ID   P4A_VARV                Reviewed;         892 AA.
AC   P0DOL2; P33817;
DT   16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT   16-OCT-2019, sequence version 1.
DT   25-MAY-2022, entry version 12.
DE   RecName: Full=Major core protein 4a precursor;
DE            Short=p4a;
DE   AltName: Full=Virion core protein 4a precursor;
DE   Contains:
DE     RecName: Full=Core protein 4a;
DE     AltName: Full=62 kDa peptide;
DE   Contains:
DE     RecName: Full=23 kDa protein;
DE              Short=23K;
DE   Flags: Precursor;
GN   ORFNames=A10L;
OS   Variola virus.
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus.
OX   NCBI_TaxID=10255;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Bangladesh-1975;
RX   PubMed=8264798; DOI=10.1038/366748a0;
RA   Massung R.F., Esposito J.J., Liu L.I., Qi J., Utterback T.R., Knight J.C.,
RA   Aubin L., Yuran T.E., Parsons J.M., Loparev V.N., Selivanov N.A.,
RA   Cavallaro K.F., Kerlavage A.R., Mahy B.W.J., Venter J.C.;
RT   "Potential virulence determinants in terminal regions of variola smallpox
RT   virus genome.";
RL   Nature 366:748-751(1993).
CC   -!- FUNCTION: Core protein 4a is the most abundant virion protein. Major
CC       component of the virion core that undergoes proteolytic processing
CC       during the immature virion (IV) to mature virion (MV) transition (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with P39/A4.
CC   -!- SUBCELLULAR LOCATION: [Core protein 4a]: Virion {ECO:0000250}.
CC       Note=Probably localizes to the virion core wall. {ECO:0000250}.
CC   -!- PTM: The precursor is cleaved by I7 to give rise to the 62 kDa mature
CC       protein during virion maturation. Proteolytic cleavage of major core
CC       proteins P4a (A10L), P4b (A3L), and VP8 (L4R), which occurs at a late
CC       stage of core formation, is required for production of infectious
CC       mature virions (MV) (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the poxviridae protein P4a family.
CC       {ECO:0000305}.
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DR   EMBL; L22579; AAA60862.1; -; Genomic_DNA.
DR   PIR; T28552; T28552.
DR   RefSeq; NP_042158.1; NC_001611.1.
DR   GeneID; 1486485; -.
DR   KEGG; vg:1486485; -.
DR   Proteomes; UP000119805; Genome.
DR   GO; GO:0044423; C:virion component; IEA:InterPro.
DR   GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR   InterPro; IPR005058; Poxvirus_P4A.
DR   Pfam; PF03395; Pox_P4A; 1.
PE   3: Inferred from homology;
KW   Virion.
FT   CHAIN           1..892
FT                   /note="Major core protein 4a precursor"
FT                   /id="PRO_0000448141"
FT   CHAIN           1..615
FT                   /note="Core protein 4a"
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000448142"
FT   PROPEP          616..698
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000448143"
FT   CHAIN           699..892
FT                   /note="23 kDa protein"
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000448144"
SQ   SEQUENCE   892 AA;  102262 MW;  AE946EE286B7D33B CRC64;
     MMPIKSIVTL DQLEDSEYLF RIVSTVLPHL CLDYKVCDQL KTTFVHPFDV FLNNSLGSVT
     KQDELQAAIS KLGINYLIDT TSRELKLFNV TLNAGNIDII NTPINISSET NPIINTHSFY
     DLPPFTQHLL NIRLTDTEYR ARFIGGYIKP DGSDSMDVLA EKKYPDLNFD NTYLFNILYK
     DVINAPIKEF KAKIVNGVLS RQDFDNLIGV RQYITAQDQP RFDNTYAIAD AARHYGVNLN
     TLPLPNVDLT TMPTYKHLIM YEQYFVDDYD RVPIYYNGNR VIFDDEIINF CISMRYQSLI
     PRLVEFFPDI PVNNNIVLHT RDPQNAAVNV TVGLPNMQFV DINRNNKFFI NFFNLLAKEQ
     RSTAIKVTKS MFWDGMDYEE YKSKNLQDMM FINSTCYVFG LYNHNNTTYC SILSDIISAE
     KTPIRVCLLP RVVGGKTVTD LISETLKSIS SMTIREFPKK DKSSIMHIGL SETGFMRFFQ
     LLRLMADKPH ETAIKEVVMA YVGIKLGDKG SPYYIRKESY QDFIYLLFAS MGFKVTTRRS
     IMGSNNISII SIRPRVTKQY IVTTLMKTSC SKNEAEKLIT SAFDLLNFMV SVSDFRDYQS
     YRQYRNYCPR YFYAGSPEGE ETIICDSEPI SILDRIDTRG IFSAYTINEM MDTDIFSPEN
     KAFKNNLSRF IESGDITGED IFCAMPYNIL DRIITNAGTC TVSIGDMLDN ITTQSDCNMT
     NEITDMINAS LKNTISKDNN MLVSQALDSV ANHSKQKIGD LRQSSCKMAL LFKNLATSIY
     TIERIFNAKV GDDVKASMLE KYKVFTDISM SLYKDLIAME NLKAMLYIIR RSGCRIDDAQ
     ITTDDLVKSY SLIRPKILSM INYYNEMSRG YFEHMKKNLN MTDGDSVSFD DE