ID   P4B_VACCC               Reviewed;         644 AA.
AC   P20643;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1991, sequence version 1.
DT   03-AUG-2022, entry version 64.
DE   RecName: Full=Major core protein 4b;
DE   AltName: Full=Virion core protein 4b;
DE            Short=p4b;
DE   Flags: Precursor;
GN   ORFNames=A3L;
OS   Vaccinia virus (strain Copenhagen) (VACV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=10249;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=2219722; DOI=10.1016/0042-6822(90)90294-2;
RA   Goebel S.J., Johnson G.P., Perkus M.E., Davis S.W., Winslow J.P.,
RA   Paoletti E.;
RT   "The complete DNA sequence of vaccinia virus.";
RL   Virology 179:247-266(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Goebel S.J., Johnson G.P., Perkus M.E., Davis S.W., Winslow J.P.,
RA   Paoletti E.;
RT   "Appendix to 'The complete DNA sequence of vaccinia virus'.";
RL   Virology 179:517-563(1990).
CC   -!- FUNCTION: Major component of the virion core that undergoes proteolytic
CC       processing during the immature virion (IV) to mature virion (MV)
CC       transition. Essential for the formation of a structurally normal core
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000250}. Note=Localizes to the
CC       virion core wall, the mature protein accounts for 11% of the dry mass
CC       of the virion. {ECO:0000250}.
CC   -!- PTM: The 73-kDa precursor is cleaved to a mature protein of 60 kDa
CC       during virion maturation. Proteolytic cleavage of major core proteins
CC       P4a (A10L), P4b (A3L), and VP8 (L4R), which occurs at a late stage of
CC       core formation, is required for production of infectious mature virions
CC       (MV) (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the poxviridae protein P4b family.
CC       {ECO:0000305}.
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DR   EMBL; M35027; AAA48118.1; -; Genomic_DNA.
DR   PIR; E42517; FOVZ5R.
DR   PRIDE; P20643; -.
DR   Proteomes; UP000008269; Genome.
DR   InterPro; IPR004972; P4B.
DR   Pfam; PF03292; Pox_P4B; 1.
PE   3: Inferred from homology;
KW   Reference proteome; Virion.
FT   PROPEP          1..61
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000040581"
FT   CHAIN           62..644
FT                   /note="Major core protein 4b"
FT                   /id="PRO_0000040582"
SQ   SEQUENCE   644 AA;  72625 MW;  76CC2ED6781F88A2 CRC64;
     MEAVVNSDVF LTSNAGLKSS YTNQTLSLVD EDHIHTSDKS LSCSVCNSLS QIVDDDFISA
     GARNQRTKPK RAGNNQSQQP IKKDCMVSID EVASTHDWST RLRNDGNAIA KYLTTNKYDT
     SNFTIQDMLN IMNKLNIVRT NRNELFQLLT HVKSTLNNAS VSVKCTHPLV LIHSRASPRI
     GDQLKELDKI YSPSNHHILL STTRFQSMHF TDMSSSQDLS FIYRKPETNY YIHPILMALF
     GIKLPALENA YVHGDTYSLI QQLYEFRKVK SYNYMLLVNR LTEDNPIVIT GVSDLISTEI
     QRANMHTMIR KAIMNIRMGI FYCNDDDAVD PHLMKIIHTG CSQVMTDEEQ ILASILSIVG
     FRPTLVSVAR PINGISYDMK LQAAPYIVVN PMKMITTSDS PISINSKDIY SMAFDGNSGR
     VVFAPPNIGY GRCSGVTHID PLGTNVMGSA VHSPVIVNGA MMFYVERRQN KNMFGGECYT
     GFRSLIDDTP IDVSPEIMLN GIMYRLKSAV CYKLGDQFFD CGSSDIFLKG HYTILFTENG
     PWMYDPLSVF NPGARNARLM RALKNQYKKL SMDSDDGFYE WLNGDGSVFA ASKQQMLMNH
     VANFDDDLLT MEEAMSMISR HCCILIYAQD YDQYISARHI TELF