P33MX_MOUSE
ID P33MX_MOUSE Reviewed; 303 AA.
AC Q9DBN4; Q3TJ26; Q3UMA7; Q69ZP2; Q8C1J8; Q9D4T9;
DT 23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Putative monooxygenase p33MONOX;
DE EC=1.-.-.-;
GN Name=P33monox; Synonyms=Kiaa1191;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX PubMed=15368895; DOI=10.1093/dnares/11.3.205;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S.,
RA Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H.,
RA Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: IV.
RT The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 11:205-218(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
RC STRAIN=C57BL/6J;
RC TISSUE=Amnion, Head, Liver, Mammary gland, Placenta, Skin, and Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J, and FVB/N; TISSUE=Brain, Kidney, and Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT "Large-scale phosphorylation analysis of mouse liver.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19144319; DOI=10.1016/j.immuni.2008.11.006;
RA Trost M., English L., Lemieux S., Courcelles M., Desjardins M.,
RA Thibault P.;
RT "The phagosomal proteome in interferon-gamma-activated macrophages.";
RL Immunity 30:143-154(2009).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-44; THR-175 AND SER-183, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Heart, Kidney, Lung, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [7]
RP TISSUE SPECIFICITY, AND SUBCELLULAR LOCATION.
RX PubMed=21153684; DOI=10.1007/s11010-010-0690-4;
RA Mishra M., Inoue N., Heese K.;
RT "Characterizing the novel protein p33MONOX.";
RL Mol. Cell. Biochem. 350:127-134(2011).
CC -!- FUNCTION: Potential NADPH-dependent oxidoreductase. May be involved in
CC the regulation of neuronal survival, differentiation and axonal
CC outgrowth (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Interacts with NELFB, NOL12 and PRNP.
CC {ECO:0000250|UniProtKB:Q96A73}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:21153684}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q9DBN4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9DBN4-2; Sequence=VSP_028802, VSP_028803;
CC Name=3;
CC IsoId=Q9DBN4-3; Sequence=VSP_028804, VSP_028805;
CC -!- TISSUE SPECIFICITY: Expressed in neuronal pyramidal cells of the
CC hippocampus and in the neurons of the cortex.
CC {ECO:0000269|PubMed:21153684}.
CC -!- SIMILARITY: Belongs to the P33MONOX family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAD32404.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC -!- SEQUENCE CAUTION: [Isoform 2]:
CC Sequence=BAC25449.1; Type=Frameshift; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK173126; BAD32404.1; ALT_INIT; mRNA.
DR EMBL; AK004845; BAB23611.1; -; mRNA.
DR EMBL; AK014792; BAC25449.1; ALT_FRAME; mRNA.
DR EMBL; AK016176; BAB30137.1; -; mRNA.
DR EMBL; AK028697; BAC26072.1; -; mRNA.
DR EMBL; AK145025; BAE26191.1; -; mRNA.
DR EMBL; AK159945; BAE35501.1; -; mRNA.
DR EMBL; AK167616; BAE39669.1; -; mRNA.
DR EMBL; AK169399; BAE41145.1; -; mRNA.
DR EMBL; BC033445; AAH33445.1; -; mRNA.
DR EMBL; BC039775; AAH39775.1; -; mRNA.
DR EMBL; BC043040; AAH43040.1; -; mRNA.
DR EMBL; BC054417; AAH54417.1; -; mRNA.
DR CCDS; CCDS26529.1; -. [Q9DBN4-1]
DR RefSeq; NP_001239574.1; NM_001252645.1.
DR RefSeq; NP_001239575.1; NM_001252646.1.
DR RefSeq; NP_001239576.1; NM_001252647.1.
DR RefSeq; NP_001239577.1; NM_001252648.1.
DR RefSeq; NP_598558.1; NM_133797.4. [Q9DBN4-1]
DR RefSeq; XP_006517541.1; XM_006517478.3. [Q9DBN4-1]
DR AlphaFoldDB; Q9DBN4; -.
DR SMR; Q9DBN4; -.
DR BioGRID; 220877; 3.
DR STRING; 10090.ENSMUSP00000026989; -.
DR iPTMnet; Q9DBN4; -.
DR PhosphoSitePlus; Q9DBN4; -.
DR EPD; Q9DBN4; -.
DR MaxQB; Q9DBN4; -.
DR PaxDb; Q9DBN4; -.
DR PeptideAtlas; Q9DBN4; -.
DR PRIDE; Q9DBN4; -.
DR ProteomicsDB; 294091; -. [Q9DBN4-2]
DR ProteomicsDB; 294092; -. [Q9DBN4-3]
DR Antibodypedia; 45989; 99 antibodies from 20 providers.
DR DNASU; 97820; -.
DR Ensembl; ENSMUST00000026989; ENSMUSP00000026989; ENSMUSG00000025871. [Q9DBN4-1]
DR GeneID; 97820; -.
DR KEGG; mmu:97820; -.
DR UCSC; uc007qof.3; mouse. [Q9DBN4-1]
DR MGI; MGI:1921162; 4833439L19Rik.
DR VEuPathDB; HostDB:ENSMUSG00000025871; -.
DR eggNOG; ENOG502QRB0; Eukaryota.
DR GeneTree; ENSGT00390000000537; -.
DR InParanoid; Q9DBN4; -.
DR OMA; SPRSHNI; -.
DR OrthoDB; 1021703at2759; -.
DR PhylomeDB; Q9DBN4; -.
DR TreeFam; TF332226; -.
DR BioGRID-ORCS; 97820; 3 hits in 73 CRISPR screens.
DR ChiTaRS; 4833439L19Rik; mouse.
DR PRO; PR:Q9DBN4; -.
DR Proteomes; UP000000589; Chromosome 13.
DR RNAct; Q9DBN4; protein.
DR Bgee; ENSMUSG00000025871; Expressed in right kidney and 268 other tissues.
DR ExpressionAtlas; Q9DBN4; baseline and differential.
DR Genevisible; Q9DBN4; MM.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR InterPro; IPR026759; P33MONOX.
DR PANTHER; PTHR28342; PTHR28342; 1.
DR Pfam; PF15302; P33MONOX; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; NADP; Oxidoreductase; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..303
FT /note="Putative monooxygenase p33MONOX"
FT /id="PRO_0000307731"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 37..56
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 66..96
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 156..233
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 260..283
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 67..77
FT /note="Flavin-containing monooxygenase motif"
FT COMPBIAS 76..90
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 169..203
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 44
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 175
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 183
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT VAR_SEQ 1..70
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_028802"
FT VAR_SEQ 71..112
FT /note="EEGAASVSSLAVTPSPATDSSDKAPVVKAKATHVIMSSLITK -> MGAFVA
FT LSRWLCSVQPSSLLGHLEPRSLFSAGSRAAGGGRAE (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_028803"
FT VAR_SEQ 152..164
FT /note="LHKLKLQSGETAK -> PHFPFKTASVIPF (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_028804"
FT VAR_SEQ 165..303
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_028805"
FT CONFLICT 13
FT /note="P -> H (in Ref. 2; BAE26191)"
FT /evidence="ECO:0000305"
FT CONFLICT 127..139
FT /note="LRDAGYTPHKGLT -> RRDSCITPQWGLS (in Ref. 2;
FT BAB30137)"
FT /evidence="ECO:0000305"
FT CONFLICT 143
FT /note="T -> A (in Ref. 2; BAE39669)"
FT /evidence="ECO:0000305"
FT CONFLICT 145
FT /note="Y -> L (in Ref. 2; BAB30137)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 303 AA; 32715 MW; 2203ADB0AC7EB153 CRC64;
MASRQPEVPA LAPSGPLGKM SLPIGMCRRA FSYDDALEDP APMTPPPSDM GSIPWKPVIP
ERKYQHLDKT EEGAASVSSL AVTPSPATDS SDKAPVVKAK ATHVIMSSLI TKQTQESIQR
FEQQAGLRDA GYTPHKGLTT EETKYLRVAE ALHKLKLQSG ETAKEEKHPA SAQSTPSSTP
HASPKQKSRG WFPSGSSTAL PAPNPHTMDP GSGNDRNSAD KWSLFGPRPL QKSDSGFAIQ
AYKGAPRPSP MEVMRAQATR VGEDPATFKP PKMDVPMVEG KKQPLRTHNL KPRDLNVLTP
TGF
