ID   PA2N_DABRR              Reviewed;          19 AA.
AC   P0DKX1;
DT   06-FEB-2013, integrated into UniProtKB/Swiss-Prot.
DT   06-FEB-2013, sequence version 1.
DT   25-MAY-2022, entry version 20.
DE   RecName: Full=Neutral phospholipase A2 RVV-PFIIc';
DE            Short=svPLA2;
DE            EC=3.1.1.4;
DE   AltName: Full=Phosphatidylcholine 2-acylhydrolase;
DE   Flags: Fragment;
OS   Daboia russelii (Russel's viper) (Vipera russelii).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC   Serpentes; Colubroidea; Viperidae; Viperinae; Daboia.
OX   NCBI_TaxID=8707;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, AND TOXIC DOSE.
RC   STRAIN=Eastern India; TISSUE=Venom;
RX   PubMed=12236591; DOI=10.1023/a:1016591318855;
RA   Chakraborty A.K., Hall R.H., Ghose A.C.;
RT   "Purification and characterization of a potent hemolytic toxin with
RT   phospholipase A2 activity from the venom of Indian Russell's viper.";
RL   Mol. Cell. Biochem. 237:95-102(2002).
CC   -!- FUNCTION: Snake venom phospholipase A2 (PLA2) that shows enzymatic,
CC       indirect hemolytic and anticoagulant activities. Interestingly, anti-
CC       PFIIc'serum completely inhibits anticoagulant activity, partially
CC       inhibits the indirect hemolytic activity, but does not inhibit
CC       enzymatic activity. PLA2 catalyzes the calcium-dependent hydrolysis of
CC       the 2-acyl groups in 3-sn-phosphoglycerides.
CC       {ECO:0000269|PubMed:12236591}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-
CC         glycero-3-phosphocholine + a fatty acid + H(+); Xref=Rhea:RHEA:15801,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:28868,
CC         ChEBI:CHEBI:57643, ChEBI:CHEBI:58168; EC=3.1.1.4;
CC   -!- COFACTOR:
CC       Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence={ECO:0000250};
CC       Note=Binds 1 Ca(2+) ion. {ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC   -!- PTM: Contains 7 disulfide bonds. {ECO:0000250}.
CC   -!- TOXIC DOSE: LD(50) is 0.1 mg/kg by intraperitoneal injection.
CC       {ECO:0000269|PubMed:12236591}.
CC   -!- MISCELLANEOUS: This phospholipase A2 is distinct from phospholipases of
CC       Russel's viper venom reported from other parts of India.
CC       {ECO:0000305|PubMed:12236591}.
CC   -!- SIMILARITY: Belongs to the phospholipase A2 family. Group II subfamily.
CC       {ECO:0000305}.
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DR   AlphaFoldDB; P0DKX1; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004623; F:phospholipase A2 activity; IEA:UniProtKB-EC.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0044179; P:hemolysis in another organism; IEA:UniProtKB-KW.
DR   GO; GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Blood coagulation cascade inhibiting toxin; Calcium; Cytolysis;
KW   Direct protein sequencing; Disulfide bond; Hemolysis;
KW   Hemostasis impairing toxin; Hydrolase; Lipid degradation; Lipid metabolism;
KW   Metal-binding; Secreted; Toxin.
FT   CHAIN           1..>19
FT                   /note="Neutral phospholipase A2 RVV-PFIIc'"
FT                   /id="PRO_0000421163"
FT   NON_TER         19
SQ   SEQUENCE   19 AA;  2194 MW;  A21F2F35DF79323C CRC64;
     NLFQFAEMIV KMTGKEAVH