ID   PA2B_LITPA              Reviewed;          31 AA.
AC   P84279; D3UA82;
DT   23-NOV-2004, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   25-MAY-2022, entry version 38.
DE   RecName: Full=Palustrin-2b;
DE   Flags: Fragment;
OS   Lithobates palustris (Pickerel frog) (Rana palustris).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Ranoidea; Ranidae; Lithobates.
OX   NCBI_TaxID=298395;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MASS
RP   SPECTROMETRY, AND DISULFIDE BOND.
RC   TISSUE=Skin secretion {ECO:0000269|PubMed:11087945};
RX   PubMed=11087945; DOI=10.1016/s0167-4838(00)00191-6;
RA   Basir Y.J., Knoop F.C., Dulka J., Conlon J.M.;
RT   "Multiple antimicrobial peptides and peptides related to bradykinin and
RT   neuromedin N isolated from skin secretions of the pickerel frog, Rana
RT   palustris.";
RL   Biochim. Biophys. Acta 1543:95-105(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=20179920; DOI=10.1007/s00251-010-0430-7;
RA   Tennessen J.A., Blouin M.S.;
RT   "A revised leopard frog phylogeny allows a more detailed examination of
RT   adaptive evolution at ranatuerin-2 antimicrobial peptide loci.";
RL   Immunogenetics 62:333-343(2010).
CC   -!- FUNCTION: Antimicrobial activity against Gram-negative bacterium
CC       E.coli. {ECO:0000269|PubMed:11087945}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:11087945}.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC       {ECO:0000269|PubMed:11087945}.
CC   -!- MASS SPECTROMETRY: Mass=3186.7; Mass_error=0.6; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:11087945};
CC   -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC       Brevinin subfamily. {ECO:0000269|PubMed:11087945}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; FJ845481; ACZ44729.1; -; Genomic_DNA.
DR   EMBL; FJ845482; ACZ44730.1; -; Genomic_DNA.
DR   AlphaFoldDB; P84279; -.
DR   SMR; P84279; -.
DR   GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR   GO; GO:0050829; P:defense response to Gram-negative bacterium; IDA:UniProtKB.
DR   InterPro; IPR012521; Antimicrobial_frog_2.
DR   Pfam; PF08023; Antimicrobial_2; 1.
PE   1: Evidence at protein level;
KW   Amphibian defense peptide; Antibiotic; Antimicrobial;
KW   Direct protein sequencing; Disulfide bond; Secreted.
FT   PEPTIDE         <1..31
FT                   /note="Palustrin-2b"
FT                   /id="PRO_0000044740"
FT   DISULFID        23..29
FT                   /evidence="ECO:0000269|PubMed:11087945"
FT   NON_TER         1
SQ   SEQUENCE   31 AA;  3189 MW;  8B41001DDBFB6439 CRC64;
     GFFSTVKNLA TNVAGTVIDT LKCKVTGGCR S