ID   PA99_PSEAE              Reviewed;         401 AA.
AC   Q9HUG6;
DT   19-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 97.
DE   RecName: Full=Putative lipopolysaccharide biosynthesis protein PA4999;
GN   OrderedLocusNames=PA4999;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
CC   -!- FUNCTION: Potential O-antigen polymerase, which may be involved in the
CC       synthesis of LPS. {ECO:0000250}.
CC   -!- PATHWAY: Bacterial outer membrane biogenesis; LPS core biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the O-antigen polymerase family. {ECO:0000305}.
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DR   EMBL; AE004091; AAG08384.1; -; Genomic_DNA.
DR   PIR; D83022; D83022.
DR   RefSeq; NP_253686.1; NC_002516.2.
DR   RefSeq; WP_003114546.1; NZ_QZGE01000002.1.
DR   AlphaFoldDB; Q9HUG6; -.
DR   STRING; 287.DR97_2354; -.
DR   PaxDb; Q9HUG6; -.
DR   PRIDE; Q9HUG6; -.
DR   EnsemblBacteria; AAG08384; AAG08384; PA4999.
DR   GeneID; 881661; -.
DR   KEGG; pae:PA4999; -.
DR   PATRIC; fig|208964.12.peg.5239; -.
DR   PseudoCAP; PA4999; -.
DR   HOGENOM; CLU_055393_0_0_6; -.
DR   OMA; RLSIWHH; -.
DR   BioCyc; PAER208964:G1FZ6-5115-MON; -.
DR   UniPathway; UPA00958; -.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0098567; C:periplasmic side of plasma membrane; IDA:PseudoCAP.
DR   GO; GO:0005886; C:plasma membrane; IDA:PseudoCAP.
DR   GO; GO:0016757; F:glycosyltransferase activity; IDA:PseudoCAP.
DR   GO; GO:0016874; F:ligase activity; IDA:PseudoCAP.
DR   GO; GO:0008754; F:O antigen ligase activity; IMP:PseudoCAP.
DR   GO; GO:0044780; P:bacterial-type flagellum assembly; IMP:PseudoCAP.
DR   GO; GO:0071977; P:bacterial-type flagellum-dependent swimming motility; IMP:PseudoCAP.
DR   GO; GO:0009244; P:lipopolysaccharide core region biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0009243; P:O antigen biosynthetic process; IMP:PseudoCAP.
DR   GO; GO:0009297; P:pilus assembly; IMP:PseudoCAP.
DR   GO; GO:0043107; P:type IV pilus-dependent motility; IMP:PseudoCAP.
DR   InterPro; IPR007016; O-antigen_ligase-related.
DR   Pfam; PF04932; Wzy_C; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Ligase; Lipopolysaccharide biosynthesis; Membrane;
KW   Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..401
FT                   /note="Putative lipopolysaccharide biosynthesis protein
FT                   PA4999"
FT                   /id="PRO_0000208068"
FT   TRANSMEM        17..37
FT                   /note="Helical; Name=1"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        46..66
FT                   /note="Helical; Name=2"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        69..89
FT                   /note="Helical; Name=3"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        106..126
FT                   /note="Helical; Name=4"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        130..150
FT                   /note="Helical; Name=5"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        162..182
FT                   /note="Helical; Name=6"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        190..210
FT                   /note="Helical; Name=7"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        236..256
FT                   /note="Helical; Name=8"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        309..329
FT                   /note="Helical; Name=9"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        339..359
FT                   /note="Helical; Name=10"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        370..390
FT                   /note="Helical; Name=11"
FT                   /evidence="ECO:0000255"
FT   REGION          258..319
FT                   /note="WZY-C"
SQ   SEQUENCE   401 AA;  44399 MW;  1FC07DBAB8369D5B CRC64;
     MFAATRLSRL RHDTSRILSH WILPLGWLAL LTGMFWVGDR SDYHRLFYIL LAAPTLLYVI
     LQPRLLRPLT GSPLFIAFLA FSSYMMLSLS WSTPENSTGS LLKRPLYIAL LFFCAAILAL
     EAPLRLKTAT WLAALGAVIS AAATLLRYYW DANPLRLTGY GALYNPLLSA HVYGAFTALW
     LAYWMQSRPI LAPLPLISLA LLGGLLIATG SRTPLVGLTA ALMWLVLAGD RKKALIALAL
     ALAGALLGYI LYPEVITQRG ASFRPEIWAD ALRQISEHPW LGHGYDHPMR IVLSNGMLLA
     DPHNIELGVL FAGGIIGLLL WVAIYALAFG FSWKNRKSPA VLLASTWLVF GLAAGLTEGN
     AFLPRPKEHW FLIWIPMALL YALWIQQRFA ASRRGEDIAA P