ID   PA2_CARAE               Reviewed;          12 AA.
AC   P86517;
DT   15-JUN-2010, integrated into UniProtKB/Swiss-Prot.
DT   15-JUN-2010, sequence version 1.
DT   23-FEB-2022, entry version 15.
DE   RecName: Full=Phospholipase A2 {ECO:0000303|PubMed:19695876};
DE            EC=3.1.1.4;
DE   Flags: Fragment;
OS   Carcinus aestuarii (Green crab) (Carcinus mediterraneus).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Crustacea; Multicrustacea;
OC   Malacostraca; Eumalacostraca; Eucarida; Decapoda; Pleocyemata; Brachyura;
OC   Eubrachyura; Portunoidea; Carcinidae; Carcinus.
OX   NCBI_TaxID=53602;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, CATALYTIC ACTIVITY, COFACTOR, AND BIOPHYSICOCHEMICAL
RP   PROPERTIES.
RC   TISSUE=Hepatopancreas {ECO:0000269|PubMed:19695876};
RX   PubMed=19695876; DOI=10.1016/j.biortech.2009.07.031;
RA   Cherif S., Ben Bacha A., Ben Ali Y., Horchani H., Rekik W., Gargouri Y.;
RT   "Crab digestive phospholipase: a new invertebrate member.";
RL   Bioresour. Technol. 101:366-371(2010).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-
CC         glycero-3-phosphocholine + a fatty acid + H(+); Xref=Rhea:RHEA:15801,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:28868,
CC         ChEBI:CHEBI:57643, ChEBI:CHEBI:58168; EC=3.1.1.4;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10035, ECO:0000255|PROSITE-
CC         ProRule:PRU10036, ECO:0000269|PubMed:19695876};
CC   -!- COFACTOR:
CC       Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC         Evidence={ECO:0000269|PubMed:19695876};
CC       Note=Binds 1 Ca(2+) ion per subunit. {ECO:0000269|PubMed:19695876};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=40 mM for phosphatidylcholine (in the presence of 8 mM NaDC and 10
CC         mM CaCl(2), at 50 degrees Celsius and pH 8)
CC         {ECO:0000269|PubMed:19695876};
CC         Vmax=50 umol/min/mg enzyme toward phosphatidylcholine (in the
CC         presence of 8 mM NaDC and 10 mM CaCl(2), at 50 degrees Celsius and pH
CC         8) {ECO:0000269|PubMed:19695876};
CC       pH dependence:
CC         Optimum pH is 8.0. Stable between pH 6.0 and 9.0.
CC         {ECO:0000269|PubMed:19695876};
CC       Temperature dependence:
CC         Optimum temperature is 50 degrees Celsius. No activity at
CC         temperatures below 25 degrees Celsius or above 60 degrees Celsius.
CC         {ECO:0000269|PubMed:19695876};
CC   -!- SIMILARITY: Belongs to the phospholipase A2 family.
CC       {ECO:0000269|PubMed:19695876}.
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DR   GO; GO:0005509; F:calcium ion binding; IDA:UniProtKB.
DR   GO; GO:0004623; F:phospholipase A2 activity; IDA:UniProtKB.
DR   GO; GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Calcium; Direct protein sequencing; Hydrolase; Lipid degradation;
KW   Lipid metabolism; Metal-binding.
FT   CHAIN           1..>12
FT                   /note="Phospholipase A2"
FT                   /id="PRO_0000394410"
FT   NON_TER         12
FT                   /evidence="ECO:0000303|PubMed:19695876"
SQ   SEQUENCE   12 AA;  1191 MW;  301006411DA1ADD8 CRC64;
     XIGAGATSKL VY