P3F4A_XENLA
ID P3F4A_XENLA Reviewed; 361 AA.
AC P31364; Q6DFJ0;
DT 01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT 15-DEC-2009, sequence version 2.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=POU domain, class 3, transcription factor 4-A;
DE AltName: Full=Transcription factor POU2;
DE Short=XlPOU 2;
DE Short=XlPOU2;
GN Name=pou3f4-a; Synonyms=pou2, pou3f4;
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL
RP STAGE, AND INDUCTION.
RC TISSUE=Brain;
RX PubMed=7720579; DOI=10.1242/dev.121.3.721;
RA Witta S.E., Agarwal V.R., Sato S.M.;
RT "XLPOU 2, a noggin-inducible gene, has direct neuralizing activity.";
RL Development 121:721-730(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Kidney;
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 127-361, TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RC TISSUE=Brain;
RX PubMed=1717323; DOI=10.1016/0012-1606(91)90294-d;
RA Agarwal V.R., Sato S.M.;
RT "XLPOU 1 and XLPOU 2, two novel POU domain genes expressed in the
RT dorsoanterior region of Xenopus embryos.";
RL Dev. Biol. 147:363-373(1991).
RN [4]
RP FUNCTION, AND INDUCTION.
RX PubMed=10559482; DOI=10.1016/s0925-4773(99)00204-x;
RA Matsuo-Takasaki M., Lim J.H., Sato S.M.;
RT "The POU domain gene, XlPOU 2 is an essential downstream determinant of
RT neural induction.";
RL Mech. Dev. 89:75-85(1999).
CC -!- FUNCTION: Transcriptional activator. Induces neural-specific gene
CC expression to act as a key regulator of neural differentiation.
CC {ECO:0000269|PubMed:10559482, ECO:0000269|PubMed:7720579}.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- TISSUE SPECIFICITY: From embryonic stage 10, expressed in the Spemann's
CC organizer. During gastrulation, expressed in both the involuting
CC mesoderm and the overlying neuroectoderm. During the neural plate and
CC neural fold stages, expressed in the entire neuroectoderm with
CC expression in discrete regions of the developing nervous system
CC persisting at later stages. Transiently expressed in the pronephros
CC from stages 24-32. In adults, expressed in the kidney and brain.
CC {ECO:0000269|PubMed:1717323, ECO:0000269|PubMed:7720579}.
CC -!- DEVELOPMENTAL STAGE: First expressed at embryonic stage 9, with
CC expression increasing at neurula stage and again at the tailbud stage.
CC Expression persists through development to adulthood.
CC {ECO:0000269|PubMed:1717323, ECO:0000269|PubMed:7720579}.
CC -!- INDUCTION: By nog, zic1 and zic3. {ECO:0000269|PubMed:10559482,
CC ECO:0000269|PubMed:7720579}.
CC -!- SIMILARITY: Belongs to the POU transcription factor family. Class-3
CC subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA41781.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; U17654; AAA92684.1; -; mRNA.
DR EMBL; BC076747; AAH76747.1; -; mRNA.
DR EMBL; X59055; CAA41781.1; ALT_INIT; mRNA.
DR RefSeq; NP_001094393.1; NM_001100923.1.
DR AlphaFoldDB; P31364; -.
DR SMR; P31364; -.
DR DNASU; 397929; -.
DR GeneID; 397929; -.
DR KEGG; xla:397929; -.
DR CTD; 397929; -.
DR Xenbase; XB-GENE-865294; pou3f4.L.
DR OMA; HNNHGSH; -.
DR OrthoDB; 752252at2759; -.
DR Proteomes; UP000186698; Chromosome 8L.
DR Bgee; 397929; Expressed in internal ear and 5 other tissues.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR GO; GO:0007420; P:brain development; IEA:InterPro.
DR CDD; cd00086; homeodomain; 1.
DR Gene3D; 1.10.260.40; -; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR InterPro; IPR013847; POU.
DR InterPro; IPR000327; POU_dom.
DR InterPro; IPR016362; TF_POU_3.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF00157; Pou; 1.
DR PIRSF; PIRSF002629; Transcription_factor_POU; 1.
DR PRINTS; PR00028; POUDOMAIN.
DR SMART; SM00389; HOX; 1.
DR SMART; SM00352; POU; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR SUPFAM; SSF47413; SSF47413; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
DR PROSITE; PS00035; POU_1; 1.
DR PROSITE; PS00465; POU_2; 1.
DR PROSITE; PS51179; POU_3; 1.
PE 2: Evidence at transcript level;
KW Activator; Developmental protein; DNA-binding; Homeobox; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..361
FT /note="POU domain, class 3, transcription factor 4-A"
FT /id="PRO_0000100765"
FT DOMAIN 186..260
FT /note="POU-specific"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00530"
FT DNA_BIND 278..337
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 100..131
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 150..189
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 333..361
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 105..131
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 167..189
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 347..361
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 127
FT /note="S -> G (in Ref. 3; CAA41781)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 361 AA; 39838 MW; 6F6BE1D6AC844F81 CRC64;
MATAASNPYS ILSSSSLVHA DSAVMQQGSP FRNPQKLLQS DYLQGVPCNG HPLGHHWVTS
LSDANPWSSS LASSPLDQQD IKPGREDLQL GAIIHHRSPH VNHHSPHTNH PNAWGASPAH
NSSLTSSGQP INIYSQPSFT VSGMLDHGEL TPPLPAGTTQ SLHPVLREPN DHVDLGSHHC
QDHSDEETPT SDELEQFAKQ FKQRRIKLGF TQADVGLALG TLYGNVFSQT TICRFEALQL
SFKNMCKLKP LLNKWLEEAD SSTGNPTSID KIAAQGRKRK KRTSIEVSVK GVLETHFLKC
PKPAALEITS LADSLQLEKE VVRVWFCNRR QKEKRMTPPG DPQQHEVYSH SVKTDTSCNE
L
