ID   P49_ASFB7               Reviewed;         438 AA.
AC   Q65165;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   23-FEB-2022, entry version 64.
DE   RecName: Full=Minor capsid protein p49 {ECO:0000303|PubMed:31624094};
DE            Short=p49;
GN   OrderedLocusNames=Ba71V-075; ORFNames=B438L;
OS   African swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V)
OS   (ASFV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Asfuvirales; Asfarviridae; Asfivirus.
OX   NCBI_TaxID=10498;
OH   NCBI_TaxID=6937; Ornithodoros (relapsing fever ticks).
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=11831707; DOI=10.1006/viro.1995.1149;
RA   Yanez R.J., Rodriguez J.M., Nogal M.L., Yuste L., Enriquez C.,
RA   Rodriguez J.F., Vinuela E.;
RT   "Analysis of the complete nucleotide sequence of African swine fever
RT   virus.";
RL   Virology 208:249-278(1995).
RN   [2]
RP   CHARACTERIZATION, SUBCELLULAR LOCATION, AND INDUCTION.
RX   PubMed=10640542; DOI=10.1099/0022-1317-81-1-59;
RA   Galindo I., Vinuela E., Carrascosa A.L.;
RT   "Characterization of the african swine fever virus protein p49: a new late
RT   structural polypeptide.";
RL   J. Gen. Virol. 81:59-65(2000).
RN   [3]
RP   FUNCTION.
RX   PubMed=17005638; DOI=10.1128/jvi.01468-06;
RA   Epifano C., Krijnse-Locker J., Salas M.L., Salas J., Rodriguez J.M.;
RT   "Generation of filamentous instead of icosahedral particles by repression
RT   of African swine fever virus structural protein pB438L.";
RL   J. Virol. 80:11456-11466(2006).
RN   [4]
RP   SUBCELLULAR LOCATION.
RX   PubMed=30185597; DOI=10.1128/jvi.01293-18;
RA   Alejo A., Matamoros T., Guerra M., Andres G.;
RT   "A Proteomic Atlas of the African Swine Fever Virus Particle.";
RL   J. Virol. 92:0-0(2018).
RN   [5]
RP   INDUCTION.
RX   PubMed=32075923; DOI=10.1128/jvi.00119-20;
RA   Cackett G., Matelska D., Sykora M., Portugal R., Malecki M., Baehler J.,
RA   Dixon L., Werner F.;
RT   "The African Swine Fever Virus Transcriptome.";
RL   J. Virol. 94:0-0(2020).
RN   [6]
RP   STRUCTURE BY ELECTRON MICROSCOPY (4.8 ANGSTROMS) OF THE ASFV CAPSID,
RP   FUNCTION, SUBCELLULAR LOCATION, AND IDENTIFICATION.
RX   PubMed=31624094; DOI=10.1126/science.aaz1439;
RA   Wang N., Zhao D., Wang J., Zhang Y., Wang M., Gao Y., Li F., Wang J.,
RA   Bu Z., Rao Z., Wang X.;
RT   "Architecture of African swine fever virus and implications for viral
RT   assembly.";
RL   Science 366:640-644(2019).
CC   -!- FUNCTION: Together with the penton and the other minor capsid proteins
CC       (M1249L, p17), forms a complicated network immediately below the outer
CC       capsid shell, stabilizing the whole capsid (PubMed:31624094). Plays an
CC       essential role in the formation of infectious virus particles.
CC       Especially required for the formation of the capsid vertices
CC       (PubMed:17005638). During virion assembly, associates with the membrane
CC       and probably mediates the docking of the penton complex to the inner
CC       membrane, where it recruits the capsomers to form the penton core
CC       (PubMed:31624094). {ECO:0000269|PubMed:17005638,
CC       ECO:0000269|PubMed:31624094}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000269|PubMed:10640542,
CC       ECO:0000269|PubMed:30185597}. Note=Localizes in close proximity to the
CC       capsid vertices. {ECO:0000269|PubMed:30185597}.
CC   -!- INDUCTION: Expressed in the late phase of the viral replicative cycle.
CC       {ECO:0000269|PubMed:10640542, ECO:0000269|PubMed:32075923}.
CC   -!- SIMILARITY: Belongs to the asfivirus p49 structural protein family.
CC       {ECO:0000305}.
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DR   EMBL; U18466; AAA65305.1; -; Genomic_DNA.
DR   RefSeq; NP_042769.1; NC_001659.2.
DR   GeneID; 22220305; -.
DR   KEGG; vg:22220305; -.
DR   Proteomes; UP000000624; Genome.
PE   1: Evidence at protein level;
KW   Late protein; Reference proteome; Virion.
FT   CHAIN           1..438
FT                   /note="Minor capsid protein p49"
FT                   /id="PRO_0000373410"
FT   REGION          145..167
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   438 AA;  49312 MW;  7062CD443676D3C4 CRC64;
     MYHDYASKLL ADYRSDPPLW ESDLPRHNRY SDNILNSRYC GNKNGAAPVY NEYTNSPEKA
     EKGLQLSDLR NFSFMLNPQH KNIGYGDAQD LEPYSSIPKN KLFNHFKNHR PAFSTHTENL
     IRRNVVRTEK KTFPQVASLK GTQKNCLTQP SSLPSLKNPK NSSVPSTRFS EHTKFFSYED
     IPKLKTKGTI KHEQHLGDQM PGQHYNGYIP HKDVYNILCL AHNLPASVEK GIAGRGIPLG
     NPHVKPNIEQ ELIKSTSTYT GVPMLGPLPP KDSQHGREYQ EFSANRHMLQ VANILHSVFA
     NHSIKPQILE DIPVLNAQLT SIKPVSPFLN KAYQTHYMEN IVTLVPRFKS IANYSSPIPN
     YSKRNSGQAE YFDTSKQTIS RHNNYIPKYT GGIGDSKLDS TFPKDFNASS VPLTSAEKDH
     SLRGDNSACC ISSISPSL