ID   P5F13_XENTR             Reviewed;         423 AA.
AC   B3DM23;
DT   19-JAN-2010, integrated into UniProtKB/Swiss-Prot.
DT   22-JUL-2008, sequence version 1.
DT   03-AUG-2022, entry version 75.
DE   RecName: Full=POU domain, class 5, transcription factor 1.3;
DE   AltName: Full=POU class V protein oct-60 {ECO:0000250|UniProtKB:Q91989};
GN   Name=pou5f1.3; Synonyms=oct-60 {ECO:0000250|UniProtKB:Q91989};
OS   Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX   NCBI_TaxID=8364;
RN   [1] {ECO:0000312|EMBL:AAI67672.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Gastrula {ECO:0000312|EMBL:AAI67672.1};
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Transcription factor that binds to the octamer motif (5'-
CC       ATTTGCAT-3'). Antagonizes the activity of nodal/activin signaling
CC       during gastrulation to suppress mesendoderm formation. Acts maternally
CC       to inhibit vegt and beta-catenin-activated gene transcription, probably
CC       by forming a transcriptional repression complex on the promoters of
CC       target genes. Binds to an octamer motif in interspersed RNA (By
CC       similarity). {ECO:0000250|UniProtKB:Q91989}.
CC   -!- SUBUNIT: Interacts with the transcription factors tcf7l1/tcf3 and vegt.
CC       {ECO:0000250|UniProtKB:Q91989}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q01860,
CC       ECO:0000255|PROSITE-ProRule:PRU00108, ECO:0000255|PROSITE-
CC       ProRule:PRU00530}.
CC   -!- SIMILARITY: Belongs to the POU transcription factor family. Class-5
CC       subfamily. {ECO:0000255}.
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DR   EMBL; BC167672; AAI67672.1; -; mRNA.
DR   RefSeq; NP_001123836.1; NM_001130364.1.
DR   AlphaFoldDB; B3DM23; -.
DR   SMR; B3DM23; -.
DR   STRING; 8364.ENSXETP00000059598; -.
DR   PaxDb; B3DM23; -.
DR   PRIDE; B3DM23; -.
DR   GeneID; 100170593; -.
DR   KEGG; xtr:100170593; -.
DR   CTD; 100170593; -.
DR   Xenbase; XB-GENE-5903504; pou5f3.3.
DR   eggNOG; KOG3802; Eukaryota.
DR   InParanoid; B3DM23; -.
DR   OrthoDB; 668779at2759; -.
DR   Proteomes; UP000008143; Chromosome 8.
DR   Proteomes; UP000790000; Unplaced.
DR   Bgee; ENSXETG00000026475; Expressed in gastrula and 7 other tissues.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005667; C:transcription regulator complex; ISS:UniProtKB.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0140297; F:DNA-binding transcription factor binding; ISS:UniProtKB.
DR   GO; GO:0003723; F:RNA binding; ISS:UniProtKB.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0043565; F:sequence-specific DNA binding; ISS:UniProtKB.
DR   GO; GO:0000976; F:transcription cis-regulatory region binding; ISS:UniProtKB.
DR   GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR   GO; GO:0032926; P:negative regulation of activin receptor signaling pathway; ISS:UniProtKB.
DR   GO; GO:0045892; P:negative regulation of transcription, DNA-templated; ISS:UniProtKB.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   CDD; cd00086; homeodomain; 1.
DR   CDD; cd00093; HTH_XRE; 1.
DR   Gene3D; 1.10.260.40; -; 1.
DR   InterPro; IPR001387; Cro/C1-type_HTH.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR017970; Homeobox_CS.
DR   InterPro; IPR001356; Homeobox_dom.
DR   InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR   InterPro; IPR013847; POU.
DR   InterPro; IPR000327; POU_dom.
DR   Pfam; PF00046; Homeodomain; 1.
DR   Pfam; PF00157; Pou; 1.
DR   PRINTS; PR00028; POUDOMAIN.
DR   SMART; SM00389; HOX; 1.
DR   SMART; SM00352; POU; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   SUPFAM; SSF47413; SSF47413; 1.
DR   PROSITE; PS00027; HOMEOBOX_1; 1.
DR   PROSITE; PS50071; HOMEOBOX_2; 1.
DR   PROSITE; PS00465; POU_2; 1.
DR   PROSITE; PS51179; POU_3; 1.
PE   2: Evidence at transcript level;
KW   Developmental protein; Differentiation; DNA-binding; Homeobox; Nucleus;
KW   Reference proteome; RNA-binding; Transcription; Transcription regulation.
FT   CHAIN           1..423
FT                   /note="POU domain, class 5, transcription factor 1.3"
FT                   /id="PRO_0000390935"
FT   DOMAIN          207..281
FT                   /note="POU-specific"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00530"
FT   DNA_BIND        301..360
FT                   /note="Homeobox"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT   REGION          85..211
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        108..122
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        157..207
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   423 AA;  46732 MW;  62D85655A840D8BD CRC64;
     MDQPVLYNQP AFPNFSYSPG LGQEGGNYQY LGNYNAPSYP QPFFHVPPAI KSEYGAQDEV
     TGGSCHAAPF DWHLYPHFQF SNQVGALSSG DPSPEGRNEE DHGSISEERS SGTPSPNSPM
     VPSYAQYWHH APWQGNPTGQ ALGLPSRAHP DGGEKPQQSD CSPTASLESG ASNTEDEEVS
     SALSSRAERG LCSPSPNNAS FGSGNEEDGT TLEEMEEFAK ELKQKRVALG YTQGDIGHAL
     GILYGKMFSQ TTICRFESLQ LTFKNMCKLK PLLEQWLGEA ENNDNLQEMI HKAQLEEQNR
     KRKMRTCFDS VLKGRLEGHF MCNQKPGARE LAEIAKELGL EKDVVRVWFC NRRQKEKSKS
     RMSKAHEFVG GASPVPSPAE HISQDYGLAP LHPNRPPFYP PPFPRNDLFP HMVPGMSMGV
     LTG