ID   P69_NPVAC               Reviewed;          55 AA.
AC   P06545;
DT   01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   29-SEP-2021, entry version 66.
DE   RecName: Full=DNA-binding protein;
DE   AltName: Full=Arginine-rich protein 6.9 kDa;
DE   AltName: Full=Basic viral core protein;
DE   AltName: Full=Nucleocapsid protein;
GN   Name=P6.9; ORFNames=ORF100;
OS   Autographa californica nuclear polyhedrosis virus (AcMNPV).
OC   Viruses; Naldaviricetes; Lefavirales; Baculoviridae; Alphabaculovirus.
OX   NCBI_TaxID=46015;
OH   NCBI_TaxID=7088; Lepidoptera (butterflies and moths).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=L1;
RX   PubMed=3543402; DOI=10.1128/jvi.61.3.661-666.1987;
RA   Wilson M.E., Mainprize T.H., Friesen P.D., Miller L.K.;
RT   "Location, transcription, and sequence of a baculovirus gene encoding a
RT   small arginine-rich polypeptide.";
RL   J. Virol. 61:661-666(1987).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C6;
RX   PubMed=8030224; DOI=10.1006/viro.1994.1380;
RA   Ayres M.D., Howard S.C., Kuzio J., Lopez-Ferber M., Possee R.D.;
RT   "The complete DNA sequence of Autographa californica nuclear polyhedrosis
RT   virus.";
RL   Virology 202:586-605(1994).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 12-55.
RC   STRAIN=HR3;
RX   PubMed=1994581; DOI=10.1016/0042-6822(91)90500-b;
RA   Lu A., Carstens E.B.;
RT   "Nucleotide sequence of a gene essential for viral DNA replication in the
RT   baculovirus Autographa californica nuclear polyhedrosis virus.";
RL   Virology 181:336-347(1991).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-31.
RC   STRAIN=HR3;
RA   Lu A., Craig A., Carstens E.B.;
RL   Submitted (JUN-1994) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   FUNCTION.
RX   PubMed=3055664; DOI=10.1016/0042-6822(88)90073-6;
RA   Wilson M.E., Price K.H.;
RT   "Association of Autographa californica nuclear polyhedrosis virus (AcMNPV)
RT   with the nuclear matrix.";
RL   Virology 167:233-241(1988).
RN   [6]
RP   PHOSPHORYLATION, AND SUBCELLULAR LOCATION.
RX   PubMed=22951830; DOI=10.1128/jvi.00438-12;
RA   Liu X., Zhao H., Fang Z., Yuan M., Yang K., Pang Y.;
RT   "Distribution and phosphorylation of the basic protein P6.9 of Autographa
RT   californica nucleopolyhedrovirus.";
RL   J. Virol. 86:12217-12227(2012).
RN   [7]
RP   INTERACTION WITH AC132.
RX   PubMed=25142609; DOI=10.1128/jvi.01313-14;
RA   Yang M., Wang S., Yue X.L., Li L.L.;
RT   "Autographa californica multiple nucleopolyhedrovirus orf132 encodes a
RT   nucleocapsid-associated protein required for budded-virus and multiply
RT   enveloped occlusion-derived virus production.";
RL   J. Virol. 88:12586-12598(2014).
CC   -!- FUNCTION: Plays a role in viral DNA packaging and nucleocapsid
CC       assembly. Promotes viral gene transcription during the late stage of
CC       infection while it is non-essential for the basal level of viral gene
CC       transcription. {ECO:0000269|PubMed:3055664}.
CC   -!- SUBUNIT: Interacts with protein AC132. {ECO:0000269|PubMed:25142609}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000269|PubMed:22951830}. Host
CC       cytoplasm {ECO:0000269|PubMed:22951830}. Note=Within the host cell, the
CC       majority of P6.9 is distributed near the inner nuclear membrane.
CC       Present in both the budded virus (BV) and the occluded virus (OV). The
CC       occluded form allows the virus to be transmitted from insect to insect
CC       through ingestion of contaminated food while the budded form is
CC       responsible for the dissemination of infection throughout the insect
CC       host. {ECO:0000269|PubMed:22951830}.
CC   -!- PTM: Phosphorylated. {ECO:0000269|PubMed:22951830}.
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DR   EMBL; M15370; AAA46745.1; -; Genomic_DNA.
DR   EMBL; M57687; AAA67905.1; -; Genomic_DNA.
DR   EMBL; L22858; AAA66730.1; -; Genomic_DNA.
DR   EMBL; U10885; AAB08766.1; -; Genomic_DNA.
DR   PIR; A26593; VHNVAC.
DR   RefSeq; NP_054130.1; NC_001623.1.
DR   GeneID; 1403933; -.
DR   KEGG; vg:1403933; -.
DR   Proteomes; UP000008292; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0044204; C:host cell nuclear matrix; IDA:UniProtKB.
DR   GO; GO:0019013; C:viral nucleocapsid; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   DNA-binding; Host cytoplasm; Late protein; Phosphoprotein;
KW   Reference proteome; Repeat; Transcription; Transcription regulation;
KW   Viral nucleoprotein; Virion.
FT   INIT_MET        1
FT                   /note="Removed; by host"
FT                   /evidence="ECO:0000250"
FT   CHAIN           2..55
FT                   /note="DNA-binding protein"
FT                   /id="PRO_0000132807"
FT   REPEAT          5..10
FT                   /note="1"
FT   REPEAT          19..24
FT                   /note="2"
FT   REGION          1..55
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          5..24
FT                   /note="2 X 6 AA repeats of R-R-R-R-S-S"
FT   COMPBIAS        21..55
FT                   /note="Basic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   55 AA;  6885 MW;  BFF65E6721F1D568 CRC64;
     MVYRRRRRSS TGTTYGSTRR RRSSGYRRRP GRPRTYRRSR SRSSTGRRSY RTRYY