PA1L_PSEAE
ID PA1L_PSEAE Reviewed; 122 AA.
AC Q05097;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=PA-I galactophilic lectin;
DE Short=PA-IL;
DE AltName: Full=Galactose-binding lectin;
GN Name=lecA; Synonyms=pa1L; OrderedLocusNames=PA2570;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 2-31.
RC STRAIN=ATCC 33347;
RX PubMed=1429650; DOI=10.1016/s0021-9258(18)50050-8;
RA Avichezer D., Katcoff D.J., Garber N.C., Gilboa-Garber N.;
RT "Analysis of the amino acid sequence of the Pseudomonas aeruginosa
RT galactophilic PA-I lectin.";
RL J. Biol. Chem. 267:23023-23027(1992).
RN [2]
RP SEQUENCE REVISION.
RX PubMed=8193158; DOI=10.1016/0167-4781(94)90095-7;
RA Avichezer D., Gilboa-Garber N., Garber N.C., Katcoff D.J.;
RT "Pseudomonas aeruginosa PA-I lectin gene molecular analysis and expression
RT in Escherichia coli.";
RL Biochim. Biophys. Acta 1218:11-20(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Winzer K., Falconer C., Diggle S.P., Garber N.C., Camara M., Williams P.;
RT "The Pseudomonas aeruginosa lectins PA-1L and PA-2L are controlled by
RT quorum sensing and by RpoS.";
RL Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- FUNCTION: D-galactose specific lectin. Binds in decreasing order of
CC affinity: melibiose, methyl-alpha-D-galactoside, D-galactose, methyl-
CC beta-D-galactoside, N-acetyl-D-galactosamine. Similar to plant lectins
CC in its selective (carbohydrate-specific) hemagglutinating activity.
CC -!- SUBCELLULAR LOCATION: Cytoplasm. Periplasm. Cell surface. Note=Low
CC exposure on the cell surface.
CC -!- SIMILARITY: Belongs to the LecA/PllA lectin family. {ECO:0000305}.
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DR EMBL; X65933; CAA46728.1; -; Genomic_DNA.
DR EMBL; AF229814; AAF70459.1; -; Genomic_DNA.
DR EMBL; AE004091; AAG05958.1; -; Genomic_DNA.
DR PIR; S45300; S45300.
DR RefSeq; NP_251260.1; NC_002516.2.
DR RefSeq; WP_003102492.1; NZ_QZGE01000008.1.
DR PDB; 1L7L; X-ray; 1.50 A; A=2-122.
DR PDB; 1OKO; X-ray; 1.60 A; A/B/C/D=2-122.
DR PDB; 1UOJ; X-ray; 2.40 A; A/B/C/D=2-122.
DR PDB; 2VXJ; X-ray; 1.90 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X=2-122.
DR PDB; 2WYF; X-ray; 2.40 A; A/B/C/D/E/F/G/H=2-122.
DR PDB; 3ZYB; X-ray; 2.29 A; A/B/C/D/E/F/G/H=1-122.
DR PDB; 3ZYF; X-ray; 1.94 A; A/B/C/D=1-122.
DR PDB; 3ZYH; X-ray; 1.50 A; A/B=1-122.
DR PDB; 4A6S; X-ray; 2.15 A; A/B/C/D=2-122.
DR PDB; 4AL9; X-ray; 1.75 A; A/B/C/D/E/F/G/H=2-122.
DR PDB; 4CP9; X-ray; 1.65 A; A/B/C/D=2-122.
DR PDB; 4CPB; X-ray; 1.57 A; A/B/C/D=2-122.
DR PDB; 4LJH; X-ray; 1.45 A; A/B/C/D=1-122.
DR PDB; 4LK6; X-ray; 2.86 A; A/B/C/D/E/F/G/H/I/J/K/L=2-122.
DR PDB; 4LK7; X-ray; 1.76 A; A/B/C/D=2-122.
DR PDB; 4LKD; X-ray; 2.31 A; A/B/C/D/E/F/G/H=2-122.
DR PDB; 4LKE; X-ray; 1.65 A; A/B/C/D=2-122.
DR PDB; 4LKF; X-ray; 1.64 A; A/B=2-122.
DR PDB; 4YW6; X-ray; 1.40 A; A/B/C/D=2-122.
DR PDB; 4YW7; X-ray; 1.82 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P=2-122.
DR PDB; 4YWA; X-ray; 1.19 A; A/B/C/D=2-122.
DR PDB; 5D21; X-ray; 1.90 A; A/B/C/D=2-122.
DR PDB; 5MIH; X-ray; 1.80 A; A/B/C/D=2-122.
DR PDB; 6YO3; X-ray; 1.84 A; A/B/C/D=2-122.
DR PDB; 6YOH; X-ray; 1.84 A; A/B/C/D=2-122.
DR PDB; 7FIO; X-ray; 1.50 A; A/B/C/D=2-122.
DR PDBsum; 1L7L; -.
DR PDBsum; 1OKO; -.
DR PDBsum; 1UOJ; -.
DR PDBsum; 2VXJ; -.
DR PDBsum; 2WYF; -.
DR PDBsum; 3ZYB; -.
DR PDBsum; 3ZYF; -.
DR PDBsum; 3ZYH; -.
DR PDBsum; 4A6S; -.
DR PDBsum; 4AL9; -.
DR PDBsum; 4CP9; -.
DR PDBsum; 4CPB; -.
DR PDBsum; 4LJH; -.
DR PDBsum; 4LK6; -.
DR PDBsum; 4LK7; -.
DR PDBsum; 4LKD; -.
DR PDBsum; 4LKE; -.
DR PDBsum; 4LKF; -.
DR PDBsum; 4YW6; -.
DR PDBsum; 4YW7; -.
DR PDBsum; 4YWA; -.
DR PDBsum; 5D21; -.
DR PDBsum; 5MIH; -.
DR PDBsum; 6YO3; -.
DR PDBsum; 6YOH; -.
DR PDBsum; 7FIO; -.
DR AlphaFoldDB; Q05097; -.
DR SMR; Q05097; -.
DR STRING; 287.DR97_5600; -.
DR BindingDB; Q05097; -.
DR ChEMBL; CHEMBL3351196; -.
DR UniLectin; Q05097; -.
DR PaxDb; Q05097; -.
DR PRIDE; Q05097; -.
DR EnsemblBacteria; AAG05958; AAG05958; PA2570.
DR GeneID; 882335; -.
DR KEGG; pae:PA2570; -.
DR PseudoCAP; PA2570; -.
DR HOGENOM; CLU_162760_0_0_6; -.
DR OMA; KIESRYD; -.
DR BioCyc; PAER208964:G1FZ6-2606-MON; -.
DR EvolutionaryTrace; Q05097; -.
DR PRO; PR:Q05097; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0009986; C:cell surface; IEA:UniProtKB-SubCell.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR GO; GO:0030246; F:carbohydrate binding; IEA:UniProtKB-KW.
DR GO; GO:0007157; P:heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules; IDA:PseudoCAP.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR012905; PA-IL.
DR Pfam; PF07828; PA-IL; 1.
DR PIRSF; PIRSF020485; PA-IL; 1.
DR SUPFAM; SSF49785; SSF49785; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Direct protein sequencing; Lectin; Periplasm;
KW Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:1429650"
FT CHAIN 2..122
FT /note="PA-I galactophilic lectin"
FT /id="PRO_0000058158"
FT STRAND 3..8
FT /evidence="ECO:0007829|PDB:4YWA"
FT STRAND 15..21
FT /evidence="ECO:0007829|PDB:4YWA"
FT STRAND 27..41
FT /evidence="ECO:0007829|PDB:4YWA"
FT STRAND 46..48
FT /evidence="ECO:0007829|PDB:4LKF"
FT STRAND 65..70
FT /evidence="ECO:0007829|PDB:4YWA"
FT STRAND 81..85
FT /evidence="ECO:0007829|PDB:4YWA"
FT STRAND 93..99
FT /evidence="ECO:0007829|PDB:4YWA"
FT HELIX 106..108
FT /evidence="ECO:0007829|PDB:4YWA"
FT STRAND 110..119
FT /evidence="ECO:0007829|PDB:4YWA"
SQ SEQUENCE 122 AA; 12893 MW; 1DC739390388A451 CRC64;
MAWKGEVLAN NEAGQVTSII YNPGDVITIV AAGWASYGPT QKWGPQGDRE HPDQGLICHD
AFCGALVMKI GNSGTIPVNT GLFRWVAPNN VQGAITLIYN DVPGTYGNNS GSFSVNIGKD
QS
