P4KA2_ARATH
ID P4KA2_ARATH Reviewed; 525 AA.
AC Q9C680;
DT 05-OCT-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Phosphatidylinositol 4-kinase alpha 2;
DE Short=PI4-kinase alpha 2;
DE Short=PtdIns-4-kinase alpha 2;
DE EC=2.7.1.67;
DE AltName: Full=Phosphatidylinositol 4-OH kinase alpha2;
DE Short=AtPI4Kalpha2;
DE Short=PI-4Kalpha2;
GN Name=PI4KA2; Synonyms=PI4KALPHA2; OrderedLocusNames=At1g51040;
GN ORFNames=F23H24.10;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12226484; DOI=10.1104/pp.004770;
RA Mueller-Roeber B., Pical C.;
RT "Inositol phospholipid metabolism in Arabidopsis. Characterized and
RT putative isoforms of inositol phospholipid kinase and phosphoinositide-
RT specific phospholipase C.";
RL Plant Physiol. 130:22-46(2002).
CC -!- FUNCTION: Acts on phosphatidylinositol (PtdIns) in the first committed
CC step in the production of the second messenger inositol-1,4,5,-
CC trisphosphate. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + ATP = a
CC 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate) + ADP +
CC H(+); Xref=Rhea:RHEA:19877, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:57880, ChEBI:CHEBI:58178, ChEBI:CHEBI:456216;
CC EC=2.7.1.67;
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Peripheral membrane
CC protein {ECO:0000305}; Cytoplasmic side {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the PI3/PI4-kinase family. Type III PI4K
CC subfamily. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AC079828; AAG50530.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE32615.1; -; Genomic_DNA.
DR PIR; F96547; F96547.
DR RefSeq; NP_175516.1; NM_103983.2.
DR AlphaFoldDB; Q9C680; -.
DR SMR; Q9C680; -.
DR STRING; 3702.AT1G51040.1; -.
DR PaxDb; Q9C680; -.
DR PRIDE; Q9C680; -.
DR EnsemblPlants; AT1G51040.1; AT1G51040.1; AT1G51040.
DR GeneID; 841525; -.
DR Gramene; AT1G51040.1; AT1G51040.1; AT1G51040.
DR KEGG; ath:AT1G51040; -.
DR Araport; AT1G51040; -.
DR TAIR; locus:2026063; AT1G51040.
DR eggNOG; KOG0902; Eukaryota.
DR HOGENOM; CLU_011483_1_0_1; -.
DR InParanoid; Q9C680; -.
DR OMA; CEEDILM; -.
DR OrthoDB; 1147978at2759; -.
DR PhylomeDB; Q9C680; -.
DR BioCyc; ARA:AT1G51040-MON; -.
DR PRO; PR:Q9C680; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9C680; baseline and differential.
DR Genevisible; Q9C680; AT.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0004430; F:1-phosphatidylinositol 4-kinase activity; ISS:UniProtKB.
DR GO; GO:0048015; P:phosphatidylinositol-mediated signaling; IEA:InterPro.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR Gene3D; 1.10.1070.11; -; 1.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000403; PI3/4_kinase_cat_dom.
DR InterPro; IPR036940; PI3/4_kinase_cat_sf.
DR InterPro; IPR018936; PI3/4_kinase_CS.
DR InterPro; IPR015433; PI_Kinase.
DR PANTHER; PTHR10048; PTHR10048; 2.
DR Pfam; PF00454; PI3_PI4_kinase; 1.
DR SMART; SM00146; PI3Kc; 1.
DR SUPFAM; SSF56112; SSF56112; 1.
DR PROSITE; PS00915; PI3_4_KINASE_1; 1.
DR PROSITE; PS50290; PI3_4_KINASE_3; 1.
PE 3: Inferred from homology;
KW Kinase; Membrane; Reference proteome; Transferase.
FT CHAIN 1..525
FT /note="Phosphatidylinositol 4-kinase alpha 2"
FT /id="PRO_0000398593"
FT DOMAIN 239..509
FT /note="PI3K/PI4K catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00269"
FT REGION 163..278
FT /note="Pleckstrin homology (PH) domain conferring
FT phosphoinositide binding specificity"
FT /evidence="ECO:0000250"
FT REGION 245..251
FT /note="G-loop"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00269"
FT REGION 373..381
FT /note="Catalytic loop"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00269"
FT REGION 392..417
FT /note="Activation loop"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00269"
SQ SEQUENCE 525 AA; 59290 MW; 1316625BDF7ECAB8 CRC64;
MERLNELCDI IEQNPKQFLD KLPWICQQCP QSKLLRAESP RFSQSHLNAI LAVTRILSKI
VDTTDENAKF VVLDFLQTVP KSFHRSFWPY SLSLESISAF YCSFLGYVSC LSQLMVEGYL
LRAAQRSDIF AHTLIWHLQG ESVEEIVKDG AFDKNASFQE ILSDVRQHIV DGFTPKALNF
FSREFDFFEK VTSISGALLP LPKEERVAGI RRELEKIKMQ GEDLYLPTAP NKLVRVIQVD
SGIPLQSAAK VPIMITFNVV DLDGDHNDVK PQACIFKVGD DCRQDVLALQ VISLLGDIFQ
AVGLNLYLFP YGVLPTVVPN TRSRSQMGET TDGGLYEIFQ QNYGLVGSTT FETARANFLI
SSAGYAVASL LLQPKDRHNG NLLFDDVGRL VHIDFGFILE TSPGGNMRFE NAHFKLSHEM
TQLLDPSGVM KSKTWHQFVS LCVKGYLAAR RYMDEIISTV QMMLESGLPC FSRGDPIGNL
RKRFHPEMSE REAALFMINV CTDAYNKWTT AGYDLIQYLQ QGVEK
