ID   P54_ASFWA               Reviewed;         193 AA.
AC   P0CA00;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   05-MAY-2009, sequence version 1.
DT   03-AUG-2022, entry version 45.
DE   RecName: Full=Inner membrane protein p54 {ECO:0000250|UniProtKB:Q65194};
DE   AltName: Full=pE183L {ECO:0000250|UniProtKB:Q65194};
GN   OrderedLocusNames=War-136;
OS   African swine fever virus (isolate Warthog/Namibia/Wart80/1980) (ASFV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Asfuvirales; Asfarviridae; Asfivirus.
OX   NCBI_TaxID=561444;
OH   NCBI_TaxID=6937; Ornithodoros (relapsing fever ticks).
OH   NCBI_TaxID=85517; Phacochoerus aethiopicus (Warthog).
OH   NCBI_TaxID=41426; Phacochoerus africanus (Warthog).
OH   NCBI_TaxID=273792; Potamochoerus larvatus (Bushpig).
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Kutish G.F., Rock D.L.;
RT   "African swine fever virus genomes.";
RL   Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Inner envelope protein involved, through its interaction with
CC       host dynein, in the intracellular microtubule-dependent transport of
CC       viral capsid toward viral factories (By similarity). Seems to induce
CC       caspase-3 activation and apoptosis (By similarity). Plays a role in
CC       virion morphogenesis by recruiting and transforming the host ER
CC       membranes into the precursors of the viral envelope (By similarity).
CC       Involved in virus attachment to the host cell (By similarity).
CC       {ECO:0000250|UniProtKB:Q65194}.
CC   -!- SUBUNIT: Interacts with the host light chain cytoplasmic dynein DYNLL1;
CC       this interaction is critical for intracellular microtubule-dependent
CC       virus transport toward viral factories. {ECO:0000250|UniProtKB:Q65194}.
CC   -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000250|UniProtKB:Q65194};
CC       Single-pass membrane protein {ECO:0000250|UniProtKB:Q65194}. Host
CC       cytoplasm, host cytoskeleton {ECO:0000250|UniProtKB:Q65194}. Host
CC       endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q65194}.
CC       Note=Detected mainly on membrane-like structures within viral
CC       factories. Present in mature extracellular virions. Host DYNLL1 and
CC       viral p54 interact at the microtubular organizing center (By
CC       similarity). Found in the inner envelope of the virus (By similarity).
CC       {ECO:0000250|UniProtKB:Q65194}.
CC   -!- INDUCTION: Expressed in the late phase of the viral replicative cycle.
CC       {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the asfivirus envelope protein p54 family.
CC       {ECO:0000305}.
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DR   EMBL; AY261366; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   Proteomes; UP000000858; Genome.
DR   GO; GO:0044167; C:host cell endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0044163; C:host cytoskeleton; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0019031; C:viral envelope; IEA:UniProtKB-KW.
DR   GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0075521; P:microtubule-dependent intracellular transport of viral material towards nucleus; IEA:UniProtKB-KW.
DR   GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR   InterPro; IPR008385; ASFV_p54.
DR   Pfam; PF05568; ASFV_J13L; 1.
PE   3: Inferred from homology;
KW   Apoptosis; Cytoplasmic inwards viral transport; Host cytoplasm;
KW   Host cytoskeleton; Host endoplasmic reticulum; Host membrane;
KW   Host-virus interaction; Late protein; Membrane;
KW   Microtubular inwards viral transport; Repeat; Transmembrane;
KW   Transmembrane helix; Viral envelope protein; Virion;
KW   Virus entry into host cell.
FT   CHAIN           1..193
FT                   /note="Inner membrane protein p54"
FT                   /id="PRO_0000373420"
FT   TRANSMEM        32..52
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REPEAT          135..138
FT                   /note="1"
FT   REPEAT          139..142
FT                   /note="2"
FT   REPEAT          143..146
FT                   /note="3"
FT   REPEAT          147..150
FT                   /note="4"
FT   REGION          84..126
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          135..150
FT                   /note="4 X 4 AA tandem repeats of A-A-A-S"
FT   REGION          159..171
FT                   /note="Interaction with host DYNLL1"
FT                   /evidence="ECO:0000250|UniProtKB:Q65194"
SQ   SEQUENCE   193 AA;  20637 MW;  728EDECC06F8676B CRC64;
     MDSEFFQPVY PRHYGECLSP VSTPSFFSTH MYTILIAIVV LVIIIIVLIY LFSSRKKKAA
     AAIEEEDIQF INPYQDQQWV EVTPQPGTSK PAGATTASVG KPVTGRPATN RPVTDRPATN
     NPVTDRLVMA TGGPAAASAA ASAAASAAAS APAHPAEPYT TVTTQNTASQ TMSAIENLRQ
     RSTYTHKDLE NSL