P3F3B_DANRE
ID P3F3B_DANRE Reviewed; 443 AA.
AC P79745; B3DJH8; P31366; P79744; Q6PHH6;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1997, sequence version 1.
DT 03-AUG-2022, entry version 163.
DE RecName: Full=POU domain, class 3, transcription factor 3-B;
DE AltName: Full=Brain-specific homeobox/POU domain protein 1.0;
DE Short=Brain-1.0;
DE Short=zfBrn-1.0;
DE AltName: Full=POU domain protein 1;
DE Short=ZFPOU1;
DE AltName: Full=POU domain protein 23;
DE Short=ZP-23;
GN Name=pou3f3b; Synonyms=brn-1.0, pou1, pou23, zp23, zp23pou;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT), TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RC TISSUE=Neurula;
RX PubMed=1417821; DOI=10.1016/0006-291x(92)90464-v;
RA Matsuzaki T., Amanuma H., Takeda H.;
RT "A POU-domain gene of zebrafish, ZFPOU1, specifically expressed in the
RT developing neural tissues.";
RL Biochem. Biophys. Res. Commun. 187:1446-1453(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT), TISSUE SPECIFICITY,
RP AND DEVELOPMENTAL STAGE.
RC TISSUE=Neurula;
RX PubMed=9016656; DOI=10.1093/nar/24.24.4874;
RA Spaniol P., Bornmann C., Hauptmann G., Gerster T.;
RT "Class III POU genes of zebrafish are predominantly expressed in the
RT central nervous system.";
RL Nucleic Acids Res. 24:4874-4881(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS LONG AND SHORT).
RC TISSUE=Embryo, and Kidney;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 262-374 (ISOFORM SHORT), TISSUE SPECIFICITY,
RP AND DEVELOPMENTAL STAGE.
RC TISSUE=Pharyngula;
RX PubMed=8605028; DOI=10.1006/bbrc.1996.0274;
RA Sampath K., Stuart G.W.;
RT "Developmental expression of class III and IV POU domain genes in the
RT zebrafish.";
RL Biochem. Biophys. Res. Commun. 219:565-571(1996).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-317, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18307296; DOI=10.1021/pr700667w;
RA Lemeer S., Pinkse M.W.H., Mohammed S., van Breukelen B., den Hertog J.,
RA Slijper M., Heck A.J.R.;
RT "Online automated in vivo zebrafish phosphoproteomics: from large-scale
RT analysis down to a single embryo.";
RL J. Proteome Res. 7:1555-1564(2008).
CC -!- FUNCTION: Transcription factor that may play important roles in
CC patterning the embryonic brain.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=Long;
CC IsoId=P79745-1; Sequence=Displayed;
CC Name=Short;
CC IsoId=P79745-2; Sequence=VSP_002341;
CC -!- TISSUE SPECIFICITY: Predominantly expressed in the central nervous
CC system. {ECO:0000269|PubMed:1417821, ECO:0000269|PubMed:8605028,
CC ECO:0000269|PubMed:9016656}.
CC -!- DEVELOPMENTAL STAGE: First expressed at early neurula stage (9-12
CC hours). Expression then increases greatly at the 16-somite stage,
CC reached the maximum level at 36 hr of development, and thereafter
CC decreased. Only expressed in adults in the brain.
CC {ECO:0000269|PubMed:1417821, ECO:0000269|PubMed:8605028,
CC ECO:0000269|PubMed:9016656}.
CC -!- SIMILARITY: Belongs to the POU transcription factor family. Class-3
CC subfamily. {ECO:0000305}.
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DR EMBL; D13045; BAA02377.1; -; mRNA.
DR EMBL; Y07907; CAA69215.1; -; mRNA.
DR EMBL; Y07907; CAA69214.1; -; mRNA.
DR EMBL; BC056320; AAH56320.1; -; mRNA.
DR EMBL; BC056549; AAH56549.1; -; mRNA.
DR EMBL; BC163489; AAI63489.1; -; mRNA.
DR PIR; JH0710; JH0710.
DR RefSeq; NP_571177.2; NM_131102.3. [P79745-2]
DR RefSeq; NP_958855.2; NM_201447.2. [P79745-1]
DR AlphaFoldDB; P79745; -.
DR SMR; P79745; -.
DR STRING; 7955.ENSDARP00000124688; -.
DR iPTMnet; P79745; -.
DR PaxDb; P79745; -.
DR DNASU; 30321; -.
DR Ensembl; ENSDART00000149202; ENSDARP00000123893; ENSDARG00000095896. [P79745-2]
DR Ensembl; ENSDART00000149949; ENSDARP00000124688; ENSDARG00000095896. [P79745-1]
DR GeneID; 30321; -.
DR KEGG; dre:30321; -.
DR CTD; 30321; -.
DR ZFIN; ZDB-GENE-980526-140; pou3f3b.
DR eggNOG; KOG3802; Eukaryota.
DR GeneTree; ENSGT00940000164385; -.
DR HOGENOM; CLU_013065_1_2_1; -.
DR InParanoid; P79745; -.
DR OMA; CKRMFSE; -.
DR OrthoDB; 752252at2759; -.
DR PhylomeDB; P79745; -.
DR TreeFam; TF316413; -.
DR PRO; PR:P79745; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 6.
DR Bgee; ENSDARG00000095896; Expressed in brain and 35 other tissues.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0007420; P:brain development; IEA:InterPro.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00086; homeodomain; 1.
DR Gene3D; 1.10.260.40; -; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR InterPro; IPR013847; POU.
DR InterPro; IPR000327; POU_dom.
DR InterPro; IPR016362; TF_POU_3.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF00157; Pou; 1.
DR PIRSF; PIRSF002629; Transcription_factor_POU; 1.
DR PRINTS; PR00028; POUDOMAIN.
DR SMART; SM00389; HOX; 1.
DR SMART; SM00352; POU; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR SUPFAM; SSF47413; SSF47413; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
DR PROSITE; PS00035; POU_1; 1.
DR PROSITE; PS00465; POU_2; 1.
DR PROSITE; PS51179; POU_3; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Developmental protein; DNA-binding; Homeobox;
KW Nucleus; Phosphoprotein; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..443
FT /note="POU domain, class 3, transcription factor 3-B"
FT /id="PRO_0000100771"
FT DOMAIN 238..312
FT /note="POU-specific"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00530"
FT DNA_BIND 330..389
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 21..40
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 100..150
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 182..244
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 21..39
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 103..120
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 210..225
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 226..244
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 317
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18307296"
FT VAR_SEQ 407..443
FT /note="GHFLVDYLKDASLTGPSEPGDQRVTTTSSFHQVILAH -> VSADTPPPSMD
FT CKRMFSET (in isoform Short)"
FT /evidence="ECO:0000303|PubMed:1417821,
FT ECO:0000303|PubMed:8605028, ECO:0000303|PubMed:9016656,
FT ECO:0000303|Ref.3"
FT /id="VSP_002341"
FT CONFLICT 109
FT /note="M -> I (in Ref. 1; BAA02377)"
FT /evidence="ECO:0000305"
FT CONFLICT 116
FT /note="Q -> QQ (in Ref. 3; AAH56549/AAI63489)"
FT /evidence="ECO:0000305"
FT CONFLICT 135
FT /note="H -> N (in Ref. 1; BAA02377)"
FT /evidence="ECO:0000305"
FT CONFLICT 146
FT /note="H -> Y (in Ref. 1; BAA02377)"
FT /evidence="ECO:0000305"
FT CONFLICT 170
FT /note="S -> F (in Ref. 1; BAA02377)"
FT /evidence="ECO:0000305"
FT CONFLICT 174..175
FT /note="SQ -> FA (in Ref. 1; BAA02377)"
FT /evidence="ECO:0000305"
FT CONFLICT 185
FT /note="L -> H (in Ref. 1; BAA02377)"
FT /evidence="ECO:0000305"
FT CONFLICT 263
FT /note="T -> K (in Ref. 1; BAA02377)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 443 AA; 47453 MW; 5F2A6DC6BB333F5C CRC64;
MATAASNPYL ASSSILSSGS IVHSDSGGGM QQGSAAVTSV SGGYRGDPTV KMVQSDFMQG
AMAASNGGHM LSHAHQWVTS LPHAAAAAAA AAVAAAEAGS PWSSSPVGMT GSPQQQDVKN
NSGRDDLHSG TALHHRAPHL GPHQTHAGAW GSTTAAHIPS LTGSQQQQQS LIYSQPGGFT
VNGMLSPPGS QSLVHPGLVR GDTPELDHSS HHHHHHHQHQ HHQQAHHGVN SHDPHSDEDT
PTSDDLEHFA KQFKQRRIKL GFTQADVGLA LGTLYGNVFS QTTICRFEAL QLSFKNMCKL
KPLLNKWLEE ADSSTGSPTS IDKIAAQGRK RKKRTSIEVS VKGALESHFL KCPKPSAQEI
TSLADNLQLE KEVVRVWFCN RRQKEKRMTP PGVPQTPEDV YSQVGNGHFL VDYLKDASLT
GPSEPGDQRV TTTSSFHQVI LAH
