ID   P65_MYCPB               Reviewed;         407 AA.
AC   E1QC64; P53663; P53664; Q6LAC2; Q6LAC3;
DT   08-FEB-2011, integrated into UniProtKB/Swiss-Prot.
DT   30-NOV-2010, sequence version 1.
DT   03-AUG-2022, entry version 43.
DE   RecName: Full=Proline-rich P65 protein;
GN   Name=p65; OrderedLocusNames=MPNE_0359;
OS   Mycoplasma pneumoniae (strain ATCC 15531 / DSM 23978 / CIP 103766 / NBRC
OS   14401 / NCTC 10119 / FH) (Mycoplasmoides pneumoniae).
OC   Bacteria; Tenericutes; Mollicutes; Mycoplasmataceae; Mycoplasma.
OX   NCBI_TaxID=722438;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND SUBCELLULAR LOCATION.
RC   STRAIN=ATCC 15531 / DSM 23978 / CIP 103766 / NBRC 14401 / NCTC 10119 / FH;
RX   PubMed=7768845; DOI=10.1128/jb.177.12.3370-3378.1995;
RA   Proft T., Hilbert H., Layh-Schmitt G., Herrmann R.;
RT   "The proline-rich P65 protein of Mycoplasma pneumoniae is a component of
RT   the Triton X-100-insoluble fraction and exhibits size polymorphism in the
RT   strains M129 and FH.";
RL   J. Bacteriol. 177:3370-3378(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15531 / DSM 23978 / CIP 103766 / NBRC 14401 / NCTC 10119 / FH;
RX   PubMed=20543037; DOI=10.1128/aem.00024-10;
RA   Krishnakumar R., Assad-Garcia N., Benders G.A., Phan Q., Montague M.G.,
RA   Glass J.I.;
RT   "Targeted chromosomal knockouts in Mycoplasma pneumoniae.";
RL   Appl. Environ. Microbiol. 76:5297-5299(2010).
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:7768845};
CC       Peripheral membrane protein {ECO:0000269|PubMed:7768845}. Note=Probably
CC       with epitopes exposed at the cell surface.
CC   -!- DOMAIN: The penta/hexapeptides repeats form a proline-rich acidic
CC       domain. In addition, a part of this region contains a perfect direct
CC       repeat.
CC   -!- PTM: The N-terminus is blocked. {ECO:0000305}.
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DR   EMBL; Z34978; CAA84430.1; -; Genomic_DNA.
DR   EMBL; CP002077; ADK87002.1; -; Genomic_DNA.
DR   RefSeq; WP_014574942.1; NC_017504.1.
DR   AlphaFoldDB; E1QC64; -.
DR   STRING; 722438.MPNE_0359; -.
DR   PaxDb; E1QC64; -.
DR   EnsemblBacteria; ADK87002; ADK87002; MPNE_0359.
DR   KEGG; mpj:MPNE_0359; -.
DR   PATRIC; fig|722438.3.peg.344; -.
DR   eggNOG; COG3266; Bacteria.
DR   HOGENOM; CLU_741505_0_0_14; -.
DR   OMA; DYAQDPN; -.
DR   Proteomes; UP000007756; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR011004; Trimer_LpxA-like_sf.
DR   SUPFAM; SSF51161; SSF51161; 1.
PE   4: Predicted;
KW   Cell membrane; Membrane; Repeat.
FT   CHAIN           1..407
FT                   /note="Proline-rich P65 protein"
FT                   /id="PRO_0000405027"
FT   REPEAT          40..45
FT                   /note="1"
FT   REPEAT          75..80
FT                   /note="2"
FT   REPEAT          83..87
FT                   /note="3"
FT   REPEAT          89..93
FT                   /note="4"
FT   REPEAT          95..99
FT                   /note="5"
FT   REPEAT          101..105
FT                   /note="6"
FT   REPEAT          107..111
FT                   /note="7"
FT   REPEAT          119..123
FT                   /note="8"
FT   REPEAT          140..145
FT                   /note="9"
FT   REPEAT          150..154
FT                   /note="10"
FT   REPEAT          156..160
FT                   /note="11"
FT   REPEAT          170..174
FT                   /note="12"
FT   REGION          1..50
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          40..174
FT                   /note="12 X 5 AA repeats of D-P-N-Q-A-Y"
FT   COMPBIAS        1..30
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        5
FT                   /note="K -> E (in Ref. 1; CAA84430)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        147
FT                   /note="A -> ADPNAYQDPNAYTDPNA (in Ref. 1; CAA84430)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        171
FT                   /note="H -> P (in Ref. 1; CAA84430)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   407 AA;  47342 MW;  D6B09E6F77582D26 CRC64;
     MDINKPGWNQ SDQQATAYDP NQQQYYGDGS TYYDPDQAVD PNQAYYPDPN TYPDAAAYYG
     YGQDGQAYPQ DYAQDPNQAY YADPNAYQDP NAYTDPNAYV DPNAYQDPNA YVDPNNYTDP
     NAYYGYGQDG QAYPQDYAQD PNQAYYAYVD PNAYQDPNAY TDPYYVTSTD HNAYYGQVDN
     VPALEASDLA YEVTPQEQAA EQELFSEPET KVIREIHEFP FEKIRSYFQT DFDSYNSRLT
     QLKDKLDNAI FSMRKAIDTV KENSANLQIM KQNFERQLKE QQTQRLTSNT DAEKIGAKIN
     QLEERMQRLS RTMESVEWTK KEPRQEQFDP RFVDPRNFNN YVNNTDTMMS MFEKVLMMNL
     LRSTTPVQPP VQYFTPQPLT ASPRPVYEEP ISASFRRRGY RGDEFYE