ID   P4A_VACCC               Reviewed;         891 AA.
AC   P20642;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1991, sequence version 1.
DT   25-MAY-2022, entry version 66.
DE   RecName: Full=Major core protein 4a precursor;
DE            Short=p4a;
DE   AltName: Full=Virion core protein 4a precursor;
DE   Contains:
DE     RecName: Full=Core protein 4a;
DE     AltName: Full=62 kDa peptide;
DE   Contains:
DE     RecName: Full=23 kDa protein;
DE              Short=23K;
DE   Flags: Precursor;
GN   ORFNames=A10L;
OS   Vaccinia virus (strain Copenhagen) (VACV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=10249;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=2219722; DOI=10.1016/0042-6822(90)90294-2;
RA   Goebel S.J., Johnson G.P., Perkus M.E., Davis S.W., Winslow J.P.,
RA   Paoletti E.;
RT   "The complete DNA sequence of vaccinia virus.";
RL   Virology 179:247-266(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Goebel S.J., Johnson G.P., Perkus M.E., Davis S.W., Winslow J.P.,
RA   Paoletti E.;
RT   "Appendix to 'The complete DNA sequence of vaccinia virus'.";
RL   Virology 179:517-563(1990).
CC   -!- FUNCTION: Core protein 4a is the most abundant virion protein. Major
CC       component of the virion core that undergoes proteolytic processing
CC       during the immature virion (IV) to mature virion (MV) transition (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with P39/A4. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: [Core protein 4a]: Virion {ECO:0000305}.
CC       Note=Probably localizes to the virion core wall. {ECO:0000250}.
CC   -!- PTM: The precursor is cleaved by I7 to give rise to the 62 kDa mature
CC       protein during virion maturation. Proteolytic cleavage of major core
CC       proteins P4a (A10L), P4b (A3L), and VP8 (L4R), which occurs at a late
CC       stage of core formation, is required for production of infectious
CC       mature virions (MV) (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the poxviridae protein P4a family.
CC       {ECO:0000305}.
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DR   EMBL; M35027; AAA48129.1; -; Genomic_DNA.
DR   PIR; C42518; WMVZ3S.
DR   SMR; P20642; -.
DR   Proteomes; UP000008269; Genome.
DR   GO; GO:0044423; C:virion component; IEA:InterPro.
DR   GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR   InterPro; IPR005058; Poxvirus_P4A.
DR   Pfam; PF03395; Pox_P4A; 1.
PE   3: Inferred from homology;
KW   Reference proteome; Virion.
FT   CHAIN           1..891
FT                   /note="Major core protein 4a precursor"
FT                   /id="PRO_0000413907"
FT   CHAIN           1..614
FT                   /note="Core protein 4a"
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000040572"
FT   PROPEP          615..697
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000413908"
FT   CHAIN           698..891
FT                   /note="23 kDa protein"
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000040574"
SQ   SEQUENCE   891 AA;  102284 MW;  6360857BC36C61EA CRC64;
     MMPIKSIVTL DQLEDSEYLF RIVSTVLPHL CLDYKVCDKL KTTFVHPFDI LLNNSLGSVT
     KQDELQAAIS KLGINYLIDT TSRELKLFNV TLNAGNIDII NTPINISSET NPIINTHSFY
     DLPPFTQHLL NIRLTDTEYR ARFIGGYIKP DGSDSMDVLA EKKYPDLNFD NTYLFNILYK
     DVINAPIKEF KAKIVNGVLS RQDFDNLIGV RQYITAQDRP RFDNTYNIAD AARHYGVNLN
     TLPLPNVDLT TMPTYKHLIM FEQYFIYTYD RVDIYYNGNK MLFDDEIMNF CISMRYQSLI
     PRLVDFFPDI PVNNNIVLHT RDPQNAAVNV TVALPNVQFV DINRNNKFFI NFFNLLAKEQ
     RSTAIKVTKS MFWDGMDYEE YKSKNLQDMM FINSTCYVFG LYNHNNTTYC SILSDIISAE
     KTPIRVCLLP RVVGGKTVTN LISETLKSIS SMTIREFPRK DKSIMHIGLS ETGFMRFFQL
     LRLMADKPHE TAIKEVVMAY VGIKLGDKGS PYYIRKESYQ DFIYLLFASM GFKVTTRRSI
     MGSNNISIIS IRPRVTKQYI VTTLMKTSCS KNEAEKLITS AFDLLNFMVS VSDFRDYQSY
     RQYRNYCPRY FYAGSPEGEE TIICDSEPIS ILDRIDTRGI FSAYTINEMM DTDIFSPENK
     AFKNNLSRFI ESGDITGEDI FCAMPYNILD RIITNAGTCT VSIGDMLDNI TTQSDCNMTN
     EITDMINASL KNTISKDNNM LVSQALNSVA NRSKQTIGDL RQSSCKMALL FKNLATSIYT
     IERIFNAKVG DDVKASMLEK YKVFTDISMS LYKDLIAMEN LKAMLYIIRR SGCRIDDAQI
     TTDDLVKSYS LIRPKILSMI NYYNEMSRGY FEHMKKNLNM TDGDSVSFDD E