ID   P5CR_PEA                Reviewed;         273 AA.
AC   Q04708;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1993, sequence version 1.
DT   03-AUG-2022, entry version 97.
DE   RecName: Full=Pyrroline-5-carboxylate reductase;
DE            Short=P5C reductase;
DE            Short=P5CR;
DE            EC=1.5.1.2;
GN   Name=PROC;
OS   Pisum sativum (Garden pea).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; Hologalegina; IRL clade; Fabeae; Pisum.
OX   NCBI_TaxID=3888;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=11537868; DOI=10.1104/pp.100.3.1464;
RA   Williamson C.L., Slocum R.D.;
RT   "Molecular cloning and evidence for osmoregulation of the delta 1-
RT   pyrroline-5-carboxylate reductase (proC) gene in pea (Pisum sativum L.).";
RL   Plant Physiol. 100:1464-1470(1992).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=L-proline + NADP(+) = 1-pyrroline-5-carboxylate + 2 H(+) +
CC         NADPH; Xref=Rhea:RHEA:14109, ChEBI:CHEBI:15378, ChEBI:CHEBI:15893,
CC         ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:60039; EC=1.5.1.2;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=L-proline + NAD(+) = 1-pyrroline-5-carboxylate + 2 H(+) +
CC         NADH; Xref=Rhea:RHEA:14105, ChEBI:CHEBI:15378, ChEBI:CHEBI:15893,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:60039; EC=1.5.1.2;
CC   -!- PATHWAY: Amino-acid biosynthesis; L-proline biosynthesis; L-proline
CC       from L-glutamate 5-semialdehyde: step 1/1.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the pyrroline-5-carboxylate reductase family.
CC       {ECO:0000305}.
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DR   EMBL; X62842; CAA44646.1; -; mRNA.
DR   PIR; T06477; T06477.
DR   AlphaFoldDB; Q04708; -.
DR   SMR; Q04708; -.
DR   PRIDE; Q04708; -.
DR   EnsemblPlants; Psat3g078000.1; Psat3g078000.1.cds; Psat3g078000.
DR   Gramene; Psat3g078000.1; Psat3g078000.1.cds; Psat3g078000.
DR   SABIO-RK; Q04708; -.
DR   UniPathway; UPA00098; UER00361.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004735; F:pyrroline-5-carboxylate reductase activity; IEA:UniProtKB-EC.
DR   GO; GO:0055129; P:L-proline biosynthetic process; IEA:UniProtKB-UniPathway.
DR   HAMAP; MF_01925; P5C_reductase; 1.
DR   InterPro; IPR008927; 6-PGluconate_DH-like_C_sf.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR028939; P5C_Rdtase_cat_N.
DR   InterPro; IPR029036; P5CR_dimer.
DR   InterPro; IPR000304; Pyrroline-COOH_reductase.
DR   PANTHER; PTHR11645; PTHR11645; 1.
DR   Pfam; PF03807; F420_oxidored; 1.
DR   Pfam; PF14748; P5CR_dimer; 1.
DR   PIRSF; PIRSF000193; Pyrrol-5-carb_rd; 1.
DR   SUPFAM; SSF48179; SSF48179; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   TIGRFAMs; TIGR00112; proC; 1.
DR   PROSITE; PS00521; P5CR; 1.
PE   2: Evidence at transcript level;
KW   Amino-acid biosynthesis; Cytoplasm; NADP; Oxidoreductase;
KW   Proline biosynthesis.
FT   CHAIN           1..273
FT                   /note="Pyrroline-5-carboxylate reductase"
FT                   /id="PRO_0000187324"
SQ   SEQUENCE   273 AA;  28238 MW;  D719F52935966588 CRC64;
     MEILPILSDS YTLGFIGAGK MAESIAKGAS RSGVLPSSRI VTAHSNPSRR AAFESIGITV
     LSSNDDVVRA SNVVVFSVKP QLVKDVVLKL KPLLTKDKLL VSVAAGIKLK DLQEWAGHER
     FIRVMPNTPA AVGQAASVMS LGGAATEEDA NLISQLFGSI GKIWKADDKF FDAITGLSGS
     GPAYIYLAIE ALADGGVAAG LPRDLALSLA SQTVLGAASM ATLSGKHPGQ LKDDVTSPGG
     TTIAGVHELE KGGFRGTLMN AVVAAAKRSR ELS