PAAX_ECOLI
ID PAAX_ECOLI Reviewed; 316 AA.
AC P76086; O53019; Q2MBC7;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Transcriptional repressor PaaX;
GN Name=paaX; Synonyms=ydbY; OrderedLocusNames=b1399, JW1394;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION IN PHENYLACETATE
RP CATABOLISM.
RC STRAIN=W / ATCC 11105 / DSM 1900;
RX PubMed=9748275; DOI=10.1074/jbc.273.40.25974;
RA Ferrandez A., Minambres B., Garcia B., Olivera E.R., Luengo J.M.,
RA Garcia J.L., Diaz E.;
RT "Catabolism of phenylacetic acid in Escherichia coli. Characterization of a
RT new aerobic hybrid pathway.";
RL J. Biol. Chem. 273:25974-25986(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP FUNCTION AS A TRANSCRIPTIONAL REPRESSOR, REGULATION BY PA-COA, AND
RP CONSENSUS MOTIF.
RX PubMed=10766858; DOI=10.1074/jbc.275.16.12214;
RA Ferrandez A., Garcia J.L., Diaz E.;
RT "Transcriptional regulation of the divergent paa catabolic operons for
RT phenylacetic acid degradation in Escherichia coli.";
RL J. Biol. Chem. 275:12214-12222(2000).
CC -!- FUNCTION: Negative regulator of the paaZ and paaABCDEFGHIJK catabolic
CC operons. Binds the consensus sequence 5'-WWTRTGATTCGYGWT-3'. Binding of
CC PaaX is specifically inhibited by phenylacetyl-coenzyme A (PA-CoA).
CC {ECO:0000269|PubMed:10766858, ECO:0000269|PubMed:9748275}.
CC -!- PATHWAY: Aromatic compound metabolism; phenylacetate degradation.
CC -!- INTERACTION:
CC P76086; P0AE82: cpxA; NbExp=3; IntAct=EBI-544692, EBI-9141330;
CC P76086; P41443: gspH; NbExp=2; IntAct=EBI-544692, EBI-1129978;
CC -!- SIMILARITY: Belongs to the PaaX family. {ECO:0000305}.
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DR EMBL; X97452; CAA66101.1; -; Genomic_DNA.
DR EMBL; U00096; AAC74481.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE76429.1; -; Genomic_DNA.
DR PIR; B64891; B64891.
DR RefSeq; NP_415917.1; NC_000913.3.
DR RefSeq; WP_000039887.1; NZ_SSZK01000012.1.
DR AlphaFoldDB; P76086; -.
DR SMR; P76086; -.
DR BioGRID; 4263014; 16.
DR BioGRID; 850329; 6.
DR DIP; DIP-10431N; -.
DR IntAct; P76086; 21.
DR STRING; 511145.b1399; -.
DR jPOST; P76086; -.
DR PaxDb; P76086; -.
DR PRIDE; P76086; -.
DR EnsemblBacteria; AAC74481; AAC74481; b1399.
DR EnsemblBacteria; BAE76429; BAE76429; BAE76429.
DR GeneID; 945966; -.
DR KEGG; ecj:JW1394; -.
DR KEGG; eco:b1399; -.
DR PATRIC; fig|1411691.4.peg.872; -.
DR EchoBASE; EB3509; -.
DR eggNOG; COG3327; Bacteria.
DR HOGENOM; CLU_067515_0_0_6; -.
DR InParanoid; P76086; -.
DR OMA; LLIHEYR; -.
DR PhylomeDB; P76086; -.
DR BioCyc; EcoCyc:G6720-MON; -.
DR UniPathway; UPA00930; -.
DR PRO; PR:P76086; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IDA:EcoCyc.
DR GO; GO:2000143; P:negative regulation of DNA-templated transcription, initiation; IMP:EcoCyc.
DR GO; GO:0010124; P:phenylacetate catabolic process; IMP:UniProtKB.
DR GO; GO:0006351; P:transcription, DNA-templated; IDA:EcoCyc.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR012906; PaaX-like_N.
DR InterPro; IPR013225; PaaX_C.
DR InterPro; IPR011965; PaaX_trns_reg.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR Pfam; PF07848; PaaX; 1.
DR Pfam; PF08223; PaaX_C; 1.
DR PIRSF; PIRSF020623; PaaX; 1.
DR TIGRFAMs; TIGR02277; PaaX_trns_reg; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Reference proteome; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..316
FT /note="Transcriptional repressor PaaX"
FT /id="PRO_0000058165"
FT VARIANT 5
FT /note="D -> V (in strain: W)"
FT VARIANT 311
FT /note="L -> I (in strain: W)"
FT VARIANT 315
FT /note="I -> T (in strain: W)"
SQ SEQUENCE 316 AA; 35295 MW; 286C97DBC2564030 CRC64;
MSKLDTFIQH AVNAVPVSGT SLISSLYGDS LSHRGGEIWL GSLAALLEGL GFGERFVRTA
LFRLNKEGWL DVSRIGRRSF YSLSDKGLRL TRRAESKIYR AEQPAWDGKW LLLLSEGLDK
STLADVKKQL IWQGFGALAP SLMASPSQKL ADVQTLLHEA GVADNVICFE AQIPLALSRA
ALRARVEECW HLTEQNAMYE TFIQSFRPLV PLLKEAADEL TPERAFHIQL LLIHFYRRVV
LKDPLLPEEL LPAHWAGHTA RQLCINIYQR VAPAALAFVS EKGETSVGEL PAPGSLYFQR
FGGLNIEQEA LCQFIR
