P54_ASFM2
ID P54_ASFM2 Reviewed; 176 AA.
AC Q89784;
DT 26-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 82.
DE RecName: Full=Inner membrane protein p54 {ECO:0000250|UniProtKB:Q65194};
DE AltName: Full=pE183L {ECO:0000250|UniProtKB:Q65194};
GN OrderedLocusNames=Mal-134; ORFNames=j13L;
OS African swine fever virus (isolate Tick/Malawi/Lil 20-1/1983) (ASFV).
OC Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC Asfuvirales; Asfarviridae; Asfivirus.
OX NCBI_TaxID=10500;
OH NCBI_TaxID=6937; Ornithodoros (relapsing fever ticks).
OH NCBI_TaxID=85517; Phacochoerus aethiopicus (Warthog).
OH NCBI_TaxID=41426; Phacochoerus africanus (Warthog).
OH NCBI_TaxID=273792; Potamochoerus larvatus (Bushpig).
OH NCBI_TaxID=9823; Sus scrofa (Pig).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8409937; DOI=10.1099/0022-1317-74-10-2125;
RA Vydelingum S., Baylis S.A., Bristow C., Smith G.L., Dixon L.K.;
RT "Duplicated genes within the variable right end of the genome of a
RT pathogenic isolate of African swine fever virus.";
RL J. Gen. Virol. 74:2125-2130(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8021596; DOI=10.1099/0022-1317-75-7-1655;
RA Dixon L.K., Twigg S.R.F., Baylis S.A., Vydelingum S., Bristow C.,
RA Hammond J.M., Smith G.L.;
RT "Nucleotide sequence of a 55 kbp region from the right end of the genome of
RT a pathogenic African swine fever virus isolate (Malawi LIL20/1).";
RL J. Gen. Virol. 75:1655-1684(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND SUBCELLULAR LOCATION.
RC STRAIN=isolate Malawi Bongera 83;
RX PubMed=7730797; DOI=10.1099/0022-1317-76-5-1117;
RA Sun H., Jacobs S.C., Smith G.L., Dixon L.K., Parkhouse R.M.E.;
RT "African swine fever virus gene j13L encodes a 25-27KDa protein with
RT variable numbers of amino acid repeats which is present in extracellular
RT virions.";
RL J. Gen. Virol. 76:1117-1127(1995).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Isolate BAN/91/1, Isolate DED/89/1, Isolate DOWA, Isolate MCH/89/1,
RC and Isolate Moz/98/1;
RA Heath L.E., Vosloo W.;
RT "Genotypic analysis of African swine fever virus.";
RL Submitted (JUL-2008) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=19009341; DOI=10.1007/s11262-008-0293-2;
RA Gallardo C., Mwaengo D.M., Macharia J.M., Arias M., Taracha E.A., Soler A.,
RA Okoth E., Martin E., Kasiti J., Bishop R.P.;
RT "Enhanced discrimination of African swine fever virus isolates through
RT nucleotide sequencing of the p54, p72, and pB602L (CVR) genes.";
RL Virus Genes 38:85-95(2009).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Kutish G.F., Rock D.L.;
RT "African swine fever virus genomes.";
RL Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP SUBCELLULAR LOCATION.
RX PubMed=9603333; DOI=10.1099/0022-1317-79-5-1179;
RA Brookes S.M., Sun H., Dixon L.K., Parkhouse R.M.E.;
RT "Characterization of African swine fever virion proteins j5R and j13L:
RT immuno-localization in virus particles and assembly sites.";
RL J. Gen. Virol. 79:1179-1188(1998).
CC -!- FUNCTION: Inner envelope protein involved, through its interaction with
CC host dynein, in the intracellular microtubule-dependent transport of
CC viral capsid toward viral factories (By similarity). Seems to induce
CC caspase-3 activation and apoptosis (By similarity). Plays a role in
CC virion morphogenesis by recruiting and transforming the host ER
CC membranes into the precursors of the viral envelope (By similarity).
CC Involved in virus attachment to the host cell (By similarity).
CC {ECO:0000250|UniProtKB:Q65194}.
CC -!- SUBUNIT: Interacts with the host light chain cytoplasmic dynein DYNLL1;
CC this interaction is critical for intracellular microtubule-dependent
CC virus transport toward viral factories. {ECO:0000250|UniProtKB:Q65194}.
CC -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000250|UniProtKB:Q65194};
CC Single-pass membrane protein {ECO:0000250|UniProtKB:Q65194}. Host
CC cytoplasm, host cytoskeleton {ECO:0000250|UniProtKB:Q65194}. Host
CC endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q65194}.
CC Note=Detected mainly on membrane-like structures within viral
CC factories. Present in mature extracellular virions. Host DYNLL1 and
CC viral p54 interact at the microtubular organizing center (By
CC similarity). Found in the inner envelope of the virus (By similarity).
CC {ECO:0000250|UniProtKB:Q65194}.
CC -!- INDUCTION: Expressed in the late phase of the viral replicative cycle.
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the asfivirus envelope protein p54 family.
CC {ECO:0000305}.
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DR EMBL; X71982; CAA50833.1; -; Genomic_DNA.
DR EMBL; X84905; CAA59329.1; -; Genomic_DNA.
DR EMBL; FJ174425; ACJ39044.1; -; Genomic_DNA.
DR EMBL; EU874348; ACJ61607.1; -; Genomic_DNA.
DR EMBL; EU874349; ACJ61608.1; -; Genomic_DNA.
DR EMBL; EU874350; ACJ61609.1; -; Genomic_DNA.
DR EMBL; EU874352; ACJ61611.1; -; Genomic_DNA.
DR EMBL; EU874385; ACJ61644.1; -; Genomic_DNA.
DR EMBL; AY261361; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; S52914; S52914.
DR Proteomes; UP000000860; Genome.
DR GO; GO:0044167; C:host cell endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0044163; C:host cytoskeleton; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0019031; C:viral envelope; IEA:UniProtKB-KW.
DR GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0075521; P:microtubule-dependent intracellular transport of viral material towards nucleus; IEA:UniProtKB-KW.
DR GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR InterPro; IPR008385; ASFV_p54.
DR Pfam; PF05568; ASFV_J13L; 1.
PE 3: Inferred from homology;
KW Apoptosis; Cytoplasmic inwards viral transport; Host cytoplasm;
KW Host cytoskeleton; Host endoplasmic reticulum; Host membrane;
KW Host-virus interaction; Membrane; Microtubular inwards viral transport;
KW Transmembrane; Transmembrane helix; Viral envelope protein; Virion;
KW Virus entry into host cell.
FT CHAIN 1..176
FT /note="Inner membrane protein p54"
FT /id="PRO_0000373418"
FT TRANSMEM 32..52
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 82..176
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 142..154
FT /note="Interaction with host DYNLL1"
FT /evidence="ECO:0000250|UniProtKB:Q65194"
FT COMPBIAS 140..162
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 176 AA; 19021 MW; FC2D5763A2A90296 CRC64;
MDSEFFQPVY PRHYGECLSS TPAPSFFSTH MYTILIAIVV LIIIIIVLIY LFSSRKKKAA
AAIEEEDIQF INPYQDQQWA GVTPQPGIAK PAGASTGSAG KPVMGRPVTN KPVTNKPVTD
RLGMAAGGPA AASAPAHPAE LYTTATTQNT ASQTMPADEN LRQRNTYTHK DLENSL
