ID   P35_MALPE               Reviewed;         362 AA.
AC   P0DJ01; Q50367;
DT   21-SEP-2011, integrated into UniProtKB/Swiss-Prot.
DT   21-SEP-2011, sequence version 1.
DT   03-AUG-2022, entry version 18.
DE   RecName: Full=Lipoprotein p35;
DE   Flags: Precursor;
OS   Malacoplasma penetrans (Mycoplasma penetrans).
OC   Bacteria; Tenericutes; Mycoplasmoidales; Mycoplasmoidaceae; Malacoplasma.
OX   NCBI_TaxID=28227;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, BLOCKAGE OF N-TERMINUS, AND
RP   PROTEIN SEQUENCE OF 211-225.
RC   STRAIN=ATCC 55252 / DSM 22633 / GTU-54-6A1;
RX   PubMed=7649455; DOI=10.1111/j.1574-6968.1995.tb07737.x;
RA   Ferris S., Watson H.L., Neyrolles O., Montagnier L., Blanchard A.;
RT   "Characterization of a major Mycoplasma penetrans lipoprotein and of its
RT   gene.";
RL   FEMS Microbiol. Lett. 130:313-319(1995).
RN   [2]
RP   CHARACTERIZATION, FUNCTION, AND SUBCELLULAR LOCATION.
RC   STRAIN=ATCC 55252 / DSM 22633 / GTU-54-6A1;
RX   PubMed=10075417; DOI=10.1099/13500872-145-2-343;
RA   Neyrolles O., Eliane J.-P., Ferris S., da Cunha R.A.F., Prevost M.-C.,
RA   Bahraoui E., Blanchard A.;
RT   "Antigenic characterization and cytolocalization of P35, the major
RT   Mycoplasma penetrans antigen.";
RL   Microbiology 145:343-355(1999).
CC   -!- FUNCTION: Major M.penetrans antigen. {ECO:0000269|PubMed:10075417,
CC       ECO:0000269|PubMed:7649455}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|PROSITE-
CC       ProRule:PRU00303, ECO:0000269|PubMed:10075417}; Lipid-anchor
CC       {ECO:0000255|PROSITE-ProRule:PRU00303, ECO:0000269|PubMed:10075417};
CC       Extracellular side {ECO:0000269|PubMed:10075417}. Note=Distributed all
CC       over the plasma membrane.
CC   -!- PTM: The N-terminus is blocked. {ECO:0000305}.
CC   -!- MISCELLANEOUS: The dominant B-epitopes are found at the N- and C-
CC       terminal regions.
CC   -!- SIMILARITY: Belongs to the p35 lipoprotein family. {ECO:0000305}.
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DR   EMBL; L38250; AAC16392.1; -; Genomic_DNA.
DR   AlphaFoldDB; P0DJ01; -.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR011653; Lipoprotein_p35.
DR   Pfam; PF07668; MpPF1; 1.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Direct protein sequencing; Lipoprotein; Membrane; Palmitate;
KW   Signal.
FT   SIGNAL          1..30
FT                   /evidence="ECO:0000305"
FT   CHAIN           31..362
FT                   /note="Lipoprotein p35"
FT                   /id="PRO_0000412636"
FT   REGION          33..53
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   LIPID           31
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000305"
FT   LIPID           31
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   362 AA;  38384 MW;  6325A719B76F8837 CRC64;
     MKIKKIKLLK ALALTGAFGI VATVPVIVSS CSSTSENNGN GNGNGGTDGN TQQTEVTPAI
     KSEVSLTGAL SKIYDTKTGT DRETTSQLIV KDIKANPENY FTNGEALKDV IASATVTVDG
     GFTESTFTGE AYSVWSAKAD VKKGTYSQAS KQLDIKSIND LQTVLGDSAA IKGICDLIPN
     LKLNNGTDYK VTNNGLSLSE DLLHINVTAK DGQTDVSMDL AIPVSDLNLK IDGLKISVSG
     TGIKTSELTT NYKFNIGIDN TVKTLTPAAV TLAEADRTNA EKVLEKLGYA TVSGSTYTLD
     QDKLADALGL YNCKFEAVKS EKDSTNNNKY TVTLKATPND GYFWEDGTNG AKEEISFVAT
     FS