ID   P7_BPPH6                Reviewed;         161 AA.
AC   P11123;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1989, sequence version 1.
DT   29-SEP-2021, entry version 65.
DE   RecName: Full=Assembly protein P7;
GN   Name=P7;
OS   Pseudomonas phage phi6 (Bacteriophage phi-6).
OC   Viruses; Riboviria; Orthornavirae; Duplornaviricota; Vidaverviricetes;
OC   Mindivirales; Cystoviridae; Cystovirus.
OX   NCBI_TaxID=10879;
OH   NCBI_TaxID=319; Pseudomonas savastanoi pv. phaseolicola (Pseudomonas syringae pv. phaseolicola).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA], AND PARTIAL PROTEIN SEQUENCE.
RX   PubMed=3346944; DOI=10.1128/jvi.62.4.1180-1185.1988;
RA   Mindich L., Nemhauser I., Gottlieb P., Romantschuk M., Carton J.,
RA   Frucht S., Strassman J., Bamford D.H., Kalkkinen N.;
RT   "Nucleotide sequence of the large double-stranded RNA segment of
RT   bacteriophage phi 6: genes specifying the viral replicase and
RT   transcriptase.";
RL   J. Virol. 62:1180-1185(1988).
RN   [2]
RP   FUNCTION.
RX   PubMed=9086268; DOI=10.1006/jmbi.1996.0817;
RA   Juuti J.T., Bamford D.H.;
RT   "Protein P7 of phage phi6 RNA polymerase complex, acquiring of RNA
RT   packaging activity by in vitro assembly of the purified protein onto
RT   deficient particles.";
RL   J. Mol. Biol. 266:891-900(1997).
RN   [3]
RP   SUBUNIT, AND FUNCTION.
RX   PubMed=18793644; DOI=10.1016/j.jmb.2008.08.082;
RA   Poranen M.M., Butcher S.J., Simonov V.M., Laurinmaki P., Bamford D.H.;
RT   "Roles of the minor capsid protein P7 in the assembly and replication of
RT   double-stranded RNA bacteriophage phi6.";
RL   J. Mol. Biol. 383:529-538(2008).
RN   [4]
RP   SUBCELLULAR LOCATION, AND FUNCTION.
RX   PubMed=22896624; DOI=10.1128/jvi.01347-12;
RA   Nemecek D., Qiao J., Mindich L., Steven A.C., Heymann J.B.;
RT   "Packaging accessory protein P7 and polymerase P2 have mutually occluding
RT   binding sites inside the bacteriophage 6 procapsid.";
RL   J. Virol. 86:11616-11624(2012).
RN   [5]
RP   STRUCTURE BY ELECTRON MICROSCOPY OF VIRAL PARTICLE, SUBCELLULAR LOCATION,
RP   FUNCTION, INTERACTION WITH RDRP, AND IDENTIFICATION IN THE PACKAGING
RP   COMPLEX.
RX   PubMed=23077625; DOI=10.1371/journal.pone.0047489;
RA   Katz G., Wei H., Alimova A., Katz A., Morgan D.G., Gottlieb P.;
RT   "Protein P7 of the cystovirus phi6 is located at the three-fold axis of the
RT   unexpanded procapsid.";
RL   PLoS ONE 7:E47489-E47489(2012).
CC   -!- FUNCTION: Assembly protein part of the packaging complex that packages
CC       the viral RNA segments, replicate them into a double-stranded form and
CC       transcribe them. Required for efficient procapsid assembly. Necessary
CC       for stable packaging. May stabilize the RNA-dependent RNA polymerase
CC       (RdRP) in its position at the three-fold axis on the inner side of
CC       empty-unexpanded procapsids. Could play a role in viral RNA
CC       recognition. Seems to be involved in the regulation of plus strand
CC       synthesis (transcription) as a fidelity factor.
CC       {ECO:0000269|PubMed:18793644, ECO:0000269|PubMed:22896624,
CC       ECO:0000269|PubMed:23077625, ECO:0000269|PubMed:9086268}.
CC   -!- SUBUNIT: Homodimer. Part of the packaging complex composed of RDRP, P4
CC       and P7. Interacts with RDRP (Probable). {ECO:0000305|PubMed:18793644,
CC       ECO:0000305|PubMed:23077625}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000305}. Note=Found in the capsid
CC       (Probable). Prior to RNA packaging, localizes on the inner procapsid
CC       surface near the three-fold axis of symmetry.
CC       {ECO:0000269|PubMed:22896624, ECO:0000269|PubMed:23077625,
CC       ECO:0000305}.
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DR   EMBL; M17461; AAA32354.1; -; Genomic_RNA.
DR   PIR; A29885; P7BPF6.
DR   RefSeq; NP_620345.1; NC_003715.1.
DR   GeneID; 956435; -.
DR   KEGG; vg:956435; -.
DR   Proteomes; UP000002610; Genome.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Reference proteome; Transcription;
KW   Viral capsid assembly; Viral genome packaging;
KW   Viral release from host cell; Virion.
FT   CHAIN           1..161
FT                   /note="Assembly protein P7"
FT                   /id="PRO_0000164641"
SQ   SEQUENCE   161 AA;  17300 MW;  2EA152DB04B65C3F CRC64;
     MTLYLVPPLD SADKELPALA SKAGVTLLEI EFLHELWPHL SGGQIVIAAL NANNLAILNR
     HMSTLLVELP VAVMAVPGAS YRSDWNMIAH ALPSEDWITL SNKMLKSGLL ANDTVQGEKR
     SGAEPLSPNV YTDALSRLGI ATAHAIPVEP EQPFDVDEVS A