P4KG6_ARATH
ID P4KG6_ARATH Reviewed; 622 AA.
AC Q8W4R8; Q9LMY3;
DT 18-SEP-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Phosphatidylinositol 4-kinase gamma 6;
DE Short=AtPI4Kgamma6;
DE Short=PI-4Kgamma6;
DE Short=PI4K gamma 6;
DE EC=2.7.1.67;
GN Name=PI4KG6; Synonyms=PI4KGAMMA6; OrderedLocusNames=At1g13640;
GN ORFNames=F21F23.8;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12226484; DOI=10.1104/pp.004770;
RA Mueller-Roeber B., Pical C.;
RT "Inositol phospholipid metabolism in Arabidopsis. Characterized and
RT putative isoforms of inositol phospholipid kinase and phosphoinositide-
RT specific phospholipase C.";
RL Plant Physiol. 130:22-46(2002).
RN [5]
RP GENE FAMILY.
RX PubMed=17880284; DOI=10.1042/bj20070959;
RA Galvao R.M., Kota U., Soderblom E.J., Goshe M.B., Boss W.F.;
RT "Characterization of a new family of protein kinases from Arabidopsis
RT containing phosphoinositide 3/4-kinase and ubiquitin-like domains.";
RL Biochem. J. 409:117-127(2008).
CC -!- FUNCTION: The phosphorylation of phosphatidylinositol (PI) to PI4P is
CC the first committed step in the generation of phosphatidylinositol 4,5-
CC bisphosphate (PIP2), a precursor of the second messenger inositol
CC 1,4,5-trisphosphate (InsP3). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + ATP = a
CC 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate) + ADP +
CC H(+); Xref=Rhea:RHEA:19877, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:57880, ChEBI:CHEBI:58178, ChEBI:CHEBI:456216;
CC EC=2.7.1.67;
CC -!- SIMILARITY: Belongs to the PI3/PI4-kinase family. Type II PI4K
CC subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF81291.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AC027656; AAF81291.1; ALT_INIT; Genomic_DNA.
DR EMBL; CP002684; AEE29053.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM59920.1; -; Genomic_DNA.
DR EMBL; AY060574; AAL31202.1; -; mRNA.
DR EMBL; AY149944; AAN31098.1; -; mRNA.
DR PIR; F86269; F86269.
DR RefSeq; NP_001322238.1; NM_001332079.1.
DR RefSeq; NP_563930.1; NM_101234.4.
DR AlphaFoldDB; Q8W4R8; -.
DR SMR; Q8W4R8; -.
DR STRING; 3702.AT1G13640.1; -.
DR iPTMnet; Q8W4R8; -.
DR PaxDb; Q8W4R8; -.
DR PRIDE; Q8W4R8; -.
DR ProteomicsDB; 250814; -.
DR EnsemblPlants; AT1G13640.1; AT1G13640.1; AT1G13640.
DR EnsemblPlants; AT1G13640.2; AT1G13640.2; AT1G13640.
DR GeneID; 837925; -.
DR Gramene; AT1G13640.1; AT1G13640.1; AT1G13640.
DR Gramene; AT1G13640.2; AT1G13640.2; AT1G13640.
DR KEGG; ath:AT1G13640; -.
DR Araport; AT1G13640; -.
DR TAIR; locus:2023865; AT1G13640.
DR eggNOG; KOG2381; Eukaryota.
DR HOGENOM; CLU_027241_1_1_1; -.
DR InParanoid; Q8W4R8; -.
DR OMA; DNDGFTQ; -.
DR OrthoDB; 1273723at2759; -.
DR PhylomeDB; Q8W4R8; -.
DR PRO; PR:Q8W4R8; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8W4R8; baseline and differential.
DR Genevisible; Q8W4R8; AT.
DR GO; GO:0004430; F:1-phosphatidylinositol 4-kinase activity; IEA:UniProtKB-EC.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR InterPro; IPR044571; P4KG1-8.
DR InterPro; IPR000403; PI3/4_kinase_cat_dom.
DR InterPro; IPR029071; Ubiquitin-like_domsf.
DR PANTHER; PTHR45800; PTHR45800; 1.
DR Pfam; PF00454; PI3_PI4_kinase; 1.
DR SUPFAM; SSF54236; SSF54236; 1.
DR PROSITE; PS50290; PI3_4_KINASE_3; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Kinase; Nucleotide-binding; Phosphoprotein;
KW Reference proteome; Transferase.
FT CHAIN 1..622
FT /note="Phosphatidylinositol 4-kinase gamma 6"
FT /id="PRO_0000423363"
FT DOMAIN 38..95
FT /note="Ubiquitin-like; degenerate"
FT DOMAIN 158..459
FT /note="PI3K/PI4K catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00269"
FT REGION 164..170
FT /note="G-loop"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00269"
FT REGION 310..318
FT /note="Catalytic loop"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00269"
FT REGION 339..365
FT /note="Activation loop"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00269"
FT BINDING 165..171
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250|UniProtKB:Q9BTU6"
FT BINDING 186
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250|UniProtKB:Q9BTU6"
FT BINDING 277..280
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250|UniProtKB:Q9BTU6"
FT BINDING 341
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250|UniProtKB:Q9BTU6"
FT MOD_RES 565
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9C671"
SQ SEQUENCE 622 AA; 69572 MW; E62960FD1881256A CRC64;
MAMAVFKAPL KGEFHGARKM EGKQYKHHLL QQQSTGRRRV FVQTDTGCVL GVELDRNDNV
HTVKKRLQIA FNFPTEESSL TFGDMVLKND LSAVRNDSPL LLKRNLMHRS SSTPCLSPTG
NDLQRKDRSG PIEILSHSPC FLSLKQTAND IVKAMKMGVE PIPVNGGLGG AYYFRDEKGQ
SVAIVKPTDE EPFAPNNPKG FVGKALGQPG LKPSVRVGET GFREVAAYLL DYDHFANVPP
TALVKITHSV FNVNDGMDGN KSREKKKLVS SKIASFQKFV PHDFDASDHG TSSFPVASVH
RIGILDIRIL NTDRHGGNLL VKKLDDGGVG RFGQVELIPI DHGLCLPETL EDPYFEWIHW
PQASIPFSEE ELDYIQSLDP VKDCEMLRRE LPMIREACLR VLVLCTVFLK EAAVFGLCLA
EIGEMMTREF RAGEEEPSEL EMLCIEAKRL TTEQDVLSPK SDGEGETEFQ FDIDYNELDS
VYGSETETDE FFAKNPFSNG RSSLGELKES IAEEEEDDEE EAKLTLSLSK LSTSMKNNLS
NTMGSGYLKP PKDNQTDKAL VSHKSANVQL PLSVNFVKLA DMKEVEWVVF LERFQELLYS
AFAERKTMTL RNTQRLGTSC KF
