ID   P5CR2_DICDI             Reviewed;         299 AA.
AC   Q55E34;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 96.
DE   RecName: Full=Pyrroline-5-carboxylate reductase 2;
DE            Short=P5C reductase 2;
DE            Short=P5CR 2;
DE            EC=1.5.1.2;
GN   Name=pycr2; ORFNames=DDB_G0269410;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=L-proline + NADP(+) = 1-pyrroline-5-carboxylate + 2 H(+) +
CC         NADPH; Xref=Rhea:RHEA:14109, ChEBI:CHEBI:15378, ChEBI:CHEBI:15893,
CC         ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:60039; EC=1.5.1.2;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=L-proline + NAD(+) = 1-pyrroline-5-carboxylate + 2 H(+) +
CC         NADH; Xref=Rhea:RHEA:14105, ChEBI:CHEBI:15378, ChEBI:CHEBI:15893,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:60039; EC=1.5.1.2;
CC   -!- PATHWAY: Amino-acid biosynthesis; L-proline biosynthesis; L-proline
CC       from L-glutamate 5-semialdehyde: step 1/1.
CC   -!- SUBUNIT: Homodecamer; composed of 5 homodimers. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the pyrroline-5-carboxylate reductase family.
CC       {ECO:0000305}.
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DR   EMBL; AAFI02000005; EAL72055.1; -; Genomic_DNA.
DR   RefSeq; XP_645947.1; XM_640855.1.
DR   AlphaFoldDB; Q55E34; -.
DR   SMR; Q55E34; -.
DR   BioGRID; 1241894; 1.
DR   STRING; 44689.DDB0232207; -.
DR   PaxDb; Q55E34; -.
DR   EnsemblProtists; EAL72055; EAL72055; DDB_G0269410.
DR   GeneID; 8616891; -.
DR   KEGG; ddi:DDB_G0269410; -.
DR   dictyBase; DDB_G0269410; -.
DR   eggNOG; KOG3124; Eukaryota.
DR   HOGENOM; CLU_042344_1_2_1; -.
DR   InParanoid; Q55E34; -.
DR   OMA; VVRVMTN; -.
DR   PhylomeDB; Q55E34; -.
DR   UniPathway; UPA00098; UER00361.
DR   PRO; PR:Q55E34; -.
DR   Proteomes; UP000002195; Chromosome 1.
DR   GO; GO:0005739; C:mitochondrion; ISS:UniProtKB.
DR   GO; GO:0004735; F:pyrroline-5-carboxylate reductase activity; IBA:GO_Central.
DR   GO; GO:0055129; P:L-proline biosynthetic process; IBA:GO_Central.
DR   GO; GO:0006561; P:proline biosynthetic process; ISS:UniProtKB.
DR   HAMAP; MF_01925; P5C_reductase; 1.
DR   InterPro; IPR008927; 6-PGluconate_DH-like_C_sf.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR028939; P5C_Rdtase_cat_N.
DR   InterPro; IPR029036; P5CR_dimer.
DR   InterPro; IPR000304; Pyrroline-COOH_reductase.
DR   PANTHER; PTHR11645; PTHR11645; 1.
DR   Pfam; PF03807; F420_oxidored; 1.
DR   Pfam; PF14748; P5CR_dimer; 1.
DR   PIRSF; PIRSF000193; Pyrrol-5-carb_rd; 1.
DR   SUPFAM; SSF48179; SSF48179; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
PE   3: Inferred from homology;
KW   Amino-acid biosynthesis; NADP; Oxidoreductase; Proline biosynthesis;
KW   Reference proteome.
FT   CHAIN           1..299
FT                   /note="Pyrroline-5-carboxylate reductase 2"
FT                   /id="PRO_0000328305"
SQ   SEQUENCE   299 AA;  31719 MW;  FFE0C3E5B14122DD CRC64;
     MVATDLKNNK NIAILGSGNL GVSIARGIVA SGHYKSNQIV LTRRTLEKIQ HLKDEEGFQI
     TSDNLEAANK CAILIIGVLP AQVPQLLESI KHLVTKDHII ISVVLGITIS GIRSHLQPDV
     YTPIVRAMPN TAIQYCESMT CIANKSDHPT KSGTAEQELA DQNALEVTEK IFNCCGECIT
     ISEEAMVSAP ALCSCGTAFF CRIIRAAASA GCEIGFHAED AVRIAAQTAK GAAIMLLKND
     SHPESEIDRI TTPSGATIAG LNTMEHNGLS SAIIKGIVMS AEKSSKSQAA LNKLNNVSS