P33MX_HUMAN
ID P33MX_HUMAN Reviewed; 305 AA.
AC Q96A73; B2RD69; B8K1S6; Q6IA24; Q8NDU3; Q9BRE5; Q9H7D5; Q9ULM9;
DT 23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Putative monooxygenase p33MONOX;
DE EC=1.-.-.-;
DE AltName: Full=Brain-derived rescue factor p60MONOX;
DE AltName: Full=Flavin monooxygenase motif-containing protein of 33 kDa;
GN Name=KIAA1191; Synonyms=P33MONOX;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RA Heese K.;
RT "Brain-derived rescue factor p60MONOX.";
RL Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Brain, and Small intestine;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15372022; DOI=10.1038/nature02919;
RA Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S.,
RA Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M.,
RA She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S.,
RA Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M.,
RA Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M.,
RA Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T.,
RA Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A.,
RA Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R.,
RA Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L.,
RA Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N.,
RA Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J.,
RA Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A.,
RA Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.;
RT "The DNA sequence and comparative analysis of human chromosome 5.";
RL Nature 431:268-274(2004).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
RC TISSUE=Brain, Muscle, and Ovary;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 206-305 (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=10574461; DOI=10.1093/dnares/6.5.329;
RA Hirosawa M., Nagase T., Ishikawa K., Kikuno R., Nomura N., Ohara O.;
RT "Characterization of cDNA clones selected by the GeneMark analysis from
RT size-fractionated cDNA libraries from human brain.";
RL DNA Res. 6:329-336(1999).
RN [9]
RP TISSUE SPECIFICITY.
RX PubMed=17032350; DOI=10.1111/j.1365-2362.2006.01722.x;
RA Yokota T., Mishra M., Akatsu H., Tani Y., Miyauchi T., Yamamoto T.,
RA Kosaka K., Nagai Y., Sawada T., Heese K.;
RT "Brain site-specific gene expression analysis in Alzheimer's disease
RT patients.";
RL Eur. J. Clin. Invest. 36:820-830(2006).
RN [10]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19413330; DOI=10.1021/ac9004309;
RA Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.;
RT "Lys-N and trypsin cover complementary parts of the phosphoproteome in a
RT refined SCX-based approach.";
RL Anal. Chem. 81:4493-4501(2009).
RN [11]
RP INTERACTION WITH NELFB; NOL12 AND PRNP.
RX PubMed=21153684; DOI=10.1007/s11010-010-0690-4;
RA Mishra M., Inoue N., Heese K.;
RT "Characterizing the novel protein p33MONOX.";
RL Mol. Cell. Biochem. 350:127-134(2011).
RN [12]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-182 AND SER-183, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma, and Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
RN [13]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-183, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA Ye M., Zou H.;
RT "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT phosphoproteome.";
RL J. Proteomics 96:253-262(2014).
CC -!- FUNCTION: Potential NADPH-dependent oxidoreductase. May be involved in
CC the regulation of neuronal survival, differentiation and axonal
CC outgrowth.
CC -!- SUBUNIT: Interacts with NELFB, NOL12 and PRNP.
CC {ECO:0000269|PubMed:21153684}.
CC -!- INTERACTION:
CC Q96A73; Q9NUX5: POT1; NbExp=2; IntAct=EBI-725897, EBI-752420;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q96A73-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q96A73-2; Sequence=VSP_028801;
CC Name=3;
CC IsoId=Q96A73-3; Sequence=VSP_028800;
CC -!- TISSUE SPECIFICITY: Down-regulated in the occipital lobe of an early
CC stage Alzheimer disease patients. {ECO:0000269|PubMed:17032350}.
CC -!- SIMILARITY: Belongs to the P33MONOX family. {ECO:0000305}.
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DR EMBL; DQ073392; AAY82896.1; -; mRNA.
DR EMBL; AK024675; BAB14957.1; -; mRNA.
DR EMBL; AK315428; BAG37816.1; -; mRNA.
DR EMBL; CR457331; CAG33612.1; -; mRNA.
DR EMBL; AL390217; CAB99231.1; -; mRNA.
DR EMBL; BX648859; CAH56134.1; -; mRNA.
DR EMBL; AC138956; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471195; EAW85087.1; -; Genomic_DNA.
DR EMBL; BC006316; AAH06316.1; -; mRNA.
DR EMBL; BC010448; AAH10448.1; -; mRNA.
DR EMBL; BC012848; AAH12848.1; -; mRNA.
DR EMBL; BC017097; AAH17097.1; -; mRNA.
DR EMBL; AB033017; BAA86505.1; -; mRNA.
DR CCDS; CCDS43402.1; -. [Q96A73-2]
DR CCDS; CCDS4399.1; -. [Q96A73-1]
DR RefSeq; NP_001073152.1; NM_001079684.2. [Q96A73-2]
DR RefSeq; NP_001073153.1; NM_001079685.2. [Q96A73-1]
DR RefSeq; NP_001274264.1; NM_001287335.1.
DR RefSeq; NP_001274265.1; NM_001287336.1. [Q96A73-2]
DR RefSeq; NP_065177.2; NM_020444.4. [Q96A73-1]
DR RefSeq; XP_005265998.1; XM_005265941.1.
DR RefSeq; XP_005266003.1; XM_005265946.4. [Q96A73-2]
DR AlphaFoldDB; Q96A73; -.
DR SMR; Q96A73; -.
DR BioGRID; 121428; 43.
DR IntAct; Q96A73; 24.
DR MINT; Q96A73; -.
DR STRING; 9606.ENSP00000298569; -.
DR iPTMnet; Q96A73; -.
DR PhosphoSitePlus; Q96A73; -.
DR BioMuta; KIAA1191; -.
DR DMDM; 74731096; -.
DR EPD; Q96A73; -.
DR jPOST; Q96A73; -.
DR MassIVE; Q96A73; -.
DR MaxQB; Q96A73; -.
DR PaxDb; Q96A73; -.
DR PeptideAtlas; Q96A73; -.
DR PRIDE; Q96A73; -.
DR ProteomicsDB; 75930; -. [Q96A73-1]
DR ProteomicsDB; 75931; -. [Q96A73-2]
DR ProteomicsDB; 75932; -. [Q96A73-3]
DR Antibodypedia; 45989; 99 antibodies from 20 providers.
DR DNASU; 57179; -.
DR Ensembl; ENST00000298569.9; ENSP00000298569.4; ENSG00000122203.15. [Q96A73-1]
DR Ensembl; ENST00000393725.6; ENSP00000377326.2; ENSG00000122203.15. [Q96A73-2]
DR Ensembl; ENST00000510164.5; ENSP00000421061.1; ENSG00000122203.15. [Q96A73-1]
DR Ensembl; ENST00000614420.4; ENSP00000479627.1; ENSG00000122203.15. [Q96A73-2]
DR GeneID; 57179; -.
DR KEGG; hsa:57179; -.
DR MANE-Select; ENST00000298569.9; ENSP00000298569.4; NM_020444.5; NP_065177.2.
DR UCSC; uc003mdw.5; human. [Q96A73-1]
DR CTD; 57179; -.
DR GeneCards; KIAA1191; -.
DR HGNC; HGNC:29209; KIAA1191.
DR HPA; ENSG00000122203; Low tissue specificity.
DR neXtProt; NX_Q96A73; -.
DR OpenTargets; ENSG00000122203; -.
DR PharmGKB; PA143485516; -.
DR VEuPathDB; HostDB:ENSG00000122203; -.
DR eggNOG; ENOG502QRB0; Eukaryota.
DR GeneTree; ENSGT00390000000537; -.
DR HOGENOM; CLU_079377_0_0_1; -.
DR InParanoid; Q96A73; -.
DR OMA; SPRSHNI; -.
DR PhylomeDB; Q96A73; -.
DR TreeFam; TF332226; -.
DR PathwayCommons; Q96A73; -.
DR SignaLink; Q96A73; -.
DR BioGRID-ORCS; 57179; 17 hits in 1074 CRISPR screens.
DR ChiTaRS; KIAA1191; human.
DR GenomeRNAi; 57179; -.
DR Pharos; Q96A73; Tdark.
DR PRO; PR:Q96A73; -.
DR Proteomes; UP000005640; Chromosome 5.
DR RNAct; Q96A73; protein.
DR Bgee; ENSG00000122203; Expressed in kidney epithelium and 188 other tissues.
DR ExpressionAtlas; Q96A73; baseline and differential.
DR Genevisible; Q96A73; HS.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR InterPro; IPR026759; P33MONOX.
DR PANTHER; PTHR28342; PTHR28342; 1.
DR Pfam; PF15302; P33MONOX; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; NADP; Oxidoreductase; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..305
FT /note="Putative monooxygenase p33MONOX"
FT /id="PRO_0000307730"
FT REGION 37..56
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 158..236
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 259..305
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 67..77
FT /note="Flavin-containing monooxygenase motif"
FT COMPBIAS 164..213
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 277..291
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 44
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q9DBN4"
FT MOD_RES 175
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q9DBN4"
FT MOD_RES 182
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT MOD_RES 183
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163,
FT ECO:0007744|PubMed:24275569"
FT VAR_SEQ 1..207
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_028800"
FT VAR_SEQ 1..19
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:17974005"
FT /id="VSP_028801"
FT CONFLICT 56
FT /note="K -> R (in Ref. 2; BAB14957)"
FT /evidence="ECO:0000305"
FT CONFLICT 97
FT /note="V -> A (in Ref. 4; CAB99231)"
FT /evidence="ECO:0000305"
FT CONFLICT 225
FT /note="L -> P (in Ref. 3; CAG33612)"
FT /evidence="ECO:0000305"
FT CONFLICT 250
FT /note="P -> S (in Ref. 4; CAB99231)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 305 AA; 33247 MW; 0BC0519F401593CF CRC64;
MASRQPEVPA LEASAPLGKM SLPIGIYRRA VSYDDTLEDP APMTPPPSDM GSVPWKPVIP
ERKYQHLAKV EEGEASLPSP AMTLSSAIDS VDKVPVVKAK ATHVIMNSLI TKQTQESIQH
FERQAGLRDA GYTPHKGLTT EETKYLRVAE ALHKLKLQSG EVTKEERQPA SAQSTPSTTP
HSSPKQRPRG WFTSGSSTAL PGPNPSTMDS GSGDKDRNLS DKWSLFGPRS LQKYDSGSFA
TQAYRGAQKP SPLELIRAQA NRMAEDPAAL KPPKMDIPVM EGKKQPPRAH NLKPRDLNVL
TPTGF
