ASIC4_RAT
ID ASIC4_RAT Reviewed; 539 AA.
AC Q9JHS6; Q9QYV9;
DT 13-SEP-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 25-MAY-2022, entry version 94.
DE RecName: Full=Acid-sensing ion channel 4;
DE Short=ASIC4;
DE AltName: Full=Amiloride-sensitive cation channel 4;
DE AltName: Full=Spinal cord ASIC;
GN Name=Asic4; Synonyms=Accn4, Spasic;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Brain;
RX PubMed=10852210; DOI=10.1097/00001756-200006050-00003;
RA Gruender S., Geisler H.-S., Baessler E.-L., Ruppersberg J.P.;
RT "A new member of acid-sensing ion channels from pituitary gland.";
RL NeuroReport 11:1607-1611(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND
RP FUNCTION.
RC TISSUE=Spinal ganglion;
RX PubMed=10923674; DOI=10.1097/00001756-200007140-00031;
RA Akopian A.N., Chen C.-C., Ding Y., Cesare P., Wood J.N.;
RT "A new member of the acid-sensing ion channel family.";
RL NeuroReport 11:2217-2222(2000).
CC -!- FUNCTION: Probable cation channel with high affinity for sodium.
CC {ECO:0000269|PubMed:10923674}.
CC -!- SUBUNIT: Homotrimer or heterotrimer with other ASIC proteins.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in brain, spinal cord and dorsal root
CC ganglion (DRG). Expressed by a subset of sensory neurons in the DRG.
CC Expressed by granule cells in the cerebellar cortex. In hippocampus,
CC expression is detected in dentate gyrus granule cells, in pyramidal
CC cells of CA1-CA3 subfields and in interneurons of the striatum oriens
CC and radiatum of all subfields. In cerebral cortex expressed in small,
CC medium and large pyramidal cells in layers 2, 3 and 5 respectively.
CC Also expressed in striatum, globus pallidus, inferior and superior
CC calliculi, amygdala, magnocellular preoptic nucleus, islands of Calleja
CC and large neurons of olfactory tubercules.
CC {ECO:0000269|PubMed:10923674}.
CC -!- DEVELOPMENTAL STAGE: Highly expressed in newborn spinal cord but hardly
CC detected in the cerebellum compared to adult. Expressed at postnatal
CC day 1 in ependymal cells lining the central canal of spinal cord and in
CC motor neurons. In adult, expression decreases in ependymal cells and
CC increases in motor neurons. The number of positive interneurons
CC decreases but the individual interneuron expression increases in adult
CC spinal cord compared to newborn. {ECO:0000269|PubMed:10923674}.
CC -!- MISCELLANEOUS: In vitro, has no proton-gated channel activity.
CC -!- SIMILARITY: Belongs to the amiloride-sensitive sodium channel (TC
CC 1.A.6) family. ASIC4 subfamily. {ECO:0000305}.
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DR EMBL; AJ271642; CAB93984.1; -; mRNA.
DR EMBL; AJ242554; CAB61836.1; -; mRNA.
DR RefSeq; NP_071570.2; NM_022234.2.
DR AlphaFoldDB; Q9JHS6; -.
DR SMR; Q9JHS6; -.
DR STRING; 10116.ENSRNOP00000027135; -.
DR GlyGen; Q9JHS6; 4 sites.
DR PaxDb; Q9JHS6; -.
DR GeneID; 63882; -.
DR KEGG; rno:63882; -.
DR UCSC; RGD:621068; rat.
DR CTD; 55515; -.
DR RGD; 621068; Asic4.
DR eggNOG; KOG4294; Eukaryota.
DR InParanoid; Q9JHS6; -.
DR OrthoDB; 686369at2759; -.
DR PhylomeDB; Q9JHS6; -.
DR Reactome; R-RNO-2672351; Stimuli-sensing channels.
DR PRO; PR:Q9JHS6; -.
DR Proteomes; UP000002494; Unplaced.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0015280; F:ligand-gated sodium channel activity; IBA:GO_Central.
DR GO; GO:0001662; P:behavioral fear response; ISO:RGD.
DR GO; GO:0035725; P:sodium ion transmembrane transport; IBA:GO_Central.
DR InterPro; IPR001873; ENaC.
DR InterPro; IPR020903; ENaC_CS.
DR PANTHER; PTHR11690; PTHR11690; 1.
DR Pfam; PF00858; ASC; 1.
DR PRINTS; PR01078; AMINACHANNEL.
DR PROSITE; PS01206; ASC; 1.
PE 2: Evidence at transcript level;
KW Disulfide bond; Glycoprotein; Ion channel; Ion transport; Membrane;
KW Reference proteome; Sodium; Sodium channel; Sodium transport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..539
FT /note="Acid-sensing ion channel 4"
FT /id="PRO_0000181306"
FT TOPO_DOM 1..68
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 69..89
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 90..438
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 439..459
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 460..539
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 501..531
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 191
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 243
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 341
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 376
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 118..202
FT /evidence="ECO:0000250"
FT DISULFID 180..187
FT /evidence="ECO:0000250"
FT DISULFID 296..375
FT /evidence="ECO:0000250"
FT DISULFID 318..371
FT /evidence="ECO:0000250"
FT DISULFID 322..369
FT /evidence="ECO:0000250"
FT DISULFID 331..353
FT /evidence="ECO:0000250"
FT DISULFID 333..345
FT /evidence="ECO:0000250"
FT CONFLICT 283
FT /note="K -> E (in Ref. 2; CAB61836)"
FT /evidence="ECO:0000305"
FT CONFLICT 401
FT /note="K -> R (in Ref. 2; CAB61836)"
FT /evidence="ECO:0000305"
FT CONFLICT 536
FT /note="D -> N (in Ref. 2; CAB61836)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 539 AA; 59325 MW; 3D73DE0EAAFF7097 CRC64;
MPIEIVCKIK FAEEDAKPKE KEAGDEQSLL GAAQGPAAPR DLATFASTST LHGLGRACGP
GPHGLRRTLW VLALLTSLAA FLYQAASLAR GYLTRPHLVA MDPAAPAPVA GFPAVTLCNI
NRFRHSALSD ADIFHLANLT GLPPKDRDGH RAAGLRYPEP DMVDILNRTG HQLADMLKSC
NFSGHHCSAS NFSVVYTRYG KCYTFNADPQ SSLPSRAGGM GSGLEIMLDI QQEEYLPIWR
ETNETSFEAG IRVQIHSQEE PPYIHQLGFG VSPGFQTFVS CQKQRLTYLP QPWGNCRAES
KLREPELQGY SAYSVSACRL RCEKEAVLQR CHCRMVHMPG NETICPPNIY IECADHTLDS
LGGGSEGPCF CPTPCNLTRY GKEISMVKIP NRGSARYLAR KYNRNETYIR ENFLVLDVFF
EALTSEAMEQ RAAYGLSALL GDLGGQMGLF IGASILTLLE ILDYIYEVSW DRLKRVWRRP
KTPLRTSTGG ISTLGLQELK EQSPCPNRGR AEGGGASNLL PNHHHPHGPP GSLFEDFAC
