ID   P8_RDVF                 Reviewed;         421 AA.
AC   Q85439;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   12-AUG-2020, entry version 82.
DE   RecName: Full=Outer capsid protein P8;
DE   AltName: Full=Structural protein P8;
DE   Contains:
DE     RecName: Full=Outer capsid protein P8';
DE   Contains:
DE     RecName: Full=Small peptide 1;
DE              Short=Sp1;
OS   Rice dwarf virus (isolate Fujian) (RDV).
OC   Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes;
OC   Reovirales; Reoviridae; Sedoreovirinae; Phytoreovirus.
OX   NCBI_TaxID=142804;
OH   NCBI_TaxID=114194; Alopecurus aequalis.
OH   NCBI_TaxID=90397; Echinochloa crus-galli (Barnyard grass) (Panicum crus-galli).
OH   NCBI_TaxID=94400; Nephotettix cincticeps (Green rice leafhopper) (Selenocephalus cincticeps).
OH   NCBI_TaxID=4530; Oryza sativa (Rice).
OH   NCBI_TaxID=147271; Paspalum.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Li W., Li Y., Zhang X., Chu R., Chen Z.;
RT   "Molecular cloning and sequencing of the eighth largest segment of rice
RT   dwarf virus and its expression in E. coli.";
RL   Ping Tu Hsueh Pao 11:56-62(1995).
RN   [2]
RP   PARTIAL NUCLEOTIDE SEQUENCE [MRNA].
RA   Gao Q., Qu Y., Liu W., Pan N.-S., Chen Z.;
RT   "Partial cDNA cloning and nucleotide sequence of rice dwarf virus genome.";
RL   Acta Bot. Sin. 32:13-18(1990).
RN   [3]
RP   PROTEOLYTIC PROCESSING, AND PARTIAL PROTEIN SEQUENCE.
RX   PubMed=9787666; DOI=10.1007/s007050050421;
RA   Mao Z.J., Li Y., Xu H., Zheng H.H., Schiemann J., Casper R., Chen Z.L.;
RT   "The 42K protein of rice dwarf virus is a post-translational cleavage
RT   product of the 46K outer capsid protein.";
RL   Arch. Virol. 143:1831-1838(1998).
RN   [4]
RP   SUBCELLULAR LOCATION.
RX   PubMed=16432031; DOI=10.1099/vir.0.81425-0;
RA   Wei T., Shimizu T., Hagiwara K., Kikuchi A., Moriyasu Y., Suzuki N.,
RA   Chen H., Omura T.;
RT   "Pns12 protein of Rice dwarf virus is essential for formation of viroplasms
RT   and nucleation of viral-assembly complexes.";
RL   J. Gen. Virol. 87:429-438(2006).
RN   [5]
RP   INTERACTION WITH ORYZA SATIVA GLO1/GOX, AND SUBCELLULAR LOCATION.
RC   STRAIN=cv. Xiushui 11;
RX   PubMed=17174956; DOI=10.1016/j.febslet.2006.11.073;
RA   Zhou F., Wu G., Deng W., Pu Y., Wei C., Li Y.;
RT   "Interaction of rice dwarf virus outer capsid P8 protein with rice
RT   glycolate oxidase mediates relocalization of P8.";
RL   FEBS Lett. 581:34-40(2007).
CC   -!- FUNCTION: Capsid protein which self-assembles to form the outer
CC       icosahedral capsid with a T=13 symmetry, about 70 nm in diameter and
CC       consisting of 780 molecules capsid proteins.
CC   -!- SUBUNIT: Homotrimer (By similarity). Homomultimer (By similarity).
CC       Interacts with host peroxisomal glycolate oxidase (GOX). This
CC       interaction mediates its relocation to virus factories peripheral to
CC       host peroxisomes. {ECO:0000250, ECO:0000269|PubMed:17174956}.
CC   -!- SUBCELLULAR LOCATION: [Outer capsid protein P8]: Virion {ECO:0000305}.
CC       Host cytoplasm {ECO:0000269|PubMed:16432031,
CC       ECO:0000269|PubMed:17174956}. Note=Found in the peripheral regions of
CC       spherical cytoplasmic structures, called virus factories, that appear
CC       early after infection and are the site of viral replication and
CC       packaging.
CC   -!- SIMILARITY: Belongs to the phytoreovirus outer capsid protein P8
CC       family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; U36565; AAA88764.1; -; mRNA.
DR   RefSeq; NP_620534.1; NC_003764.1.
DR   SMR; Q85439; -.
DR   GeneID; 956499; -.
DR   KEGG; vg:956499; -.
DR   Proteomes; UP000002239; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IDA:UniProtKB.
DR   GO; GO:0044161; C:host cell cytoplasmic vesicle; IDA:UniProtKB.
DR   GO; GO:0019031; C:viral envelope; IEA:InterPro.
DR   GO; GO:0039624; C:viral outer capsid; IEA:UniProtKB-KW.
DR   GO; GO:0046789; F:host cell surface receptor binding; IEA:InterPro.
DR   GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR   GO; GO:0019064; P:fusion of virus membrane with host plasma membrane; IEA:InterPro.
DR   GO; GO:0019048; P:modulation by virus of host process; IPI:UniProtKB.
DR   InterPro; IPR008980; Capsid_hemagglutn.
DR   InterPro; IPR009807; Phytoreo_P8.
DR   InterPro; IPR008935; Virus_capsid_a-hlx_vir.
DR   Pfam; PF07124; Phytoreo_P8; 1.
DR   SUPFAM; SSF48345; SSF48345; 1.
DR   SUPFAM; SSF49818; SSF49818; 1.
PE   1: Evidence at protein level;
KW   Capsid protein; Direct protein sequencing; Host cytoplasm;
KW   Host-virus interaction; Outer capsid protein; Reference proteome; Virion.
FT   CHAIN           1..421
FT                   /note="Outer capsid protein P8"
FT                   /id="PRO_0000040671"
FT   CHAIN           1..362
FT                   /note="Outer capsid protein P8'"
FT                   /id="PRO_0000040672"
FT   CHAIN           363..421
FT                   /note="Small peptide 1"
FT                   /id="PRO_0000040673"
SQ   SEQUENCE   421 AA;  46424 MW;  E1B6E34C52FEE235 CRC64;
     MSRQMWLDTS ALLEAISEYV VRCNGDTFSG LTTGDFNALS NMFTQLSVSS AGYVSDPRVP
     LQTMSNMFVS FITSTDRCGY MLGKTWFNSD TKPTVSDDFI TAYIKPRLQV PMSDTVRQLN
     NLSLQPSAKP KLYERQNAIM KGLDIPYSEP IEPCKLFRSV AGQTGNIPLM GILLTPPVAQ
     QQPFFVAERR RILFGIRSNA AIPAGAYQFV VPAWASVLSV TGAYVYFTNS FFGTTIAGVT
     ATATAADAAT TFTVPTDANN LPVQTDSRLS FSLGGGNINL ELGVAKTGFC VAIEGEFTIL
     ANRSQAYYTL NSITQTPTSI DDFDVSDFLT TFLSQLRACG QYEIFSDAMD QLTNNLITNY
     MDPPAIPAGL AFTSPWFRFS ERARTILALQ NVDLNIRKLM VRHLWVITSL IAVFGRYYRP
     N