P54_ASFK5
ID P54_ASFK5 Reviewed; 175 AA.
AC P0C9Z8;
DT 05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 05-MAY-2009, sequence version 1.
DT 03-AUG-2022, entry version 43.
DE RecName: Full=Inner membrane protein p54 {ECO:0000305};
DE AltName: Full=pE183L {ECO:0000250|UniProtKB:Q65194};
GN OrderedLocusNames=Ken-138;
OS African swine fever virus (isolate Pig/Kenya/KEN-50/1950) (ASFV).
OC Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC Asfuvirales; Asfarviridae; Asfivirus.
OX NCBI_TaxID=561445;
OH NCBI_TaxID=6937; Ornithodoros (relapsing fever ticks).
OH NCBI_TaxID=85517; Phacochoerus aethiopicus (Warthog).
OH NCBI_TaxID=41426; Phacochoerus africanus (Warthog).
OH NCBI_TaxID=273792; Potamochoerus larvatus (Bushpig).
OH NCBI_TaxID=9823; Sus scrofa (Pig).
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Kutish G.F., Rock D.L.;
RT "African swine fever virus genomes.";
RL Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Inner envelope protein involved, through its interaction with
CC host dynein, in the intracellular microtubule-dependent transport of
CC viral capsid toward viral factories (By similarity). Seems to induce
CC caspase-3 activation and apoptosis (By similarity). Plays a role in
CC virion morphogenesis by recruiting and transforming the host ER
CC membranes into the precursors of the viral envelope (By similarity).
CC Involved in virus attachment to the host cell (By similarity).
CC {ECO:0000250|UniProtKB:Q65194}.
CC -!- SUBUNIT: Interacts with the host light chain cytoplasmic dynein DYNLL1;
CC this interaction is critical for intracellular microtubule-dependent
CC virus transport toward viral factories. {ECO:0000250|UniProtKB:Q65194}.
CC -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000250|UniProtKB:Q65194};
CC Single-pass membrane protein {ECO:0000250|UniProtKB:Q65194}. Host
CC cytoplasm, host cytoskeleton {ECO:0000250|UniProtKB:Q65194}. Host
CC endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q65194}.
CC Note=Localizes to the viral factory at 16 hpi. Host DYNLL1 and viral
CC p54 interact at the microtubular organizing center (By similarity).
CC Found in the inner envelope of the virus (By similarity).
CC {ECO:0000250|UniProtKB:Q65194}.
CC -!- INDUCTION: Expressed in the late phase of the viral replicative cycle.
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the asfivirus envelope protein p54 family.
CC {ECO:0000305}.
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DR EMBL; AY261360; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR SMR; P0C9Z8; -.
DR Proteomes; UP000000861; Genome.
DR GO; GO:0044167; C:host cell endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0044163; C:host cytoskeleton; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0019031; C:viral envelope; IEA:UniProtKB-KW.
DR GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0039701; P:microtubule-dependent intracellular transport of viral material towards cell periphery; IEA:UniProtKB-KW.
DR InterPro; IPR008385; ASFV_p54.
DR Pfam; PF05568; ASFV_J13L; 1.
PE 3: Inferred from homology;
KW Apoptosis; Host cytoplasm; Host cytoskeleton; Host endoplasmic reticulum;
KW Host membrane; Host-virus interaction; Membrane;
KW Microtubular outwards viral transport; Transmembrane; Transmembrane helix;
KW Viral envelope protein; Viral release from host cell; Virion.
FT CHAIN 1..175
FT /note="Inner membrane protein p54"
FT /id="PRO_0000373416"
FT TRANSMEM 32..52
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 79..121
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 141..153
FT /note="Interaction with host DYNLL1"
FT /evidence="ECO:0000250"
FT COMPBIAS 79..96
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 175 AA; 18929 MW; 3A2B17D50822C898 CRC64;
MDSEFFQPVY PRHYGECLSP TSTPSFFSTH MCTILVAIVV LIIIIIVLIY LFSSRKKKAA
APAIEEEDIQ FINPYQDQQW AGATPQPGTS KPAGATTGNV GKPITDRPAT DRPVTNNPVT
DRLIMATGGP AAASAPSAEL YTTATTQNTA SQTMPAVEAL RQRSTYTHKD LENSL