ID   P30_ASFB7               Reviewed;         204 AA.
AC   P34204;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   25-NOV-2008, sequence version 3.
DT   03-AUG-2022, entry version 69.
DE   RecName: Full=Phosphoprotein p30;
DE            Short=p30;
DE   AltName: Full=Phosphoprotein p32;
DE            Short=p32;
GN   OrderedLocusNames=Ba71V-93; ORFNames=CP204L;
OS   African swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V)
OS   (ASFV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Asfuvirales; Asfarviridae; Asfivirus.
OX   NCBI_TaxID=10498;
OH   NCBI_TaxID=6937; Ornithodoros (relapsing fever ticks).
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], PHOSPHORYLATION, AND SUBCELLULAR
RP   LOCATION.
RX   PubMed=8474154; DOI=10.1128/jvi.67.5.2475-2485.1993;
RA   Prados F.J., Vinuela E., Alcami A.;
RT   "Sequence and characterization of the major early phosphoprotein p32 of
RT   African swine fever virus.";
RL   J. Virol. 67:2475-2485(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=11831707; DOI=10.1006/viro.1995.1149;
RA   Yanez R.J., Rodriguez J.M., Nogal M.L., Yuste L., Enriquez C.,
RA   Rodriguez J.F., Vinuela E.;
RT   "Analysis of the complete nucleotide sequence of African swine fever
RT   virus.";
RL   Virology 208:249-278(1995).
RN   [3]
RP   FUNCTION.
RX   PubMed=9568043; DOI=10.1006/viro.1998.9068;
RA   Gomez-Puertas P., Rodriguez F., Oviedo J.M., Brun A., Alonso C.,
RA   Escribano J.M.;
RT   "The African swine fever virus proteins p54 and p30 are involved in two
RT   distinct steps of virus attachment and both contribute to the antibody-
RT   mediated protective immune response.";
RL   Virology 243:461-471(1998).
RN   [4]
RP   INTERACTION WITH HUMAN HNRNPK, FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=18775702; DOI=10.1016/j.febslet.2008.08.031;
RA   Hernaez B., Escribano J.M., Alonso C.;
RT   "African swine fever virus protein p30 interaction with heterogeneous
RT   nuclear ribonucleoprotein K (hnRNP-K) during infection.";
RL   FEBS Lett. 582:3275-3280(2008).
RN   [5]
RP   SUBCELLULAR LOCATION.
RX   PubMed=30185597; DOI=10.1128/jvi.01293-18;
RA   Alejo A., Matamoros T., Guerra M., Andres G.;
RT   "A Proteomic Atlas of the African Swine Fever Virus Particle.";
RL   J. Virol. 92:0-0(2018).
RN   [6]
RP   INDUCTION, AND ALTERNATIVE INITIATION.
RX   PubMed=32075923; DOI=10.1128/jvi.00119-20;
RA   Cackett G., Matelska D., Sykora M., Portugal R., Malecki M., Baehler J.,
RA   Dixon L., Werner F.;
RT   "The African Swine Fever Virus Transcriptome.";
RL   J. Virol. 94:0-0(2020).
CC   -!- FUNCTION: Modifies the subcellular distribution of heterogeneous
CC       nuclear ribonucleoprotein K (HNRNPK) and may contribute to modulate
CC       HNRNPK functions related to processing and export of mRNAs during ASFV
CC       infection (PubMed:18775702). Necessary for virus internalization
CC       (PubMed:9568043). {ECO:0000269|PubMed:18775702,
CC       ECO:0000269|PubMed:9568043}.
CC   -!- SUBUNIT: Oligomer. Interacts with host HNRNPK.
CC       {ECO:0000269|PubMed:18775702}.
CC   -!- SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000269|PubMed:8474154}. Host
CC       nucleus {ECO:0000269|PubMed:18775702, ECO:0000269|PubMed:8474154}.
CC       Virion {ECO:0000269|PubMed:30185597}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative initiation; Named isoforms=2;
CC       Name=1;
CC         IsoId=P34204-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=P34204-2; Sequence=VSP_061331;
CC   -!- INDUCTION: Expressed in the early phase of the viral replicative cycle.
CC       {ECO:0000269|PubMed:32075923}.
CC   -!- PTM: Phosphorylated on serine residues in the 115 N-terminal amino
CC       acids. {ECO:0000269|PubMed:8474154}.
CC   -!- SIMILARITY: Belongs to the asfivirus phosphoprotein p30 family.
CC       {ECO:0000305}.
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DR   EMBL; M96354; AAA02818.1; -; Genomic_DNA.
DR   EMBL; U18466; AAA65322.1; -; Genomic_DNA.
DR   RefSeq; NP_042786.1; NC_001659.2.
DR   SMR; P34204; -.
DR   GeneID; 22220322; -.
DR   KEGG; vg:22220322; -.
DR   Proteomes; UP000000624; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0075509; P:endocytosis involved in viral entry into host cell; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Alternative initiation; Early protein; Host cytoplasm; Host nucleus;
KW   Host-virus interaction; Phosphoprotein; Reference proteome;
KW   Viral penetration into host cytoplasm; Virion; Virus endocytosis by host;
KW   Virus entry into host cell.
FT   CHAIN           1..204
FT                   /note="Phosphoprotein p30"
FT                   /id="PRO_0000221960"
FT   VAR_SEQ         1..8
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000269|PubMed:32075923"
FT                   /id="VSP_061331"
SQ   SEQUENCE   204 AA;  23555 MW;  E3D441D48E6BF5A3 CRC64;
     MDFILNISMK MEVIFKTDLR SSSQVVFHAG SLYNWFSVEI INSGRIVTTA IKTLLSTVKY
     DIVKSAHIYA GQGYTEHQAQ EEWNMILHVL FEEETESSAS SESIHEKNDN ETNECTSSFE
     TLFEQEPSSE EPKDSKLYML AQKTVQHIEQ YGKAPDFNKV IRAHNFIQTI HGTPLKEEEK
     EVVRLMVIKL LKKNKLLSHL HLMF