DR Proteomes; UP000005640; Chromosome 3.
DR RNAct; Q9BPV8; protein.
DR Bgee; ENSG00000181631; Expressed in monocyte and 124 other tissues.
DR Genevisible; Q9BPV8; HS.
DR GO; GO:0005783; C:endoplasmic reticulum; IEA:Ensembl.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; TAS:Reactome.
DR GO; GO:0045028; F:G protein-coupled purinergic nucleotide receptor activity; IBA:GO_Central.
DR GO; GO:0071407; P:cellular response to organic cyclic compound; IEA:Ensembl.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0007194; P:negative regulation of adenylate cyclase activity; IEA:InterPro.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR008109; P2Y13_rcpt.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PRINTS; PR01735; P2Y13PRNCPTR.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell membrane; Disulfide bond;
KW G-protein coupled receptor; Glycoprotein; Membrane; Receptor;
KW Reference proteome; Transducer; Transmembrane; Transmembrane helix.
FT CHAIN 1..354
FT /note="P2Y purinoceptor 13"
FT /id="PRO_0000070040"
FT TOPO_DOM 1..49
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 50..70
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 71..77
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 78..98
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 99..117
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 118..138
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 139..161
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 162..182
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 183..211
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 212..232
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 233..252
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 253..273
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 274..300
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 301..321
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 322..333
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 335..354
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 23
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 31
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 285
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 116..194
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
FT VAR_SEQ 1..21
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:11273702,
FT ECO:0000303|PubMed:11546776, ECO:0000303|Ref.4"
FT /id="VSP_036226"
FT VARIANT 179
FT /note="T -> M (in dbSNP:rs1466684)"
FT /evidence="ECO:0000269|PubMed:11273702,
FT ECO:0000269|PubMed:11546776, ECO:0000269|PubMed:11574155,
FT ECO:0000269|PubMed:14702039, ECO:0000269|PubMed:15489334,
FT ECO:0000269|Ref.10, ECO:0000269|Ref.4, ECO:0000269|Ref.8"
FT /id="VAR_028299"
SQ SEQUENCE 354 AA; 40789 MW; A2CE5F8ED84C4CAF CRC64;
MTAAIRRQRE LSILPKVTLE AMNTTVMQGF NRSERCPRDT RIVQLVFPAL YTVVFLTGIL
LNTLALWVFV HIPSSSTFII YLKNTLVADL IMTLMLPFKI LSDSHLAPWQ LRAFVCRFSS
VIFYETMYVG IVLLGLIAFD RFLKIIRPLR NIFLKKPVFA KTVSIFIWFF LFFISLPNTI
LSNKEATPSS VKKCASLKGP LGLKWHQMVN NICQFIFWTV FILMLVFYVV IAKKVYDSYR
KSKSKDRKNN KKLEGKVFVV VAVFFVCFAP FHFARVPYTH SQTNNKTDCR LQNQLFIAKE
TTLFLAATNI CMDPLIYIFL CKKFTEKLPC MQGRKTTASS QENHSSQTDN ITLG
