ID   P47K_PSECL              Reviewed;         419 AA.
AC   P31521;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1993, sequence version 1.
DT   03-AUG-2022, entry version 59.
DE   RecName: Full=47 kDa protein;
DE   AltName: Full=P47K;
OS   Pseudomonas chlororaphis (Pseudomonas aureofaciens).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=B23;
RX   PubMed=2013568; DOI=10.1128/jb.173.8.2465-2472.1991;
RA   Nishiyama M., Horinouchi S., Kobayashi M., Nagasawa T., Yamada H.,
RA   Beppu T.;
RT   "Cloning and characterization of genes responsible for metabolism of
RT   nitrile compounds from Pseudomonas chlororaphis B23.";
RL   J. Bacteriol. 173:2465-2472(1991).
CC   -!- FUNCTION: The presence of P47K is critical for the expression of the
CC       nitrile hydratase genes. May stabilize or activate the nitrile
CC       hydratase proteins.
CC   -!- SIMILARITY: Belongs to the SIMIBI class G3E GTPase family. CobW
CC       subfamily. {ECO:0000305}.
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DR   EMBL; D90216; BAA14247.1; -; Genomic_DNA.
DR   PIR; D42725; D42725.
DR   AlphaFoldDB; P31521; -.
DR   SMR; P31521; -.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.1220.10; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR011629; Cbl_biosynth_CobW-like_C.
DR   InterPro; IPR036627; CobW-likC_sf.
DR   InterPro; IPR003495; CobW/HypB/UreG_dom.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF02492; cobW; 1.
DR   Pfam; PF07683; CobW_C; 1.
DR   SMART; SM00833; CobW_C; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Nucleotide-binding.
FT   CHAIN           1..419
FT                   /note="47 kDa protein"
FT                   /id="PRO_0000058140"
FT   DOMAIN          262..379
FT                   /note="CobW C-terminal"
FT   BINDING         17..24
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
FT   BINDING         79..83
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
FT   BINDING         187..190
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   419 AA;  46666 MW;  FF5113800E27FF0C CRC64;
     MIEGAQAGRL PVTVLSGFLG AGKTTLLNAI LRNRQGLRVA VIVNDMSEVN LDAESVQRDV
     SLHRGRDELI EMSNGCICCT LRADLLEQIS DLARQQRFDY LLIESTGISE PMPVAETFAF
     LDTEGFSLSE LARLDTLVTV VDGSQFQALL ESTDTVARAD TEAHTSTRHL ADLLIEQVEY
     ANVILVNKRD LIDEPGYQAV HAILAGLNPS ARIMPMAHGN VALSSLLDTH LFDLPSLAAS
     PGWMRKMEAT DTPASESDTY GVTSWVYRER APFHPQRLLE FLQKPWHNGR LLRSKGYFWL
     ASRHLEIGLL AQSGKQFQWD YVGRWWNFIE PSQWPRDEYR LQGIMAKWDS VVGDCRQELV
     FIGQGLDTRV LQRELDHCLL SAQEIAAGPL AWQALPAATA FDTEALSARP TPPMAVQPT