ID   P4B_VARV                Reviewed;         644 AA.
AC   P0DOM6; P33818;
DT   16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT   16-OCT-2019, sequence version 1.
DT   29-SEP-2021, entry version 10.
DE   RecName: Full=Major core protein 4b;
DE   AltName: Full=Virion core protein 4b;
DE            Short=p4b;
DE   Flags: Precursor;
GN   ORFNames=A3L;
OS   Variola virus.
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus.
OX   NCBI_TaxID=10255;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Bangladesh-1975;
RX   PubMed=8264798; DOI=10.1038/366748a0;
RA   Massung R.F., Esposito J.J., Liu L.I., Qi J., Utterback T.R., Knight J.C.,
RA   Aubin L., Yuran T.E., Parsons J.M., Loparev V.N., Selivanov N.A.,
RA   Cavallaro K.F., Kerlavage A.R., Mahy B.W.J., Venter J.C.;
RT   "Potential virulence determinants in terminal regions of variola smallpox
RT   virus genome.";
RL   Nature 366:748-751(1993).
CC   -!- FUNCTION: Major component of the virion core that undergoes proteolytic
CC       processing during the immature virion (IV) to mature virion (MV)
CC       transition. Essential for the formation of a structurally normal core
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000250}. Note=Localizes to the
CC       virion core wall, the mature protein accounts for 11% of the dry mass
CC       of the virion. {ECO:0000250}.
CC   -!- PTM: The 73-kDa precursor is cleaved to a mature protein of 60 kDa
CC       during virion maturation. Proteolytic cleavage of major core proteins
CC       P4a (A10L), P4b (A3L), and VP8 (L4R), which occurs at a late stage of
CC       core formation, is required for production of infectious mature virions
CC       (MV) (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the poxviridae protein P4b family.
CC       {ECO:0000305}.
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DR   EMBL; L22579; AAA60855.1; -; Genomic_DNA.
DR   PIR; T28545; T28545.
DR   RefSeq; NP_042151.1; NC_001611.1.
DR   GeneID; 1486519; -.
DR   KEGG; vg:1486519; -.
DR   Proteomes; UP000119805; Genome.
DR   InterPro; IPR004972; P4B.
DR   Pfam; PF03292; Pox_P4B; 1.
PE   3: Inferred from homology;
KW   Virion.
FT   PROPEP          1..61
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000448145"
FT   CHAIN           62..644
FT                   /note="Major core protein 4b"
FT                   /id="PRO_0000448146"
FT   REGION          61..80
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   644 AA;  72650 MW;  24483D2A520BC2D1 CRC64;
     MEAVVNSDVF LTSNTGLKSS YTNQTLSLVD EDHIHTSDKS LSCSVCNSLS QIVDDDFISA
     GARNQRTKPK RAGNDQAQQT TKKDCMVSID EVASTHDWST RLRNDGNAIA KYLTTNKYDT
     SNFTIQDMLN IMNKLNIVRT NRNELFQLLT HVKSTLNNAS VSVKCTHPLV LIHSRASPRI
     GDQLKELDKI YSPSNHHILL STTRFQSMHF TDMSSSQDLS FIYRKPETNY YIHPILMALF
     GIKLPALENA YVHGDTYSLI QQLYEFRRVK SYNYMLLVNR LTEDNPIVIT GVSDLISTEI
     QRANMHTMIR KAIMNIRMGI FYCNDDDAVD PHLMKIIHTG CSQVMTDEEQ ILASILSIVG
     FRPTLVSVAR PINGISYDMK LQAAPYIVVN PMKMITTSDS PISINSKDIY SMAFDGNSGR
     VVFAPPNIGY GRCSGVTHID SLGTNVMGSA VHSPVIVNGA MMFYVERRQN KNMFGGECYT
     GFRSLIDDTP IDVSPEIMLN GIMYRLKSAV CYKLGDQFFD CGSSDIFLKG HYTILFTENG
     PWMYDPLSVF NPGARNARLM RALKNQYKKL SMDSDDGFYE WLNGDGSVFA ASKQQMLMNH
     VANFDDDLLT MEEAMSMISR HCCILIYAQD YDQYISARHI TELF