ID   P3A_PLRV1               Reviewed;          45 AA.
AC   P0DTK2;
DT   25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT   25-MAY-2022, sequence version 1.
DT   03-AUG-2022, entry version 2.
DE   RecName: Full=Movement protein P3a {ECO:0000303|PubMed:30373157};
DE   AltName: Full=Protein ORF3a;
GN   Name=ORF3a;
OS   Potato leafroll virus (strain Potato/Scotland/strain 1/1984) (PLrV).
OC   Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC   Sobelivirales; Solemoviridae; Polerovirus.
OX   NCBI_TaxID=12046;
OH   NCBI_TaxID=4113; Solanum tuberosum (Potato).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=2732710; DOI=10.1099/0022-1317-70-5-1037;
RA   Mayo M.A., Robinson D.J., Jolly C.A., Hyman L.;
RT   "Nucleotide sequence of potato leafroll luteovirus RNA.";
RL   J. Gen. Virol. 70:1037-1051(1989).
RN   [2]
RP   IDENTIFICATION.
RX   PubMed=25946037; DOI=10.1371/journal.ppat.1004868;
RA   Smirnova E., Firth A.E., Miller W.A., Scheidecker D., Brault V.,
RA   Reinbold C., Rakotondrafara A.M., Chung B.Y., Ziegler-Graff V.;
RT   "Discovery of a small non-AUG-initiated ORF in Poleroviruses and
RT   Luteoviruses that is required for long-distance movement.";
RL   PLoS Pathog. 11:E1004868-E1004868(2015).
RN   [3]
RP   PROTEIN SEQUENCE OF 37-45, FUNCTION, SUBUNIT, SUBCELLULAR LOCATION,
RP   INTERACTION WITH MOVEMENT PROTEIN P17, AND DOMAIN.
RX   PubMed=30373157; DOI=10.3390/v10110585;
RA   DeBlasio S.L., Xu Y., Johnson R.S., Rebelo A.R., MacCoss M.J., Gray S.M.,
RA   Heck M.;
RT   "The Interaction Dynamics of Two Potato Leafroll Virus Movement Proteins
RT   Affects Their Localization to the Outer Membranes of Mitochondria and
RT   Plastids.";
RL   Viruses 10:0-0(2018).
CC   -!- FUNCTION: Together with movement protein P17, plays an essential role
CC       in virus long distance movement. {ECO:0000269|PubMed:30373157}.
CC   -!- SUBUNIT: Homodimer (PubMed:30373157). Heterodimer with movement protein
CC       P17 (PubMed:30373157). {ECO:0000269|PubMed:30373157}.
CC   -!- SUBCELLULAR LOCATION: Host cell junction, host plasmodesma
CC       {ECO:0000250|UniProtKB:P0DJZ8}. Host Golgi apparatus
CC       {ECO:0000250|UniProtKB:P0DJZ8}. Host chloroplast envelope
CC       {ECO:0000305|PubMed:30373157}. Host mitochondrion outer membrane
CC       {ECO:0000305|PubMed:30373157}. Note=P3a directs P17 to the
CC       mitochondrial outer membrane while P17 regulates the localization of
CC       the P3a-P17 heterodimer to plastids. {ECO:0000269|PubMed:30373157}.
CC   -!- DOMAIN: The C-terminus regulates the targeting to host mitochondria.
CC       {ECO:0000269|PubMed:30373157}.
CC   -!- SIMILARITY: Belongs to the polerovirus movement protein P3a family.
CC       {ECO:0000305}.
CC   -!- CAUTION: This sequence initiates at a non-canonical ATA isoleucine
CC       codon. {ECO:0000305}.
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DR   EMBL; D00530; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR   Proteomes; UP000006723; Genome.
DR   GO; GO:0044177; C:host cell Golgi apparatus; IEA:UniProtKB-SubCell.
DR   GO; GO:0044193; C:host cell mitochondrial outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0044219; C:host cell plasmodesma; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Host cell junction; Host Golgi apparatus;
KW   Host membrane; Host mitochondrion; Host mitochondrion outer membrane;
KW   Membrane; Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..45
FT                   /note="Movement protein P3a"
FT                   /id="PRO_0000455351"
FT   TRANSMEM        9..29
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   45 AA;  4980 MW;  3DED059DB9AF3280 CRC64;
     MDYKFLAGFA LGFSSAIPFS VAGLYFVYLK ISSHVRSIVN EYGRG