ID   P54_ASFL6               Reviewed;         183 AA.
AC   Q65272;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 68.
DE   RecName: Full=Inner membrane protein p54 {ECO:0000250|UniProtKB:Q65194};
DE   AltName: Full=pE183L {ECO:0000250|UniProtKB:Q65194};
OS   African swine fever virus (isolate Pig/Portugal/Lis 60/1960) (ASFV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Asfuvirales; Asfarviridae; Asfivirus.
OX   NCBI_TaxID=82815;
OH   NCBI_TaxID=6937; Ornithodoros (relapsing fever ticks).
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND SUBCELLULAR LOCATION.
RX   PubMed=7730797; DOI=10.1099/0022-1317-76-5-1117;
RA   Sun H., Jacobs S.C., Smith G.L., Dixon L.K., Parkhouse R.M.E.;
RT   "African swine fever virus gene j13L encodes a 25-27KDa protein with
RT   variable numbers of amino acid repeats which is present in extracellular
RT   virions.";
RL   J. Gen. Virol. 76:1117-1127(1995).
RN   [2]
RP   SUBCELLULAR LOCATION.
RX   PubMed=9603333; DOI=10.1099/0022-1317-79-5-1179;
RA   Brookes S.M., Sun H., Dixon L.K., Parkhouse R.M.E.;
RT   "Characterization of African swine fever virion proteins j5R and j13L:
RT   immuno-localization in virus particles and assembly sites.";
RL   J. Gen. Virol. 79:1179-1188(1998).
CC   -!- FUNCTION: Inner envelope protein involved, through its interaction with
CC       host dynein, in the intracellular microtubule-dependent transport of
CC       viral capsid toward viral factories (By similarity). Seems to induce
CC       caspase-3 activation and apoptosis (By similarity). Plays a role in
CC       virion morphogenesis by recruiting and transforming the host ER
CC       membranes into the precursors of the viral envelope (By similarity).
CC       Involved in virus attachment to the host cell (By similarity).
CC       {ECO:0000250|UniProtKB:Q65194}.
CC   -!- SUBUNIT: Interacts with the host light chain cytoplasmic dynein DYNLL1;
CC       this interaction is critical for intracellular microtubule-dependent
CC       virus transport toward viral factories. {ECO:0000250|UniProtKB:Q65194}.
CC   -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000250|UniProtKB:Q65194};
CC       Single-pass membrane protein {ECO:0000250|UniProtKB:Q65194}. Host
CC       cytoplasm, host cytoskeleton {ECO:0000250|UniProtKB:Q65194}. Host
CC       endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q65194}.
CC       Note=Detected mainly on membrane-like structures within viral
CC       factories. Present in mature extracellular virions. Host DYNLL1 and
CC       viral p54 interact at the microtubular organizing center (By
CC       similarity). Found in the inner envelope of the virus (By similarity).
CC       {ECO:0000250|UniProtKB:Q65194}.
CC   -!- INDUCTION: Expressed in the late phase of the viral replicative cycle.
CC       {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the asfivirus envelope protein p54 family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; X84889; CAA59315.1; -; Genomic_DNA.
DR   PIR; S52904; S52904.
DR   GO; GO:0044167; C:host cell endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0044163; C:host cytoskeleton; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0019031; C:viral envelope; IEA:UniProtKB-KW.
DR   GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0075521; P:microtubule-dependent intracellular transport of viral material towards nucleus; IEA:UniProtKB-KW.
DR   GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR   InterPro; IPR008385; ASFV_p54.
DR   Pfam; PF05568; ASFV_J13L; 1.
PE   3: Inferred from homology;
KW   Apoptosis; Cytoplasmic inwards viral transport; Host cytoplasm;
KW   Host cytoskeleton; Host endoplasmic reticulum; Host membrane;
KW   Host-virus interaction; Late protein; Membrane;
KW   Microtubular inwards viral transport; Transmembrane; Transmembrane helix;
KW   Viral envelope protein; Virion; Virus entry into host cell.
FT   CHAIN           1..183
FT                   /note="Inner membrane protein p54"
FT                   /id="PRO_0000373417"
FT   TRANSMEM        32..52
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          81..157
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          149..161
FT                   /note="Interaction with host DYNLL1"
FT                   /evidence="ECO:0000250"
FT   COMPBIAS        81..95
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   183 AA;  19853 MW;  BB2AA8B987C9C7B2 CRC64;
     MDSEFFQPVY PRHYGECLSP TSTPSFFSTH MYTILIAIVV LVIIIIVLIY LFSSRKKKAA
     AAIEEEDIQF INPYQDQQWA EVTPQPGTSK PAGATTASAG KPVTGRPATN RPATNKPVTD
     NPVTDRLVMA TGGPAAAPAA ASAHPTEPYT TVTTQNTASQ TMSAIENLRQ RNTYTHKDLE
     NSL