ID   P4B_VACCW               Reviewed;         643 AA.
AC   P06440; Q76ZR0;
DT   01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1988, sequence version 1.
DT   29-SEP-2021, entry version 69.
DE   RecName: Full=Major core protein 4b;
DE   AltName: Full=Virion core protein 4b;
DE            Short=p4b;
DE   Flags: Precursor;
GN   OrderedLocusNames=VACWR122; ORFNames=A3L;
OS   Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain
OS   WR)).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=10254;
OH   NCBI_TaxID=9913; Bos taurus (Bovine).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2999438; DOI=10.1128/jvi.56.3.830-838.1985;
RA   Rosel J., Moss B.;
RT   "Transcriptional and translational mapping and nucleotide sequence analysis
RT   of a vaccinia virus gene encoding the precursor of the major core
RT   polypeptide 4b.";
RL   J. Virol. 56:830-838(1985).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J.,
RA   Wohlhueter R.;
RT   "Sequencing of the coding region of Vaccinia-WR to an average 9-fold
RT   redundancy and an error rate of 0.16/10kb.";
RL   Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-201.
RA   van Meir E.;
RL   Submitted (SEP-1989) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   PROTEIN SEQUENCE OF 62-70.
RX   PubMed=1993877; DOI=10.1099/0022-1317-72-2-411;
RA   van Slyke J.K., Franke C.A., Hruby D.E.;
RT   "Proteolytic maturation of vaccinia virus core proteins: identification of
RT   a conserved motif at the N termini of the 4b and 25K virion proteins.";
RL   J. Gen. Virol. 72:411-416(1991).
CC   -!- FUNCTION: Major component of the virion core that undergoes proteolytic
CC       processing during the immature virion (IV) to mature virion (MV)
CC       transition. Essential for the formation of a structurally normal core.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000305}. Note=Localizes to the
CC       virion core wall, the mature protein accounts for 11% of the dry mass
CC       of the virion.
CC   -!- PTM: The 73-kDa precursor is cleaved to a mature protein of 60 kDa
CC       during virion maturation. Proteolytic cleavage of major core proteins
CC       P4a (A10L), P4b (A3L), and VP8 (L4R), which occurs at a late stage of
CC       core formation, is required for production of infectious mature virions
CC       (MV).
CC   -!- SIMILARITY: Belongs to the poxviridae protein P4b family.
CC       {ECO:0000305}.
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DR   EMBL; M11079; AAA48298.1; -; Genomic_DNA.
DR   EMBL; AY243312; AAO89401.1; -; Genomic_DNA.
DR   EMBL; M27914; AAA48303.1; -; Genomic_DNA.
DR   PIR; A03871; FOVZZW.
DR   IntAct; P06440; 1.
DR   MINT; P06440; -.
DR   Proteomes; UP000000344; Genome.
DR   InterPro; IPR004972; P4B.
DR   Pfam; PF03292; Pox_P4B; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Reference proteome; Virion.
FT   PROPEP          1..61
FT                   /evidence="ECO:0000269|PubMed:1993877"
FT                   /id="PRO_0000040583"
FT   CHAIN           62..643
FT                   /note="Major core protein 4b"
FT                   /id="PRO_0000040584"
FT   CONFLICT        122
FT                   /note="Q -> N (in Ref. 3; AAA48303 and 2; AAO89401)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        317
FT                   /note="I -> R (in Ref. 2; AAO89401)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        545
FT                   /note="D -> DP (in Ref. 2; AAO89401)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   643 AA;  72499 MW;  6F073332BAFD0E59 CRC64;
     MEAVVNSDVF LTSNAGLKSS YTNQTLSLVD EDHIHTSDKS LSCSVCNSLS QIVDDDFISA
     GARNQRTKPK RAGNNQSQQP IKKDCMVSID EVASTHDWST RLRNDGNAIA KYLTTNKYDT
     SQFTIQDMLN IMNKLNIVRT NRNELFQLLT HVKSTLNNAS VSVKCTHPLV LIHSRASPRI
     GDQLKELDKI YSPSNHHILL STTRFQSMHF TDMSSSQDLS FIYRKPETNY YIHPILMALF
     GIKLPALENA YVHGDTYSLI QQLYEFRKVK SYNYMLLVNR LTEDNPIVIT GVSDLISTEI
     QRANMHTMIR KAIMNIIMGI FYCNDDDAVD PHLMKIIHTG CSQVMTDEEQ ILASILSIVG
     FRPTLVSVAR PINGISYDMK LQAAPYIVVN PMKMITTSDS PISINSKDIY SMAFDGNSGR
     VVFAPPNIGY GRCSGVTHID PLGTNVMGSA VHSPVIVNGA MMFYVERRQN KNMFGGECYT
     GFRSLIDDTP IDVSPEIMLN GIMYRLKSAV CYKLGDQFFD CGSSDIFLKG HYTILFTENG
     PWMYDLSVFN PGARNARLMR ALKNQYKKLS MDSDDGFYEW LNGDGSVFAA SKQQMLMNHV
     ANFDDDLLTM EEAMSMISRH CCILIYAQDY DQYISARHIT ELF