P4A_VACCW
ID P4A_VACCW Reviewed; 891 AA.
AC P16715; Q76ZQ5;
DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1990, sequence version 1.
DT 25-MAY-2022, entry version 79.
DE RecName: Full=Major core protein 4a precursor;
DE Short=p4a;
DE AltName: Full=Virion core protein 4a precursor;
DE Contains:
DE RecName: Full=Core protein 4a;
DE AltName: Full=62 kDa peptide;
DE Contains:
DE RecName: Full=23 kDa protein;
DE Short=23K;
DE Flags: Precursor;
GN OrderedLocusNames=VACWR129; ORFNames=A10L;
OS Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain
OS WR)).
OC Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX NCBI_TaxID=10254;
OH NCBI_TaxID=9913; Bos taurus (Bovine).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3196167; DOI=10.1007/bf01315559;
RA van Meir E., Wittek R.;
RT "Fine structure of the vaccinia virus gene encoding the precursor of the
RT major core protein 4 a.";
RL Arch. Virol. 102:19-27(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J.,
RA Wohlhueter R.;
RT "Sequencing of the coding region of Vaccinia-WR to an average 9-fold
RT redundancy and an error rate of 0.16/10kb.";
RL Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP PROTEIN SEQUENCE OF 698-706, AND PROTEOLYTIC PROCESSING.
RX PubMed=1853556; DOI=10.1016/0042-6822(91)90976-i;
RA van Slyke J.K., Whitehead S.S., Wilson E.M., Hruby D.E.;
RT "The multistep proteolytic maturation pathway utilized by vaccinia virus
RT P4a protein: a degenerate conserved cleavage motif within core proteins.";
RL Virology 183:467-478(1991).
RN [4]
RP PROTEOLYTIC PROCESSING.
RX PubMed=7897360; DOI=10.1099/0022-1317-76-3-717;
RA Whitehead S.S., Bersani N.A., Hruby D.E.;
RT "Physical and molecular genetic analysis of the multistep proteolytic
RT maturation pathway utilized by vaccinia virus P4a protein.";
RL J. Gen. Virol. 76:717-721(1995).
RN [5]
RP INTERACTION WITH P39/A4.
RX PubMed=10600608; DOI=10.1006/viro.1999.0046;
RA Risco C., Rodriguez J.R., Demkowicz W., Heljasvaara R., Carrascosa J.L.,
RA Esteban M., Rodriguez D.;
RT "The vaccinia virus 39-kDa protein forms a stable complex with the p4a/4a
RT major core protein early in morphogenesis.";
RL Virology 265:375-386(1999).
RN [6]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=11390580; DOI=10.1128/jvi.75.13.5778-5795.2001;
RA Heljasvaara R., Rodriguez D., Risco C., Carrascosa J.L., Esteban M.,
RA Rodriguez J.R.;
RT "The major core protein P4a (A10L gene) of vaccinia virus is essential for
RT correct assembly of viral DNA into the nucleoprotein complex to form
RT immature viral particles.";
RL J. Virol. 75:5778-5795(2001).
CC -!- FUNCTION: Core protein 4a is the most abundant virion protein. Major
CC component of the virion core that undergoes proteolytic processing
CC during the immature virion (IV) to mature virion (MV) transition.
CC {ECO:0000269|PubMed:11390580}.
CC -!- SUBUNIT: Interacts with P39/A4. {ECO:0000269|PubMed:10600608}.
CC -!- SUBCELLULAR LOCATION: [Core protein 4a]: Virion {ECO:0000305}.
CC Note=Probably localizes to the virion core wall.
CC -!- PTM: The precursor is cleaved by I7 to give rise to the 62 kDa mature
CC protein during virion maturation. Proteolytic cleavage of major core
CC proteins P4a (A10L), P4b (A3L), and VP8 (L4R), which occurs at a late
CC stage of core formation, is required for production of infectious
CC mature virions (MV). {ECO:0000269|PubMed:1853556,
CC ECO:0000269|PubMed:7897360}.
CC -!- SIMILARITY: Belongs to the poxviridae protein P4a family.
CC {ECO:0000305}.
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DR EMBL; M27634; AAA69585.1; -; Genomic_DNA.
DR EMBL; AY243312; AAO89408.1; -; Genomic_DNA.
DR PIR; A43497; WMVZ3W.
DR RefSeq; YP_233011.1; NC_006998.1.
DR DIP; DIP-2171N; -.
DR MINT; P16715; -.
DR PRIDE; P16715; -.
DR DNASU; 3707527; -.
DR GeneID; 3707527; -.
DR KEGG; vg:3707527; -.
DR Proteomes; UP000000344; Genome.
DR GO; GO:0044423; C:virion component; IEA:InterPro.
DR GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR InterPro; IPR005058; Poxvirus_P4A.
DR Pfam; PF03395; Pox_P4A; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Reference proteome; Virion.
FT CHAIN 1..891
FT /note="Major core protein 4a precursor"
FT /id="PRO_0000413909"
FT CHAIN 1..614
FT /note="Core protein 4a"
FT /id="PRO_0000040575"
FT PROPEP 615..697
FT /evidence="ECO:0000269|PubMed:1853556"
FT /id="PRO_0000413910"
FT CHAIN 698..891
FT /note="23 kDa protein"
FT /id="PRO_0000040577"
FT SITE 614..615
FT /note="Cleavage; by I7"
FT SITE 697..698
FT /note="Cleavage; by I7"
SQ SEQUENCE 891 AA; 102274 MW; 462DD9937643FBB3 CRC64;
MMPIKSIVTL DQLEDSEYLF RIVSTVLPHL CLDYKVCDQL KTTFVHPFDI LLNNSLGSVT
KQDELQAAIS KLGINYLIDT TSRELKLFNV TLNAGNIDII NTPINISSET NPIINTHSFY
DLPPFTQHLL NIRLTDTEYR ARFIGGYIKP DGSDSMDVLA EKKYPDLNFD NTYLFNILYK
DVINAPIKEF KAKIVNGVLS RQDFDNLIGV RQYITIQDRP RFDDAYNIAD AARHYGVNLN
TLPLPNVDLT TMPTYKHLIM FEQYFIYTYD RVDIYYNGNK MLFDDEIINF TISMRYQSLI
PRLVDFFPDI PVNNNIVLHT RDPQNAAVNV TVALPNVQFV DINRNNKFFI NFFNLLAKEQ
RSTAIKVTKS MFWDGMDYEE YKSKNLQDMM FINSTCYVFG LYNHNNTTYC SILSDIISAE
KTPIRVCLLP RVVGGKTVTN LISETLKSIS SMTIREFPRK DKSIMHIGLS ETGFMRFFQL
LRLMADKPHE TAIKEVVMAY VGIKLGDKGS PYYIRKESYQ DFIYLLFASM GFKVTTRRSI
MGSNNISIIS IRPRVTKQYI VATLMKTSCS KNEAEKLITS AFDLLNFMVS VSDFRDYQSY
RQYRNYCPRY FYAGSPEGEE TIICDSEPIS ILDRIDTRGI FSAYTINEMM DTDIFSPENK
AFKNNLSRFI ESGDITGEDI FCAMPYNILD RIITNAGTCT VSIGDMLDNI TTQSDCNMTN
EITDMINASL KNTISKDNNM LVSQALNSVA NRSKQKIGDL RQSSCKMALL FKNLATSIYT
IERIFNAKVG DDVKASMLEK YKVFTDISMS LYKDLIAMEN LKAMLYIIRR SGCRIDDAQI
TTDDLVKSYS LIRPKILSMI NYYNEMSRGY FEHMKKNLNM TDGDSVSFDD E
