ASH1_CANAL
ID ASH1_CANAL Reviewed; 449 AA.
AC Q5A5Q6; A0A1D8PIR3; Q8TGB9;
DT 26-JUN-2013, integrated into UniProtKB/Swiss-Prot.
DT 26-APR-2005, sequence version 1.
DT 03-AUG-2022, entry version 95.
DE RecName: Full=Transcriptional regulatory protein ASH1;
GN Name=ASH1; OrderedLocusNames=CAALFM_C210660WA;
GN ORFNames=CaO19.12803, CaO19.5343;
OS Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX NCBI_TaxID=237561;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=15123810; DOI=10.1073/pnas.0401648101;
RA Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B.,
RA Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W.,
RA Scherer S.;
RT "The diploid genome sequence of Candida albicans.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=17419877; DOI=10.1186/gb-2007-8-4-r52;
RA van het Hoog M., Rast T.J., Martchenko M., Grindle S., Dignard D.,
RA Hogues H., Cuomo C., Berriman M., Scherer S., Magee B.B., Whiteway M.,
RA Chibana H., Nantel A., Magee P.T.;
RT "Assembly of the Candida albicans genome into sixteen supercontigs aligned
RT on the eight chromosomes.";
RL Genome Biol. 8:RESEARCH52.1-RESEARCH52.12(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=24025428; DOI=10.1186/gb-2013-14-9-r97;
RA Muzzey D., Schwartz K., Weissman J.S., Sherlock G.;
RT "Assembly of a phased diploid Candida albicans genome facilitates allele-
RT specific measurements and provides a simple model for repeat and indel
RT structure.";
RL Genome Biol. 14:RESEARCH97.1-RESEARCH97.14(2013).
RN [4]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=12073088; DOI=10.1007/s00294-002-0286-y;
RA Munchow S., Ferring D., Kahlina K., Jansen R.P.;
RT "Characterization of Candida albicans ASH1 in Saccharomyces cerevisiae.";
RL Curr. Genet. 41:73-81(2002).
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, AND SUBCELLULAR LOCATION.
RX PubMed=12446785; DOI=10.1128/mcb.22.24.8669-8680.2002;
RA Inglis D.O., Johnson A.D.;
RT "Ash1 protein, an asymmetrically localized transcriptional regulator,
RT controls filamentous growth and virulence of Candida albicans.";
RL Mol. Cell. Biol. 22:8669-8680(2002).
RN [6]
RP INDUCTION.
RX PubMed=16998073; DOI=10.1128/ec.00155-06;
RA Mulhern S.M., Logue M.E., Butler G.;
RT "Candida albicans transcription factor Ace2 regulates metabolism and is
RT required for filamentation in hypoxic conditions.";
RL Eukaryot. Cell 5:2001-2013(2006).
RN [7]
RP SUBCELLULAR LOCATION, AND MRNA TRANSPORT.
RX PubMed=19779551; DOI=10.1371/journal.pgen.1000664;
RA Elson S.L., Noble S.M., Solis N.V., Filler S.G., Johnson A.D.;
RT "An RNA transport system in Candida albicans regulates hyphal morphology
RT and invasive growth.";
RL PLoS Genet. 5:E1000664-E1000664(2009).
CC -!- FUNCTION: Component of the RPD3C(L) histone deacetylase complex (HDAC).
CC Responsible for the deacetylation of lysine residues on the N-terminal
CC part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation
CC gives a tag for epigenetic repression and plays an important role in
CC transcriptional regulation, cell cycle progression and developmental
CC events (By similarity). Controls filamentous growth and required for
CC full virulence in a mouse model of disseminated candidiasis.
CC {ECO:0000250, ECO:0000269|PubMed:12073088,
CC ECO:0000269|PubMed:12446785}.
CC -!- SUBUNIT: Component of the RPD3C(L) complex. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:12073088,
CC ECO:0000269|PubMed:12446785, ECO:0000269|PubMed:19779551}.
CC Note=Preferentially accumulates in daughter cell nuclei of budding-
CC yeast form to the hyphal tip cells in growing filaments.
CC -!- INDUCTION: Expression is controlled by the transcription factor ACE2.
CC {ECO:0000269|PubMed:16998073}.
CC -!- DISRUPTION PHENOTYPE: Leads to defects in responding to some filament-
CC inducing conditions and to reduced virulence.
CC {ECO:0000269|PubMed:12446785}.
CC -!- MISCELLANEOUS: The ASH1 mRNA is transported to the daughter cell before
CC cytokinesis where translation produces the protein to block mating-type
CC switching, as well as to the hyphal tips during filamentous growth.
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DR EMBL; CP017624; AOW28034.1; -; Genomic_DNA.
DR RefSeq; XP_717146.1; XM_712053.1.
DR AlphaFoldDB; Q5A5Q6; -.
DR STRING; 237561.Q5A5Q6; -.
DR PRIDE; Q5A5Q6; -.
DR GeneID; 3641249; -.
DR KEGG; cal:CAALFM_C210660WA; -.
DR CGD; CAL0000186708; ASH1.
DR VEuPathDB; FungiDB:C2_10660W_A; -.
DR eggNOG; ENOG502QX5C; Eukaryota.
DR HOGENOM; CLU_027584_0_0_1; -.
DR InParanoid; Q5A5Q6; -.
DR OMA; FIPITPP; -.
DR OrthoDB; 1044486at2759; -.
DR PRO; PR:Q5A5Q6; -.
DR Proteomes; UP000000559; Chromosome 2.
DR GO; GO:0005634; C:nucleus; IDA:CGD.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IGI:CGD.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0009267; P:cellular response to starvation; IMP:CGD.
DR GO; GO:0006325; P:chromatin organization; IEA:UniProtKB-KW.
DR GO; GO:0030447; P:filamentous growth; IMP:CGD.
DR GO; GO:0044182; P:filamentous growth of a population of unicellular organisms; IMP:CGD.
DR GO; GO:0036180; P:filamentous growth of a population of unicellular organisms in response to biotic stimulus; IMP:CGD.
DR GO; GO:0036178; P:filamentous growth of a population of unicellular organisms in response to neutral pH; IMP:CGD.
DR GO; GO:0036170; P:filamentous growth of a population of unicellular organisms in response to starvation; IMP:CGD.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IGI:CGD.
DR GO; GO:1900430; P:positive regulation of filamentous growth of a population of unicellular organisms; IMP:CGD.
DR GO; GO:1900445; P:positive regulation of filamentous growth of a population of unicellular organisms in response to biotic stimulus; IMP:CGD.
DR GO; GO:1900442; P:positive regulation of filamentous growth of a population of unicellular organisms in response to neutral pH; IMP:CGD.
DR GO; GO:1900436; P:positive regulation of filamentous growth of a population of unicellular organisms in response to starvation; IMP:CGD.
DR GO; GO:0036176; P:response to neutral pH; IMP:CGD.
DR CDD; cd00202; ZnF_GATA; 1.
DR Gene3D; 3.30.50.10; -; 1.
DR InterPro; IPR000679; Znf_GATA.
DR InterPro; IPR013088; Znf_NHR/GATA.
DR Pfam; PF00320; GATA; 1.
DR SMART; SM00401; ZnF_GATA; 1.
DR PROSITE; PS50114; GATA_ZN_FINGER_2; 1.
PE 2: Evidence at transcript level;
KW Chromatin regulator; DNA-binding; Metal-binding; Nucleus;
KW Reference proteome; Repressor; Transcription; Transcription regulation;
KW Virulence; Zinc; Zinc-finger.
FT CHAIN 1..449
FT /note="Transcriptional regulatory protein ASH1"
FT /id="PRO_0000422798"
FT ZN_FING 365..391
FT /note="GATA-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00094"
FT REGION 48..67
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 83..111
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 256..361
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 262..283
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 289..331
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 449 AA; 50418 MW; 35807B5E622E1A1A CRC64;
MSLVQSAMFN HHMGPSHTRS RSFELFQEAK TLRVAACKRS YSDDLNISTK RARTAPPSPP
YETATPTRAS RLVISNLVKP TSNLRSRSLS PNKKFSSPLS PSSSPIATVT SPISSPQNIK
IKLPSISDAL NLPPITSKEP VKLKPIVPTV SLDYFDTYKP NDENWRYGLL DKIAKESKHF
HLNQYNYLND HAKPSFDSKL SSKIQQSSIA NGKKINFPYE SNYTYLNKTY LNDVKNYPEY
LELAAESLLQ LKERQLPPPP LQSHQKQHPP PPPHLPVQGL LPVPAPPVSH FSSSYTTPQA
PTFYHPPSQQ QQQTPPPQTL HHQQPQPPIS THKFIPISPP SAKKSRSDLL KSPPKHHHHH
APRVCISCGS DQSPCWRPSW SIKQGQLCNS CGLRYKKTKA RCLNDKCKKI PAKGEWTLMQ
SKGKETFEDG IEGYSCIACG WRVEVLPKS
