ASI1A_EUTRU
ID ASI1A_EUTRU Reviewed; 51 AA.
AC P0DQJ1;
DT 16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT 16-OCT-2019, sequence version 1.
DT 25-MAY-2022, entry version 6.
DE RecName: Full=U-Asilidin(1)-Eru1a {ECO:0000303|PubMed:29303983};
DE Flags: Precursor;
OS Eutolmus rufibarbis (Golden-tabbed robberfly) (Asilus rufibarbis).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Asiloidea;
OC Asilidae; Asilinae; Eutolmus.
OX NCBI_TaxID=1936067;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], IDENTIFICATION BY MASS SPECTROMETRY, AND
RP SUBCELLULAR LOCATION.
RC TISSUE=Venom, and Venom gland;
RX PubMed=29303983; DOI=10.3390/toxins10010029;
RA Drukewitz S.H., Fuhrmann N., Undheim E.A.B., Blanke A., Giribaldi J.,
RA Mary R., Laconde G., Dutertre S., von Reumont B.M.;
RT "A Dipteran's novel sucker punch: evolution of arthropod atypical venom
RT with a neurotoxic component in robber fliezs (Asilidae, Diptera).";
RL Toxins 10:0-0(2018).
CC -!- FUNCTION: Induces neurotoxic effect on honeybees, including slow
CC movements, disorientation and paralysis. Since it provokes similar
CC symptoms than omega-atracotoxin, it is probable that it acts in the
CC same way by inhibiting voltage-gated calcium channels.
CC {ECO:0000250|UniProtKB:P0DQI8}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:29303983}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland. The most highly
CC expressed peptides U-Asilidin1-Mar1a is around 3000 times higher
CC expressed in the venom thoracic glands compared to its body tissues.
CC {ECO:0000305|PubMed:29303983}.
CC -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC structurally defines this protein as a knottin.
CC {ECO:0000305|PubMed:29303983}.
CC -!- SIMILARITY: Belongs to the asilidin-1 family. {ECO:0000305}.
CC -!- WEB RESOURCE: Name=National Center for Biotechnology Information
CC (NCBI);
CC URL="https://www.ncbi.nlm.nih.gov/nuccore/GFZQ00000000";
CC -!- WEB RESOURCE: Name=National Center for Biotechnology Information
CC (NCBI);
CC URL="https://www.ncbi.nlm.nih.gov/nuccore/GFFZ00000000";
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DR AlphaFoldDB; P0DQJ1; -.
DR SMR; P0DQJ1; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW Disulfide bond; Knottin; Neurotoxin; Secreted; Signal; Toxin.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT PEPTIDE 24..51
FT /note="U-Asilidin(1)-Eru1a"
FT /id="PRO_0000448213"
FT DISULFID 26..40
FT /evidence="ECO:0000305|PubMed:29303983"
FT DISULFID 33..44
FT /evidence="ECO:0000305|PubMed:29303983"
FT DISULFID 39..49
FT /evidence="ECO:0000305|PubMed:29303983"
SQ SEQUENCE 51 AA; 5498 MW; FB685B3815B5C21A CRC64;
MANYIDVLSF LAIICATVLA TLAQDCSPEG ARCVHDSECC FNECIDSLCQ P