ID   P65_MYCPN               Reviewed;         405 AA.
AC   P0CJ81; P53663; P53664; Q6LAC2; Q6LAC3;
DT   08-FEB-2011, integrated into UniProtKB/Swiss-Prot.
DT   08-FEB-2011, sequence version 1.
DT   03-AUG-2022, entry version 30.
DE   RecName: Full=Proline-rich P65 protein;
GN   Name=p65; OrderedLocusNames=MPN_309; ORFNames=MP527;
OS   Mycoplasma pneumoniae (strain ATCC 29342 / M129) (Mycoplasmoides
OS   pneumoniae).
OC   Bacteria; Tenericutes; Mollicutes; Mycoplasmataceae; Mycoplasma.
OX   NCBI_TaxID=272634;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND SUBCELLULAR LOCATION.
RC   STRAIN=ATCC 29342 / M129;
RX   PubMed=7768845; DOI=10.1128/jb.177.12.3370-3378.1995;
RA   Proft T., Hilbert H., Layh-Schmitt G., Herrmann R.;
RT   "The proline-rich P65 protein of Mycoplasma pneumoniae is a component of
RT   the Triton X-100-insoluble fraction and exhibits size polymorphism in the
RT   strains M129 and FH.";
RL   J. Bacteriol. 177:3370-3378(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 29342 / M129;
RX   PubMed=9098066; DOI=10.1128/jb.179.8.2668-2677.1997;
RA   Krause D.C., Proft T., Hedreyda C.T., Hilbert H., Plagens H., Herrmann R.;
RT   "Transposon mutagenesis reinforces the correlation between Mycoplasma
RT   pneumoniae cytoskeletal protein HMW2 and cytadherence.";
RL   J. Bacteriol. 179:2668-2677(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29342 / M129;
RX   PubMed=8948633; DOI=10.1093/nar/24.22.4420;
RA   Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.;
RT   "Complete sequence analysis of the genome of the bacterium Mycoplasma
RT   pneumoniae.";
RL   Nucleic Acids Res. 24:4420-4449(1996).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 40-75 AND 83-95, AND SUBCELLULAR
RP   LOCATION.
RC   STRAIN=ATCC 29342 / M129;
RX   PubMed=7984111; DOI=10.1111/j.1365-2958.1994.tb00427.x;
RA   Proft T., Herrmann R.;
RT   "Identification and characterization of hitherto unknown Mycoplasma
RT   pneumoniae proteins.";
RL   Mol. Microbiol. 13:337-348(1994).
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:7768845,
CC       ECO:0000269|PubMed:7984111}; Peripheral membrane protein
CC       {ECO:0000269|PubMed:7768845, ECO:0000269|PubMed:7984111}. Note=Probably
CC       with epitopes exposed at the cell surface.
CC   -!- DOMAIN: The penta/hexapeptides repeats form a proline-rich acidic
CC       domain. In addition, a part of this region contains a perfect direct
CC       repeat.
CC   -!- PTM: The N-terminus is blocked. {ECO:0000305}.
CC   -!- CAUTION: The protein sequence for 83-95 might be that for residues 148-
CC       160; the 2 sequences are identical. {ECO:0000305|PubMed:7984111}.
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DR   EMBL; Z34977; CAA84429.1; -; Genomic_DNA.
DR   EMBL; U59896; AAB52526.1; -; Genomic_DNA.
DR   EMBL; U00089; AAB96175.1; -; Genomic_DNA.
DR   EMBL; Z32653; CAA83574.1; -; Genomic_DNA.
DR   EMBL; Z32655; CAA83576.1; -; Genomic_DNA.
DR   PIR; S73853; S73853.
DR   RefSeq; NP_109997.1; NC_000912.1.
DR   RefSeq; WP_010874665.1; NC_000912.1.
DR   AlphaFoldDB; P0CJ81; -.
DR   IntAct; P0CJ81; 1.
DR   STRING; 272634.MPN_309; -.
DR   PRIDE; P0CJ81; -.
DR   EnsemblBacteria; AAB96175; AAB96175; MPN_309.
DR   KEGG; mpn:MPN_309; -.
DR   PATRIC; fig|272634.6.peg.333; -.
DR   HOGENOM; CLU_741505_0_0_14; -.
DR   BioCyc; MPNE272634:G1GJ3-491-MON; -.
DR   Proteomes; UP000000808; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
PE   4: Predicted;
KW   Cell membrane; Membrane; Reference proteome; Repeat.
FT   CHAIN           1..405
FT                   /note="Proline-rich P65 protein"
FT                   /id="PRO_0000058145"
FT   REPEAT          40..45
FT                   /note="1"
FT   REPEAT          75..80
FT                   /note="2"
FT   REPEAT          83..87
FT                   /note="3"
FT   REPEAT          89..93
FT                   /note="4"
FT   REPEAT          95..99
FT                   /note="5"
FT   REPEAT          101..105
FT                   /note="6"
FT   REPEAT          107..111
FT                   /note="7"
FT   REPEAT          119..123
FT                   /note="8"
FT   REPEAT          140..145
FT                   /note="9"
FT   REPEAT          148..152
FT                   /note="10"
FT   REPEAT          154..158
FT                   /note="11"
FT   REPEAT          168..172
FT                   /note="12"
FT   REGION          1..58
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          40..172
FT                   /note="12 X 5 AA repeats of D-P-N-Q-A-Y"
FT   COMPBIAS        1..30
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   405 AA;  47040 MW;  4805F2DAD0588232 CRC64;
     MDINKPGWNQ SDQQATAYDP NQQQYYGDGS TYYDPDQAVD PNQAYYPDPN TYPDAAAYYG
     YGQDGQAYPQ DYAQDPNQAY YADPNAYQDP NAYTDPNAYV DPNAYQDPNA YVDPNNYTDP
     NAYYGYGQDG QAYPQDYAQD PNQAYYADPN AYQDPNAYTD PYYVTSTDPN AYYGQVDNVP
     ALEASDLAYE VTPQEQAAEQ ELFSEPETKV IREIHEFPFE KIRSYFQTDF DSYNSRLTQL
     KDKLDNAIFS MRKAIDTVKE NSANLQIMKQ NFERQLKEQQ TQRLTSNTDA EKIGAKINQL
     EERMQRLSRT MESVEWTKKE PRQEQFDPRF VDPRNFNNYV NNTDTMMSMF EKVLMMNLLR
     STTPVQPPVQ YFTPQPLTAS PRPVYEEPIS ASFRRRGYRG DEFYE