ID   P32_MYCGA               Reviewed;         294 AA.
AC   Q49378;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   15-AUG-2003, sequence version 2.
DT   03-AUG-2022, entry version 97.
DE   RecName: Full=P32 adhesin;
DE   AltName: Full=Cytadhesin P32;
GN   Name=mgc2; OrderedLocusNames=MYCGA1790; ORFNames=MGA_0932;
OS   Mycoplasma gallisepticum (strain R(low / passage 15 / clone 2))
OS   (Mycoplasmoides gallisepticum).
OC   Bacteria; Tenericutes; Mollicutes; Mycoplasmataceae; Mycoplasma.
OX   NCBI_TaxID=710127;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=S6;
RX   PubMed=9632619; DOI=10.1128/iai.66.7.3436-3442.1998;
RA   Hnatow L.L., Keeler C.L. Jr., Tessmer L.L., Czymmek K., Dohms J.E.;
RT   "Characterization of MGC2, a Mycoplasma gallisepticum cytadhesin with
RT   homology to the Mycoplasma pneumoniae 30-kilodalton protein P30 and
RT   Mycoplasma genitalium P32.";
RL   Infect. Immun. 66:3436-3442(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=R(low / passage 15 / clone 2);
RX   PubMed=12949158; DOI=10.1099/mic.0.26427-0;
RA   Papazisi L., Gorton T.S., Kutish G., Markham P.F., Browning G.F.,
RA   Nguyen D.K., Swartzell S., Madan A., Mahairas G., Geary S.J.;
RT   "The complete genome sequence of the avian pathogen Mycoplasma
RT   gallisepticum strain R(low).";
RL   Microbiology 149:2307-2316(2003).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 260-294.
RC   STRAIN=S6;
RX   PubMed=8613358; DOI=10.1128/iai.64.5.1541-1547.1996;
RA   Keeler C.L. Jr., Hnatow L.L., Whetzel P.L., Dohms J.E.;
RT   "Cloning and characterization of a putative cytadhesin gene (mgc1) from
RT   Mycoplasma gallisepticum.";
RL   Infect. Immun. 64:1541-1547(1996).
CC   -!- FUNCTION: Adhesin necessary for successful cytadherence and virulence.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell projection, attachment organelle membrane
CC       {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}. Note=Integral
CC       and surface exposed membrane protein that localizes to the membrane at
CC       the attachment organelle. {ECO:0000250}.
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DR   EMBL; U34842; AAB02986.1; -; Genomic_DNA.
DR   EMBL; AE015450; AAP56529.2; -; Genomic_DNA.
DR   PIR; T18345; T18345.
DR   RefSeq; WP_011113411.1; NC_004829.2.
DR   AlphaFoldDB; Q49378; -.
DR   SMR; Q49378; -.
DR   PRIDE; Q49378; -.
DR   KEGG; mga:MGA_0932; -.
DR   PATRIC; fig|233150.7.peg.196; -.
DR   HOGENOM; CLU_946011_0_0_14; -.
DR   OMA; NNFNPRM; -.
DR   OrthoDB; 2153279at2; -.
DR   Proteomes; UP000001418; Chromosome.
DR   GO; GO:0033111; C:attachment organelle membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0042995; C:cell projection; IEA:UniProtKB-KW.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-KW.
DR   GO; GO:0020035; P:adhesion of symbiont to microvasculature; IEA:UniProtKB-KW.
DR   InterPro; IPR009896; Cytadhesin_P30/P32.
DR   Pfam; PF07271; Cytadhesin_P30; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Cell projection; Cytadherence; Membrane; Reference proteome;
KW   Repeat; Transmembrane; Transmembrane helix; Virulence.
FT   CHAIN           1..294
FT                   /note="P32 adhesin"
FT                   /id="PRO_0000058132"
FT   TRANSMEM        11..31
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        66..86
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REPEAT          172..193
FT                   /note="1"
FT   REPEAT          194..214
FT                   /note="2"
FT   REGION          172..214
FT                   /note="2 X 22 AA repeats"
FT   REGION          234..294
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        21
FT                   /note="V -> A (in Ref. 1; AAB02986)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        125
FT                   /note="T -> TAAVPT (in Ref. 1)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        152
FT                   /note="P -> PGFNQP (in Ref. 1)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        170
FT                   /note="C -> G (in Ref. 1; AAB02986)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        176
FT                   /note="Q -> P (in Ref. 1; AAB02986)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        252
FT                   /note="G -> V (in Ref. 1; AAB02986)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        260
FT                   /note="T -> A (in Ref. 1 and 3)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        279
FT                   /note="H -> Y (in Ref. 1 and 3)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   294 AA;  31784 MW;  D76AECC6B5A4E32E CRC64;
     MFSLKKLKSK LVGVSFVFSG VIALGTGVGL TSEHKYEHSP TLVLHEGETN SVGPRKITSE
     PWFYPVVGAG AGLIVVSLLL GLGIGIPIAK KKERMMIQER EEHQKMVESL GIIEEQNKTE
     AIEPTEEVNT QEPTQPAGVN VANNPQMGIN QPQINPQFGP NPQQRINPQC FGGPMQPNQM
     GMRPGFNQMP PQMGGMPPNQ MGMRPGFNQM PPQMGGMPPR PNFPNQMPNM NQPRPGFRPQ
     PGGGVPMGNK AGGGFNHPGT PMGPNRMNFP NQGMNQPPHM AGPRAGFPPQ NGPR