P3IP1_MOUSE
ID P3IP1_MOUSE Reviewed; 264 AA.
AC Q7TMJ8; Q3TTD5; Q5NBY3; Q5NBY4; Q5NBY6; Q811Z2; Q811Z3;
DT 20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Phosphoinositide-3-kinase-interacting protein 1;
DE AltName: Full=Calcineurin-regulated kringle domain-containing protein;
DE AltName: Full=Kringle domain-containing protein HGFL;
DE Flags: Precursor;
GN Name=Pik3ip1; Synonyms=Crkd, Hgfl;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=BALB/cJ;
RA Chiang H., Chang M.;
RL Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC STRAIN=CD-1;
RA Corbit K.C., Kuo A.C., Chen F., Crabtree G.R.;
RT "Calcineurin-NFAT signaling represses CRKD, an embryonic mammary marker
RT secreted in breast cancer.";
RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J, and NOD; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J, and FVB/N; TISSUE=Brain, and Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP FUNCTION.
RX PubMed=18632611; DOI=10.1158/0008-5472.can-08-0025;
RA He X., Zhu Z., Johnson C., Stoops J., Eaker A.E., Bowen W., DeFrances M.C.;
RT "PIK3IP1, a negative regulator of PI3K, suppresses the development of
RT hepatocellular carcinoma.";
RL Cancer Res. 68:5591-5598(2008).
CC -!- FUNCTION: Negative regulator of hepatic phosphatidylinositol 3-kinase
CC (PI3K) activity. {ECO:0000269|PubMed:18632611}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q96FE7};
CC Single-pass type I membrane protein {ECO:0000255}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=HGFL(L);
CC IsoId=Q7TMJ8-1; Sequence=Displayed;
CC Name=2; Synonyms=HGFL(S);
CC IsoId=Q7TMJ8-2; Sequence=VSP_023641, VSP_023642;
CC -!- SEQUENCE CAUTION:
CC Sequence=CAI35211.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAI35213.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAI35214.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AF528078; AAO33761.1; -; mRNA.
DR EMBL; AF528081; AAO33764.1; -; mRNA.
DR EMBL; AY522649; AAS13454.1; -; mRNA.
DR EMBL; AK154842; BAE32868.1; -; mRNA.
DR EMBL; AK161428; BAE36390.1; -; mRNA.
DR EMBL; AL671968; CAI35210.1; -; Genomic_DNA.
DR EMBL; AL671968; CAI35211.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL671968; CAI35212.1; -; Genomic_DNA.
DR EMBL; AL671968; CAI35213.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL671968; CAI35214.1; ALT_SEQ; Genomic_DNA.
DR EMBL; BC055920; AAH55920.1; -; mRNA.
DR EMBL; BC079641; AAH79641.1; -; mRNA.
DR CCDS; CCDS24357.1; -. [Q7TMJ8-1]
DR RefSeq; NP_835362.2; NM_178149.4. [Q7TMJ8-1]
DR AlphaFoldDB; Q7TMJ8; -.
DR STRING; 10090.ENSMUSP00000036921; -.
DR iPTMnet; Q7TMJ8; -.
DR PhosphoSitePlus; Q7TMJ8; -.
DR PaxDb; Q7TMJ8; -.
DR PeptideAtlas; Q7TMJ8; -.
DR PRIDE; Q7TMJ8; -.
DR ProteomicsDB; 287750; -. [Q7TMJ8-1]
DR ProteomicsDB; 287751; -. [Q7TMJ8-2]
DR ABCD; Q7TMJ8; 5 sequenced antibodies.
DR Antibodypedia; 1229; 136 antibodies from 22 providers.
DR Ensembl; ENSMUST00000045153; ENSMUSP00000036921; ENSMUSG00000034614. [Q7TMJ8-1]
DR Ensembl; ENSMUST00000093399; ENSMUSP00000091100; ENSMUSG00000034614. [Q7TMJ8-2]
DR GeneID; 216505; -.
DR KEGG; mmu:216505; -.
DR UCSC; uc007hsp.1; mouse. [Q7TMJ8-2]
DR UCSC; uc007hsq.1; mouse. [Q7TMJ8-1]
DR CTD; 113791; -.
DR MGI; MGI:1917016; Pik3ip1.
DR VEuPathDB; HostDB:ENSMUSG00000034614; -.
DR eggNOG; ENOG502QTWD; Eukaryota.
DR GeneTree; ENSGT00390000017774; -.
DR HOGENOM; CLU_092099_0_0_1; -.
DR InParanoid; Q7TMJ8; -.
DR OMA; DQKVCER; -.
DR OrthoDB; 1027586at2759; -.
DR PhylomeDB; Q7TMJ8; -.
DR TreeFam; TF331319; -.
DR BioGRID-ORCS; 216505; 2 hits in 71 CRISPR screens.
DR ChiTaRS; Pik3ip1; mouse.
DR PRO; PR:Q7TMJ8; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q7TMJ8; protein.
DR Bgee; ENSMUSG00000034614; Expressed in interventricular septum and 224 other tissues.
DR ExpressionAtlas; Q7TMJ8; baseline and differential.
DR Genevisible; Q7TMJ8; MM.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0036313; F:phosphatidylinositol 3-kinase catalytic subunit binding; ISO:MGI.
DR GO; GO:0043553; P:negative regulation of phosphatidylinositol 3-kinase activity; IDA:UniProtKB.
DR GO; GO:0014067; P:negative regulation of phosphatidylinositol 3-kinase signaling; IDA:UniProtKB.
DR CDD; cd00108; KR; 1.
DR Gene3D; 2.40.20.10; -; 1.
DR InterPro; IPR000001; Kringle.
DR InterPro; IPR013806; Kringle-like.
DR InterPro; IPR018056; Kringle_CS.
DR InterPro; IPR038178; Kringle_sf.
DR Pfam; PF00051; Kringle; 1.
DR SMART; SM00130; KR; 1.
DR SUPFAM; SSF57440; SSF57440; 1.
DR PROSITE; PS00021; KRINGLE_1; 1.
DR PROSITE; PS50070; KRINGLE_2; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell membrane; Disulfide bond; Kringle; Membrane;
KW Reference proteome; Signal; Transmembrane; Transmembrane helix.
FT SIGNAL 1..21
FT /evidence="ECO:0000250"
FT CHAIN 22..264
FT /note="Phosphoinositide-3-kinase-interacting protein 1"
FT /id="PRO_0000280348"
FT TOPO_DOM 22..170
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 171..191
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 192..264
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 24..101
FT /note="Kringle"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00121"
FT REGION 94..129
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT DISULFID 25..101
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00121"
FT DISULFID 46..82
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00121"
FT DISULFID 70..96
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00121"
FT VAR_SEQ 198..213
FT /note="GKDLKEQHEKKACERE -> WVLSPPGSLGIGLRKT (in isoform 2)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_023641"
FT VAR_SEQ 214..264
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_023642"
FT CONFLICT 243
FT /note="Q -> R (in Ref. 1; AAO33764)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 264 AA; 28567 MW; 833EA578FEB763A4 CRC64;
MLLAWVHTFL LSNMLLAEAY GSGGCFWDNG HLYREDQPSP APGLRCLNWL AAQGSRESLT
EPSPGNHNYC RNPDQDPRGP WCYISSETGV PEKRPCEDVS CPETTSQAPP PSSAMELEEK
SGAPGDKEAQ VFPPANALPA RSEAAEVQPV IGISQLVRMN SKEKKDLGTL GYVLGITMMV
IILAIGAGII VGYTYKRGKD LKEQHEKKAC EREMQRITLP LSAFTNPTCE TVDENTIIVH
SNQTPADVQE GSTLLTGQAG TPGA
