PA71H_ARATH
ID PA71H_ARATH Reviewed; 359 AA.
AC Q9T0H9;
DT 05-OCT-2010, integrated into UniProtKB/Swiss-Prot.
DT 05-OCT-2010, sequence version 2.
DT 03-AUG-2022, entry version 99.
DE RecName: Full=Protein PAM71-homolog, chloroplastic {ECO:0000303|PubMed:27020959};
DE AltName: Full=GDT1-like protein 2 {ECO:0000305};
DE AltName: Full=PHOTOSYNTHESIS AFFECTED MUTANT71-homolog {ECO:0000303|PubMed:27020959};
DE Flags: Precursor;
GN Name=PAM71-HL {ECO:0000303|PubMed:27020959};
GN OrderedLocusNames=At4g13590 {ECO:0000312|Araport:AT4G13590};
GN ORFNames=T6G15.140 {ECO:0000312|EMBL:CAB41117.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14993207; DOI=10.1101/gr.1515604;
RA Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M.,
RA Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M.,
RA Weissenbach J., Salanoubat M.;
RT "Whole genome sequence comparisons and 'full-length' cDNA sequences: a
RT combined approach to evaluate and improve Arabidopsis genome annotation.";
RL Genome Res. 14:406-413(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION [LARGE SCALE
RP ANALYSIS].
RX PubMed=18431481; DOI=10.1371/journal.pone.0001994;
RA Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q.,
RA van Wijk K.J.;
RT "Sorting signals, N-terminal modifications and abundance of the chloroplast
RT proteome.";
RL PLoS ONE 3:E1994-E1994(2008).
RN [5]
RP IDENTIFICATION.
RX PubMed=27020959; DOI=10.1105/tpc.15.00812;
RA Schneider A., Steinberger I., Herdean A., Gandini C., Eisenhut M., Kurz S.,
RA Morper A., Hoecker N., Ruehle T., Labs M., Fluegge U.I., Geimer S.,
RA Schmidt S.B., Husted S., Weber A.P., Spetea C., Leister D.;
RT "The evolutionarily conserved protein PHOTOSYNTHESIS AFFECTED MUTANT71 is
RT required for efficient manganese uptake at the thylakoid membrane in
RT Arabidopsis.";
RL Plant Cell 28:892-910(2016).
CC -!- FUNCTION: Probable chloroplast-localized Mn(2+)/H(+) and/or Ca(2+)/H(+)
CC antiporter regulating Ca(2+), Mn(2+) and pH homeostasis.
CC {ECO:0000250|UniProtKB:Q94AX5}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane
CC {ECO:0000269|PubMed:18431481}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q9T0H9-1; Sequence=Displayed;
CC -!- SIMILARITY: Belongs to the GDT1 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BX827397; Type=Miscellaneous discrepancy; Note=Sequencing errors.; Evidence={ECO:0000305};
CC Sequence=CAB41117.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB78401.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL049656; CAB41117.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161536; CAB78401.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE83299.1; -; Genomic_DNA.
DR EMBL; BX827397; -; NOT_ANNOTATED_CDS; mRNA.
DR PIR; T06661; T06661.
DR RefSeq; NP_193095.2; NM_117433.5. [Q9T0H9-1]
DR AlphaFoldDB; Q9T0H9; -.
DR STRING; 3702.AT4G13590.2; -.
DR TCDB; 2.A.106.1.7; the ca(2+):h(+) antiporter-2 (caca2) family.
DR PaxDb; Q9T0H9; -.
DR PRIDE; Q9T0H9; -.
DR EnsemblPlants; AT4G13590.1; AT4G13590.1; AT4G13590. [Q9T0H9-1]
DR GeneID; 826992; -.
DR Gramene; AT4G13590.1; AT4G13590.1; AT4G13590. [Q9T0H9-1]
DR KEGG; ath:AT4G13590; -.
DR Araport; AT4G13590; -.
DR TAIR; locus:2140872; AT4G13590.
DR eggNOG; KOG2881; Eukaryota.
DR HOGENOM; CLU_040186_1_0_1; -.
DR InParanoid; Q9T0H9; -.
DR OMA; GNESHNV; -.
DR PhylomeDB; Q9T0H9; -.
DR PRO; PR:Q9T0H9; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q9T0H9; baseline and differential.
DR GO; GO:0009507; C:chloroplast; HDA:TAIR.
DR GO; GO:0009941; C:chloroplast envelope; IDA:TAIR.
DR GO; GO:0009706; C:chloroplast inner membrane; HDA:TAIR.
DR GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015085; F:calcium ion transmembrane transporter activity; IGI:TAIR.
DR GO; GO:0005384; F:manganese ion transmembrane transporter activity; IGI:TAIR.
DR GO; GO:0046873; F:metal ion transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0070588; P:calcium ion transmembrane transport; IGI:TAIR.
DR GO; GO:0019722; P:calcium-mediated signaling; IEP:TAIR.
DR GO; GO:0032468; P:Golgi calcium ion homeostasis; IBA:GO_Central.
DR GO; GO:0032472; P:Golgi calcium ion transport; IBA:GO_Central.
DR GO; GO:0071421; P:manganese ion transmembrane transport; IGI:TAIR.
DR InterPro; IPR001727; Gdt1.
DR PANTHER; PTHR12608; PTHR12608; 1.
DR Pfam; PF01169; UPF0016; 2.
DR PROSITE; PS01214; UPF0016; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Chloroplast; Membrane; Plastid; Reference proteome;
KW Transit peptide; Transmembrane; Transmembrane helix.
FT TRANSIT 1..66
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 67..359
FT /note="Protein PAM71-homolog, chloroplastic"
FT /id="PRO_0000398765"
FT TRANSMEM 110..130
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 149..169
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 177..197
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 207..227
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 269..289
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 311..331
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 339..359
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 71..102
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 79..100
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 359 AA; 37929 MW; DE8C99B30EFED4EB CRC64;
MKLTSLSKNA NSTATAVTVS SIQKLPFLSL SETLPCPKSS RKPTFLPLRC RRRPKLDLLW
GKFRVRASDA GVGSGSYSGG EEDGSQSSSL DQSPATSSES LKPRGPFPYS LSIALVLLSC
GLVFSLITFV KGGPSSVLAA VAKSGFTAAF SLIFVSEIGD KTFFIAALLA MQYEKTLVLL
GSMGALSLMT ILSVVIGKIF QSVPAQFQTT LPIGEYAAIA LLMFFGLKSI KDAWDLPPVE
AKNGEETGIE LGEYSEAEEL VKEKASKKLT NPLEILWKSF SLVFFAEWGD RSMLATVALG
AAQSPLGVAS GAIAGHLVAT VLAIMGGAFL ANYISEKLVG YVGGALFLVF AAATFFGVF
