ID   A30_VACCW               Reviewed;          77 AA.
AC   P68596; P21088; Q76ZP5;
DT   07-DEC-2004, integrated into UniProtKB/Swiss-Prot.
DT   07-DEC-2004, sequence version 1.
DT   25-MAY-2022, entry version 34.
DE   RecName: Full=Protein A30;
GN   OrderedLocusNames=VACWR153; ORFNames=A30L;
OS   Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain
OS   WR)).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=10254;
OH   NCBI_TaxID=9913; Bos taurus (Bovine).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1856205; DOI=10.1016/s0021-9258(18)92757-2;
RA   Amegadzie B.Y., Ahn B.-Y., Moss B.;
RT   "Identification, sequence, and expression of the gene encoding a Mr 35,000
RT   subunit of the vaccinia virus DNA-dependent RNA polymerase.";
RL   J. Biol. Chem. 266:13712-13718(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J.,
RA   Wohlhueter R.;
RT   "Sequencing of the coding region of Vaccinia-WR to an average 9-fold
RT   redundancy and an error rate of 0.16/10kb.";
RL   Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   FUNCTION.
RX   PubMed=11390577; DOI=10.1128/jvi.75.13.5752-5761.2001;
RA   Szajner P., Weisberg A.S., Wolffe E.J., Moss B.;
RT   "Vaccinia virus A30L protein is required for association of viral membranes
RT   with dense viroplasm to form immature virions.";
RL   J. Virol. 75:5752-5761(2001).
RN   [4]
RP   INTERACTION WITH PROTEIN G7.
RX   PubMed=12610117; DOI=10.1128/jvi.77.6.3418-3429.2003;
RA   Szajner P., Jaffe H., Weisberg A.S., Moss B.;
RT   "Vaccinia virus G7L protein interacts with the A30L protein and is required
RT   for association of viral membranes with dense viroplasm to form immature
RT   virions.";
RL   J. Virol. 77:3418-3429(2003).
RN   [5]
RP   PHOSPHORYLATION.
RX   PubMed=14671108; DOI=10.1128/jvi.78.1.266-274.2004;
RA   Szajner P., Weisberg A.S., Moss B.;
RT   "Physical and functional interactions between vaccinia virus F10 protein
RT   kinase and virion assembly proteins A30 and G7.";
RL   J. Virol. 78:266-274(2004).
RN   [6]
RP   IDENTIFICATION BY MASS SPECTROMETRY.
RX   PubMed=16509968; DOI=10.1186/1743-422x-3-10;
RA   Yoder J.D., Chen T.S., Gagnier C.R., Vemulapalli S., Maier C.S.,
RA   Hruby D.E.;
RT   "Pox proteomics: mass spectrometry analysis and identification of Vaccinia
RT   virion proteins.";
RL   Virol. J. 3:10-10(2006).
RN   [7]
RP   INTERACTION WITH PROTEIN F17.
RX   PubMed=20392848; DOI=10.1128/jvi.00399-10;
RA   Wickramasekera N.T., Traktman P.;
RT   "Structure/Function analysis of the vaccinia virus F18 phosphoprotein, an
RT   abundant core component required for virion maturation and infectivity.";
RL   J. Virol. 84:6846-6860(2010).
CC   -!- FUNCTION: Required for the association between the dense viroplasm and
CC       the viral membranes to form the mature virion (MV).
CC       {ECO:0000269|PubMed:11390577}.
CC   -!- SUBUNIT: Interacts with protein G7; the interaction stabilizes both
CC       proteins (PubMed:12610117). Interacts with protein F17.
CC       {ECO:0000269|PubMed:12610117, ECO:0000269|PubMed:20392848}.
CC   -!- PTM: Phosphorylated by viral F10 kinase. {ECO:0000269|PubMed:14671108}.
CC   -!- SIMILARITY: Belongs to the chordopoxvirinae A30 family. {ECO:0000305}.
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DR   EMBL; M61187; AAA48327.1; -; Genomic_DNA.
DR   EMBL; AY243312; AAO89432.1; -; Genomic_DNA.
DR   RefSeq; YP_233035.1; NC_006998.1.
DR   DNASU; 3707683; -.
DR   GeneID; 3707683; -.
DR   KEGG; vg:3707683; -.
DR   Proteomes; UP000000344; Genome.
DR   InterPro; IPR009257; Chordopox_A30L.
DR   Pfam; PF06015; Chordopox_A30L; 1.
PE   1: Evidence at protein level;
KW   Phosphoprotein; Reference proteome.
FT   CHAIN           1..77
FT                   /note="Protein A30"
FT                   /id="PRO_0000099306"
FT   REGION          53..77
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   77 AA;  8745 MW;  BC30812604F804E2 CRC64;
     MEDLNEANFS HLLINLSNNK DIDAQYASTL SVVHELLSAI NFKIFNINKK SKKNSKSIEQ
     HPVVHHAASA GREFNRR