P5F12_XENLA
ID P5F12_XENLA Reviewed; 445 AA.
AC B7ZQA9; Q03917; Q9PSI7;
DT 15-DEC-2009, integrated into UniProtKB/Swiss-Prot.
DT 03-MAR-2009, sequence version 1.
DT 03-AUG-2022, entry version 69.
DE RecName: Full=POU domain, class 5, transcription factor 1.2;
DE AltName: Full=POU class V protein oct-91 {ECO:0000303|PubMed:16860542};
DE Short=XlPOU91;
DE Short=Xoct-91 {ECO:0000312|EMBL:AAI69741.1};
GN Name=pou5f1.2; Synonyms=oct-91 {ECO:0000312|EMBL:AAI69741.1};
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1] {ECO:0000305, ECO:0000312|EMBL:AAA49999.1}
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RC TISSUE=Gastrula {ECO:0000269|PubMed:1732736};
RX PubMed=1732736; DOI=10.1128/mcb.12.2.638-649.1992;
RA Hinkley C.S., Martin J.F., Leibham D., Perry M.;
RT "Sequential expression of multiple POU proteins during amphibian early
RT development.";
RL Mol. Cell. Biol. 12:638-649(1992).
RN [2] {ECO:0000312|EMBL:AAI69741.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Gastrula {ECO:0000312|EMBL:AAI69741.1};
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (NOV-2008) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [MRNA] OF 236-362, TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RC TISSUE=Gastrula {ECO:0000269|PubMed:1358592};
RX PubMed=1358592; DOI=10.1242/dev.115.2.439;
RA Frank D., Harland R.M.;
RT "Localized expression of a Xenopus POU gene depends on cell-autonomous
RT transcriptional activation and induction-dependent inactivation.";
RL Development 115:439-448(1992).
RN [4] {ECO:0000305}
RP FUNCTION.
RX PubMed=16860542; DOI=10.1016/j.mod.2006.06.004;
RA Cao Y., Siegel D., Knochel W.;
RT "Xenopus POU factors of subclass V inhibit activin/nodal signaling during
RT gastrulation.";
RL Mech. Dev. 123:614-625(2006).
RN [5] {ECO:0000305}
RP INTERACTION WITH TCF7L1 AND VEGT.
RX PubMed=17541407; DOI=10.1038/sj.emboj.7601736;
RA Cao Y., Siegel D., Donow C., Knochel S., Yuan L., Knochel W.;
RT "POU-V factors antagonize maternal VegT activity and beta-Catenin signaling
RT in Xenopus embryos.";
RL EMBO J. 26:2942-2954(2007).
CC -!- FUNCTION: Transcription factor that binds to the octamer motif (5'-
CC ATTTGCAT-3'). Antagonizes the activity of nodal/activin signaling
CC during gastrulation to suppress mesendoderm formation.
CC {ECO:0000269|PubMed:16860542, ECO:0000269|PubMed:1732736}.
CC -!- SUBUNIT: Interacts with the transcription factors tcf7l1/tcf3 and vegt.
CC {ECO:0000269|PubMed:17541407}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q01860,
CC ECO:0000255|PROSITE-ProRule:PRU00108, ECO:0000255|PROSITE-
CC ProRule:PRU00530}.
CC -!- TISSUE SPECIFICITY: Initially (stage 9) expressed in all regions of the
CC embryo, becoming localized to the ventroposterior regions by early
CC neurula stages. In adults, expressed at a low level in the brain.
CC {ECO:0000269|PubMed:1358592, ECO:0000269|PubMed:1732736}.
CC -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically.
CC Expressed at low levels in oocytes, with expression increasing rapidly
CC during gastrulation and peaking during late gastrulation before
CC declining through neurulation. {ECO:0000269|PubMed:1358592,
CC ECO:0000269|PubMed:1732736}.
CC -!- SIMILARITY: Belongs to the POU transcription factor family. Class-5
CC subfamily. {ECO:0000255}.
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DR EMBL; M60077; AAA49999.1; -; mRNA.
DR EMBL; BC169741; AAI69741.1; -; mRNA.
DR EMBL; BC169743; AAI69743.1; -; mRNA.
DR PIR; C42022; C42022.
DR RefSeq; NP_001081342.1; NM_001087873.1.
DR AlphaFoldDB; B7ZQA9; -.
DR SMR; B7ZQA9; -.
DR IntAct; B7ZQA9; 2.
DR MINT; B7ZQA9; -.
DR GeneID; 397784; -.
DR KEGG; xla:397784; -.
DR CTD; 397784; -.
DR Xenbase; XB-GENE-6252587; pou5f3.1.L.
DR OMA; YPYIREN; -.
DR OrthoDB; 668779at2759; -.
DR Proteomes; UP000186698; Chromosome 8L.
DR Bgee; 397784; Expressed in gastrula and 11 other tissues.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0005667; C:transcription regulator complex; IPI:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR GO; GO:0140297; F:DNA-binding transcription factor binding; IPI:UniProtKB.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.
DR GO; GO:0007369; P:gastrulation; IMP:UniProtKB.
DR CDD; cd00086; homeodomain; 1.
DR Gene3D; 1.10.260.40; -; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR InterPro; IPR013847; POU.
DR InterPro; IPR000327; POU_dom.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF00157; Pou; 1.
DR PRINTS; PR00028; POUDOMAIN.
DR SMART; SM00389; HOX; 1.
DR SMART; SM00352; POU; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR SUPFAM; SSF47413; SSF47413; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
DR PROSITE; PS00465; POU_2; 1.
DR PROSITE; PS51179; POU_3; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Homeobox; Nucleus; Reference proteome.
FT CHAIN 1..445
FT /note="POU domain, class 5, transcription factor 1.2"
FT /id="PRO_0000390497"
FT DOMAIN 218..292
FT /note="POU-specific"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00530"
FT DNA_BIND 312..371
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 76..116
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 139..227
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 90..107
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 165..219
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 248
FT /note="G -> A (in Ref. 3)"
FT /evidence="ECO:0000305"
FT CONFLICT 295
FT /note="E -> K (in Ref. 1; AAA49999)"
FT /evidence="ECO:0000305"
FT CONFLICT 333
FT /note="Q -> G (in Ref. 3)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 445 AA; 49036 MW; C981C1F3A2EDA93C CRC64;
MYNQQTYPSF THNPALMPDG SGQYNLGTYT GMARHPHQAQ AFFPFSGVKS DYGDLGGQTT
SVGDTSAWNP LTSLDSANQL GISGQGNPFK NLKREREDDE EKSESPEPKC SPPSLPPAYY
THAWNPTTTF WSQVSSSGTT VVSKPLPTPL QPGDKCDPVE ANKIFTSSPD KSGESGISSL
DNSRCSSATS SSSGGTNVGT PRSLSRGASD GLSSDSEEEA PNSGEMEQFA KDLKHKRITM
GYTQADVGYA LGVLFGKTFS QTTICRFESL QLSFKNMCKL KPLLRSWLHE VENNENLQEI
ISRGQIIPQV QKRKHRTSIE NNVKCTLENY FMQCSKPSAQ EIAQIARELN MEKDVVRVWF
CNRRQKGKRQ VYPYIRENGG EPYDAPQTLT PPSQGPFPLP QVMPSQVFPT VPLGANPTIY
VPTYHKNDMF PQAMHHGIGM GNQGN
