P5CR_SCHPO
ID P5CR_SCHPO Reviewed; 282 AA.
AC Q9P7Y7;
DT 27-MAR-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 25-MAY-2022, entry version 130.
DE RecName: Full=Pyrroline-5-carboxylate reductase;
DE Short=P5C reductase;
DE Short=P5CR;
DE EC=1.5.1.2;
GN Name=pro3; ORFNames=SPAPYUG7.05;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-96.
RC STRAIN=972 / ATCC 24843;
RX PubMed=11041002;
RA Shpakovski G.V., Baranova G.M.;
RT "Chromosomal localization of the rpb9+ and tfa1+ genes encoding components
RT of the mRNA synthesis machinery of Schizosaccharomyces pombe.";
RL Bioorg. Khim. 26:623-630(2000).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-proline + NADP(+) = 1-pyrroline-5-carboxylate + 2 H(+) +
CC NADPH; Xref=Rhea:RHEA:14109, ChEBI:CHEBI:15378, ChEBI:CHEBI:15893,
CC ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:60039; EC=1.5.1.2;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-proline + NAD(+) = 1-pyrroline-5-carboxylate + 2 H(+) +
CC NADH; Xref=Rhea:RHEA:14105, ChEBI:CHEBI:15378, ChEBI:CHEBI:15893,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:60039; EC=1.5.1.2;
CC -!- PATHWAY: Amino-acid biosynthesis; L-proline biosynthesis; L-proline
CC from L-glutamate 5-semialdehyde: step 1/1.
CC -!- SIMILARITY: Belongs to the pyrroline-5-carboxylate reductase family.
CC {ECO:0000305}.
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DR EMBL; CU329670; CAB66314.1; -; Genomic_DNA.
DR EMBL; AF237418; AAL55659.1; -; Genomic_DNA.
DR PIR; T50305; T50305.
DR RefSeq; NP_594706.1; NM_001020133.2.
DR AlphaFoldDB; Q9P7Y7; -.
DR SMR; Q9P7Y7; -.
DR STRING; 4896.SPAPYUG7.05.1; -.
DR MaxQB; Q9P7Y7; -.
DR PaxDb; Q9P7Y7; -.
DR EnsemblFungi; SPAPYUG7.05.1; SPAPYUG7.05.1:pep; SPAPYUG7.05.
DR GeneID; 2543212; -.
DR KEGG; spo:SPAPYUG7.05; -.
DR PomBase; SPAPYUG7.05; -.
DR VEuPathDB; FungiDB:SPAPYUG7.05; -.
DR eggNOG; KOG3124; Eukaryota.
DR HOGENOM; CLU_042344_1_1_1; -.
DR InParanoid; Q9P7Y7; -.
DR OMA; VVRVMTN; -.
DR PhylomeDB; Q9P7Y7; -.
DR Reactome; R-SPO-8964539; Glutamate and glutamine metabolism.
DR UniPathway; UPA00098; UER00361.
DR PRO; PR:Q9P7Y7; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0004735; F:pyrroline-5-carboxylate reductase activity; ISO:PomBase.
DR GO; GO:0055129; P:L-proline biosynthetic process; IBA:GO_Central.
DR HAMAP; MF_01925; P5C_reductase; 1.
DR InterPro; IPR008927; 6-PGluconate_DH-like_C_sf.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR028939; P5C_Rdtase_cat_N.
DR InterPro; IPR029036; P5CR_dimer.
DR InterPro; IPR000304; Pyrroline-COOH_reductase.
DR PANTHER; PTHR11645; PTHR11645; 1.
DR Pfam; PF03807; F420_oxidored; 1.
DR Pfam; PF14748; P5CR_dimer; 1.
DR PIRSF; PIRSF000193; Pyrrol-5-carb_rd; 1.
DR SUPFAM; SSF48179; SSF48179; 1.
DR SUPFAM; SSF51735; SSF51735; 1.
DR TIGRFAMs; TIGR00112; proC; 1.
DR PROSITE; PS00521; P5CR; 1.
PE 3: Inferred from homology;
KW Amino-acid biosynthesis; NADP; Oxidoreductase; Proline biosynthesis;
KW Reference proteome.
FT CHAIN 1..282
FT /note="Pyrroline-5-carboxylate reductase"
FT /id="PRO_0000187327"
SQ SEQUENCE 282 AA; 29875 MW; 8874A4CB98690CF7 CRC64;
MSGFCVLGCG TMGKALLTGI FDSIAENGND VSDEIIIPNK FYACVKFPKE KEDVQKLFGD
RVKVVMGAKE NAEMAAISNV LLLSCKPQAA EDVLNSPKMK EALKGKLILS ILAGKTISSL
QSMLDESTRV IRIMPNTASR IRESMSVICP GPNATEEDIK FAEWVFNGIG RSMKLPEKLI
DAATAVCGSG PAFVATMIEA MTDGGVMMGI PFPQAQELAA QTMVGTGRMV LQGQHPAMIR
NDVSTPAGCT ISGLLALEDG KIRSTIARGI EQATKTASGL GK
